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Application of bacterial strain

A technology of reaction and Shewanella, applied in the field of environmental engineering, can solve problems such as environmental pollution

Active Publication Date: 2018-05-08
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Animals that eat organic arsenic additives for a long time will produce a certain amount of inorganic arsenic in their bodies. Although it has not been confirmed whether this level of inorganic arsenic is harmful to humans, the harm of inorganic arsenic entering the human body through meat cannot be ignored; the most noteworthy is Most of the phenylarsine compounds enter the soil and surface water with excrement without any change in the animal body. The originally low-toxic phenylarsine compounds undergo long-term environmental transformation to produce highly toxic inorganic arsenic, which will directly pollute the environment
[0007] Among the four common phenylarsine compounds, p-nitrophenylarsine acid was banned late, and the microbial treatment method for this organic arsine compound has not been reported, so finding an effective treatment for aquaculture wastewater and other The method of p-nitrophenylarsinic acid in the environment has important practical significance

Method used

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  • Application of bacterial strain
  • Application of bacterial strain
  • Application of bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1, Shewanella oneidensis MR-1 degrades p-nitrophenylarsine under anaerobic conditions

[0048] The formulation of the LB medium used in this example is as follows: 10g / L peptone, 10g / L NaCl, 5 g / L yeast extract, autoclaved at 121°C for 20min and then used for the expanded culture of strains.

[0049] The main components of the medium used for anaerobic culture are as follows: 11.91g / L HEPES buffer salt, NaCl 0.46g, K 2 HPO 4 0.05g / L, KH 2 PO 4 0.035g / L, (NH 4 ) 2 SO 4 0.225g / L, MgSO 4 ·H 2 O 0.117g / L and trace element mixture (pH=7.2). Add sodium lactate as the only electron donor in the anaerobic medium to maintain bacterial growth (final concentration 5mM / L) in this example.

[0050] The anaerobic reaction system used in this example is a serum bottle containing 30 mL of mineral salt medium. After 15 minutes of nitrogen exposure, the serum bottle is sealed with a butyl rubber stopper and an aluminum cap to ensure a stable anaerobic reaction system....

Embodiment 2

[0056] Example 2, Degradation products of Shewanella oneidensis MR-1 p-nitrophenylarsine and degradation mechanism analysis under anaerobic conditions

[0057] In this example, the qualitative analysis of the degradation products is mainly carried out by various analytical means.

[0058] 1. High performance liquid chromatography detection of the product

[0059] The sample described in the previous example is used for the detection of high performance liquid chromatography and hydride generation-atomic fluorescence detector after being filtered through a 0.22 μm filter membrane. High-performance liquid chromatography (HPLC, Essentia LC-16, SHIMADZA, Japan) analysis conditions are: chromatographic column (ZORBAX Eclipse Plus C18: 4.6 × 250 nm, 5 μm, Agilent, USA), mobile phase is 10mM / L Potassium Dihydrogen Phosphate Solution (pH7.2), the measurement flow rate is 1.0mL / min, the test injection volume is 20μL, the test wavelength of p-nitrophenylarsonic acid and arsanic acid a...

Embodiment 3

[0075] Example 3, Toxicity detection of Shewanella oneidensis MR-1 p-nitrophenylarsine acid degradation products

[0076] In this example, an autoluminescent bacterium, Salmonella typhimurium Sal94, was used to detect the toxicity of the degradation product of p-nitrophenylarsine from Shewanellaoneidensis MR-1.

[0077] Salmonella typhimurium Sal94 is a model strain widely used in the detection of genotoxicity in water samples. The more toxic the substance contained in the water body, the lower the intensity of its autoluminescence, which is a sensitive method for evaluating product toxicity.

[0078] The activation of the Salmonella typhimurium Sal94 bacterial strain in this example is as follows: when the bacterial strain is activated in LB medium, it is necessary to add 30 μg / ml of kanamycin to ensure the stability of the plasmid, overnight in a constant temperature shaker (30 ° C, 200 rpm) After culturing, inoculate into fresh LB medium with an inoculum size of 1:100 and c...

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Abstract

The invention relates to the field of environmental engineering, in particular to application of an anaerobic microorganism for treating organic arsine pollutants in a water environment. The inventiondiscloses a method for removing p-nitrophenylarsonic acid in the water environment by using shewanella bacteria, complete degradation of the pollutant such as p-nitrophenylarsonic acid inside water is completed under anaerobic conditions through an Mtr electron transfer way by using the dissimilatory metal reduction capacity of Shewanella oneidensis MR-1, and arsanilic acid with relatively low toxicity is generated, so that the target of reducing the potential environmental threat of p-nitrophenylarsonic acid is realized. The Shewanella oneidensis MR-1 is the first strain of bacteria capableof being applied to degrading of p-nitrophenylarsonic acid, and has the advantage that the bacterial strain has the effect of efficiently degrading p-nitrophenylarsonic acid in water; and moreover, main function proteins proposed in the invention can provide an important reference for the improvement of engine engineering strains.

Description

technical field [0001] The invention relates to the field of environmental engineering, in particular to utilizing an anaerobic microorganism to treat organic arsine pollutants in a water environment. Background technique [0002] As a metalloid element, arsenic is widely distributed in nature and is usually combined with various metal minerals, such as copper and iron. Arsenic and its compounds are highly toxic and are widely used in pesticides, herbicides, and insecticides. In addition, the mining and smelting of arsenic and arsenic-containing metals, the production of glass, pigments, technical materials, and paper using arsenic or arsenic compounds as raw materials, and the burning of coal produce arsenic-containing wastewater, waste gas and waste residue, which pollute the environment . Wastewater containing arsenic, pesticides and soot can also pollute the soil, causing arsenic to accumulate in the soil and thus enter crop tissues and eventually enrich in the food ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/28C02F101/30
CPCC02F3/2806C02F3/34C02F2101/30
Inventor 俞汉青黄雪娜刘东风
Owner UNIV OF SCI & TECH OF CHINA
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