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A pachyrhizus erosus fermentation product and application thereof to preparation of a composition for improving gene expression quantity and reducing skin melanin content

A fermented product and a technology of jicama can be applied to a composition for reducing skin melanin content to prepare and improve gene expression. , to achieve the effect of increasing skin anti-aging activity, increasing skin antioxidant activity, and increasing skin firmness

Active Publication Date: 2020-04-17
百岳特生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, most of the skin anti-aging products on the market can only increase the antioxidant activity, and cannot directly and effectively improve or delay the occurrence of skin aging
[0004] In addition, in recent years, in order to meet people's pursuit of fair skin and fewer spots, various whitening products that inhibit the formation of melanin or lighten spots have been continuously developed on the market. However, in order to achieve special effects, these products add too many carcinogenic chemicals or environmental hormones, so that consumers use ingredients that will damage the skin and affect health without knowing it

Method used

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  • A pachyrhizus erosus fermentation product and application thereof to preparation of a composition for improving gene expression quantity and reducing skin melanin content
  • A pachyrhizus erosus fermentation product and application thereof to preparation of a composition for improving gene expression quantity and reducing skin melanin content
  • A pachyrhizus erosus fermentation product and application thereof to preparation of a composition for improving gene expression quantity and reducing skin melanin content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation method of cold potato fermented product of the present invention

[0035] Pachyrhizus erosus is a leguminous (Legumen) legume genus (Pachyrhizus) perennial vine-like herb, also known as yam bean, pueraria lobata, and pueraria lobata. Potato tubers can be eaten raw or cooked, with a diameter of about 20-30 cm. They are rich in sugar and protein. They can be processed into sago powder, which has the effect of cooling and removing heat.

[0036] In an embodiment of the present invention, the cold potato is thoroughly washed, and the washed cold potato is mixed with water at a weight ratio of 1:5-10, and sterilized and extracted at 50-100°C for 0.5-1.5 hours to obtain Cooled potato water extract. The cold potato water extract is cooled to room temperature for subsequent three-stage fermentation. It is first colonized with 0.01-0.5% yeast (Saccharomyces cerevisiae, purchased from the Biological Resources Conservation and Research Center, Taiwan, China, numbe...

Embodiment 2

[0037] Example 2 The total polyphenol content of the fermented cold potato of the present invention is increased

[0038] This example is to compare whether the total polyphenol content in the cold potato fermentation of the present invention is higher than that in the cold potato water extract. The Folin-Ciocalteu colorimetric method is used to determine the total polyphenol content. The determination method uses the tungsten in the reagent. Molybdic acid quantifies the total polyphenol content, which itself is reduced (by Mo 6+ Becomes Mo 5+ ) Will produce a blue compound, and the depth of the blue compound is positively correlated with the total polyphenol content. First, the cold potato fermented product and cold potato water extract of the present invention were diluted 1 times with water, and 100 mL was taken into a centrifuge tube. Then, 500 μL of Folin-Ciocalteu phenol reagent was added to mix and let stand for 3 minutes. Then, 400 μL of After mixing with 7.5% sodium carb...

Embodiment 3

[0040] Example 3 The ability of the cold potato fermented product of the present invention to reduce the melanin content of human skin fibroblasts

[0041] This example is to compare whether the cold potato fermented product of the present invention has a higher ability to reduce the melanin content than the cold potato water extract. Therefore, melanoma cells (B16F10, No. CRL-6475 TM ) Test, using 10% Fetal Bovine Serum, 1% Penicillin-Streptomycin (purchased from Gibco, USA), and 90% DMEM (Dulbecco's Modified Eagle Medium, purchased from Gibco, U.S., 12100-046) culture medium for cell culture. First, add 3 mL of culture medium to each well of the 6-well culture plate and implant 1.5×10 5 After culturing the cells at 37°C for 24 hours, remove the culture medium without disturbing the attached cells, and then divide the cells into the following four groups (n=4 for each group): (1) blank control group, (2 ) 0.25% kojic acid is the positive control group, (3) 0.25% cold potato wa...

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Abstract

The invention relates to a biological fermentation product, in particular to a pachyrhizus erosus fermentation product and application thereof to preparation of a composition for improving gene expression quantity and reducing skin melanin content. The pachyrhizus erosus fermentation product provided by the invention is prepared by carrying out three-stage fermentation on a pachyrhizus erosus water extract by using saccharomycetes, lactobacillus and acetobacter aceti. The pachyrhizus erosus fermentation product is used for preparing a composition for improving COL gene, TIMP gene, LOX gene, ELN gene, HAS gene, SOD gene, TCP1 gene and UNG gene, and reducing skin melanin content. The composition can effectively increase the anti-aging activity of the skin, increase the firmness of the skin and improve the whitening ability of the skin, and achieves the comprehensive skin health-care effect.

Description

Technical field [0001] The present invention relates to biological fermented products, especially cold potato fermented products and their use for preparing compositions that enhance gene expression and reduce skin melanin content. Background technique [0002] The epidermal layer is the outermost layer of the skin. It is the stratum corneum, the granular layer, the spinous layer and the basal layer from the outside to the inside. The epidermal layer is mainly formed by the continuous upward differentiation of undifferentiated cylindrical keratinocytes in the basal layer. The process is called keratinization. Keratinocytes have a high water content. As the cells are metabolized and differentiated, the shape of the keratinocytes will gradually become flat, and the nucleus and organelles will begin to degenerate and shrink, and dead cells without nuclei and organelles will be formed in the stratum corneum. The main function is to make the skin retain water and form a skin barrier ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/00A61Q19/08A61Q19/02
CPCA61K8/9789A61Q19/00A61Q19/08A61Q19/02A61K2800/85
Inventor 林咏翔
Owner 百岳特生物技术(上海)有限公司
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