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Preparation method of polyaniline-loaded gamma-PGA (Polyglutamic Acid) nano-hydrogel

A technology of nano-hydrogel and polyaniline is applied in the field of preparation of γ-PGA nano-hydrogel to achieve the effects of uniform particle size distribution, good water solubility and simple operation.

Active Publication Date: 2017-06-16
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] A search of domestic and foreign literature found that there is no relevant report on the preparation of γ-PGA hydrogel using cystamine dihydrochloride as a crosslinking agent for loading polyaniline as a photothermal reagent.

Method used

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  • Preparation method of polyaniline-loaded gamma-PGA (Polyglutamic Acid) nano-hydrogel
  • Preparation method of polyaniline-loaded gamma-PGA (Polyglutamic Acid) nano-hydrogel
  • Preparation method of polyaniline-loaded gamma-PGA (Polyglutamic Acid) nano-hydrogel

Examples

Experimental program
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Effect test

Embodiment 1

[0044] (1) Dissolve 20mg of γ-PGA in 1mL of ultrapure water, add 30mg of EDC to activate for 1-2h, then add 26mg of NaHCO 3 The reaction was continued for 0.5h; then the above solution was added dropwise to the DCM solution (4mL) in which 134mg of AOT was dissolved, and stirred for 10min to form a W / O emulsion; then the W / O emulsion was added dropwise to 30mL containing 612mg of PVA ( Alcoholysis degree 87-89%, Mw=85-124kDa) in ultrapure aqueous solution, stir 15min, form the polymer emulsion of W / O / W; (2) 60mg cystamine dihydrochloride is dissolved in 1.2mL ultrapure Pure water, and added dropwise to the W / O / W polymer emulsion prepared in the above steps, stirred for 12 hours, evaporated overnight to remove the organic solvent, and then purified by dialysis for 3 days with a dialysis bag with a molecular weight cut-off of 100,000, and frozen dry to obtain cystamine dihydrochloride cross-linked γ-PGA / Cys nanometer hydrogel. (3) Dissolve 48 mg of γ-PGA / Cys nano hydrogel in 60 ...

Embodiment 2

[0046] Take the γ-PGA / Cys@PANI nanohydrogel prepared in Example 1, dilute it to an appropriate concentration with ultrapure water, and use it to measure the surface potential and hydrodynamic diameter. Zeta potential measurement results showed that the surface potential of γ-PGA / Cys@PANI nanohydrogel was -4.32±0.16mV. The hydrodynamic diameter of γ-PGA / Cys@PANI nano hydrogel is 669.03±14.99nm, the particle size distribution is uniform, and the hydrodynamic diameter can remain almost unchanged for a long time, and it can be stored stably in different solvents for a certain period of time ( see figure 1 ), thus illustrating that the γ-PGA / Cys@PANI nanohydrogel prepared in Example 1 has good colloidal stability.

[0047] figure 1 In order to disperse the γ-PGA / Cys@PANI nano hydrogel in water, 0.9% NaCl, PBS and cell culture medium, measure the hydrodynamic particle size after storing for different time, it was found that the particle size in water remained at about 660nm, Th...

Embodiment 3

[0049] The γ-PGA / Cys@PANI nano hydrogel obtained in Example 1 was taken to measure the ultraviolet absorption (see figure 2 : UV absorption spectrum of γ-PGA / Cys@PANI nanohydrogel at 400 to 1100 nm). From figure 2 It can be seen that the prepared hydrogel has an obvious absorption peak at 700nm and a strong ultraviolet absorption value at 785nm, which provides feasibility for near-infrared imaging and photothermal therapy. In addition, at 785nm laser (1.5W / cm 2 ) were irradiated for different times, the absorption value of the nanogel at 785nm did not change significantly, indicating that the prepared nanoparticles have good photothermal stability and can be used for long-term photothermal treatment of diseases under laser exposure ( image 3 ). Observe the morphology of the γ-PGA / Cys@PANI nanohydrogel prepared in Example 1 by TEM (see Figure 4 ), the results show that the formed γ-PGA / Cys@PANI nano-hydrogel has a spherical shape and a relatively uniform size. Obvious ...

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Abstract

The invention relates to a preparation method of polyaniline-loaded gamma-PGA (Propylene Glycol Alginate) nano-hydrogel. The preparation method comprises the following steps: carrying out double emulsification reaction on EDC (Dichloroethane)-activated gamma-PGA to obtain a W / O / W polymer emulsion; dissolving cystamine dihydrochloride into ultrapure water, then dropwise adding a mixed solution into the W / O / W polymer emulsion, and reacting to obtain gamma-PGA / Cys nano-hydrogel; dissolving the gamma-PGA / Cys nano-hydrogel into ultrapure water with a pH value of 3.00, then adding the cystamine dihydrochloride, standing in an ice bath for 44 minutes to 1 hour, then adding ammonium persulfate for reacting, dialyzing and purifying to obtain a finished product. The preparation method disclosed by the invention has the advantages of simple process, easiness in operation and separation, wide source of raw materials and low cost; the prepared gamma-PGA hydrogel is uniform in particle size distribution and has the advantages of good water solubility, colloidal stability, biocompatibility, no adverse influence on a living body and potential application value in the field of photothermal therapy.

Description

technical field [0001] The invention belongs to the field of preparation of nano hydrogels, in particular to a preparation method of gamma-PGA nano hydrogels loaded with polyaniline. Background technique [0002] For cancer, the current treatment methods include surgical treatment, radiotherapy and chemical drug treatment, but these traditional treatment methods still have defects. Photothermal therapy is a new technology for treating tumors. It is precise, controllable and efficient. The tumor tissue is selectively irradiated with near-infrared light, and heat is generated in its area, and the temperature rises, leading to the death of cancer cells. Therefore, novel biomedical materials for high-efficiency photothermal therapy are of great significance in tumor therapy. Photothermal materials are divided into organic and inorganic materials. Inorganic materials such as metal nanoparticles have poor biological metabolism, long-term toxicity, and toxic reactions induced by c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K41/00A61K47/69A61P35/00B82Y5/00B82Y20/00C08G73/02
CPCA61K41/0052A61K49/226B82Y5/00B82Y20/00C08G73/0266
Inventor 史向阳周一伟周本青孙文杰胡勇
Owner DONGHUA UNIV
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