Novel double-layered composite transmitting tissue regeneration membrane and preparation method thereof

Abstract

The invention relates to a novel double-layered composite transmitting tissue regeneration membrane and a preparation method thereof. The double-layered composite transmitting tissue regeneration membrane consists of a PLGA / hydroxyapatite nonporous dense membrane on the outer layer and a PLGA / woolen keratin electrostatic spinning porous membrane on the inner layer. By means of a solvent casting method and a high-voltage electrostatic spinning method, the synthetic material PLGA is combined with the natural component woolen keratin and the inorganic component hydroxyapatite to form the composite material which supplement each other with respective advantages, thereby forming the PLGA / hydroxyapatite / woolen keratin double-layered composite transmitting tissue regeneration membrane. The double-layered composite transmitting tissue regeneration membrane disclosed by the invention is simple in preparation method and moderate in membrane forming conditions, has a good cell biocompatibility, conforms to the requirement of application in the living organism, and has a good application prospect as a novel transmitting tissue regeneration membrane.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a novel double-layer composite guiding tissue regeneration membrane and a preparation method thereof. Background technique [0002] Guided Tissue Regeneration (GTR) is a biological concept proposed by Nyman et al. in the early 1980s, which was initially applied to the treatment of periodontal disease. It refers to placing a barrier film between the gingival flap and the root bone surface to block the contact between the gingival epithelium and connective tissue and the root surface, so that the periodontal ligament precursor cells that have the ability to form new attachments can move along the root surface during the healing process. Surface growth, re-form cementum, alveolar bone and periodontal fibers, establish new attachments, and achieve periodontal tissue regeneration. Urist and Mclean found that after bone damage, different tissue cells migrated to the defect at diff...

AI Technical Summary

Problems solved by technology

[0007] Degradable materials mainly include natural collagen and artificial polymers (polylactic acid (PLA), polyglycolic acid (PGA), polyglycolide-lactide copolymer (PLGA), polyhydroxybutyrate, etc.) etc., their biological performance and degradation performance can meet the needs of GTR, without the pain and cost of secondary surgery, ensure that the tissue regeneration process is not disturbed, and the clinical effect is equivalent to or better than non-degradable materials, so the application can be Degradable membranes have become a development trend of GTR, but degradable membranes generally have disadvantages such as insufficient hydrophilicity of materials, weak adhesion to cells, and poor tissue engagement; degradation products are acidic, which is not conducive to cell growth and tissue repair.

For example, collagen film has good cell affinity and biocompatibility, and has been used in medicine to repair the defects of gums, bones and other tissues. However, the defects of collagen film are: poor mechanical properties, low mechanical strength, and low price. Expensive, and the degradation rate is not easy to adjust, it cannot match the repair rate of different tissues, and it will rupture before new bone formation, causing early epithelial cells to dive and lead to GTR treatment failure

Currently, the most studied absorbable polymer film is PLA or PLGA. This kind of polymer can adjust the degradation rate by changing its molecular weight and composition and has good tissue compatibility. However, PLA and PLGA are hydrophobic materials, and the degradation rate is too fast. slow, may affect the proliferation and migration of bone cells, and the lactic acid produced by its degradation products is likely to cause inflammation in the surrounding tissues, which has an adverse effect on the formation of new bone

Poly-β-hydroxybutyric acid has good mechanical properties and biocompatibility, and the degradation time is 3 months. The application of poly-β-hydroxybutyric acid film to dog implant surgery and lower collarbone expansion experiments performed well The ability to guide bone tissue regeneration, but the disadvantage is that it can only play the role of mechanical barrier and isolation but does not have the role of actively inducing differentiation and promoting growth, which affects and limits its application.

[0010] At present, collagen membranes mainly represented by Bio-Gide are widely used in foreign countries. Due to their high price, they are difficult to use in large quantities in China. However, domestically produced membranes for guiding tissue regeneration have complex processes, high prices, no osteoinductive effect, Insufficient hydrophilicity of the material, weak adhesion to cells, poor tissue cohesion, and acidic degradation products, which are not conducive to cell growth and proliferation, tissue repair, and unstable osteogenesis, etc.

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