Chitosan modified alginate hydrogel three-dimensional porous bracket and preparation method thereof
A three-dimensional porous, porous scaffold technology, applied in medical science, prosthesis and other directions, can solve the problems that need to be further improved, and achieve the effects of improving cell affinity, rich source of raw materials, cheap and easy to obtain
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Embodiment 1
[0023] (1) Weigh 0.40 g of sodium alginate and dissolve it in 10 mL of phosphate buffer solution (pH=5), and stir at room temperature until the sodium alginate is completely dissolved.
[0024] (2) Add 0.87 g of EDC to the solution (1), and stir at 4° C. for 30 min.
[0025] (3) Add 0.28 g of cystamine to the solution (2) as a cross-linking agent, stir and fully dissolve, pour the reaction solution into a plastic culture plate, and place it at room temperature for 3 hours to form a hydrogel.
[0026] (4) The hydrogel obtained in step (3) was pre-frozen in a refrigerator at -50°C for 24 hours, and then freeze-dried for 2 days to obtain a porous scaffold material. The swelling rate of this unmodified three-dimensional porous scaffold in deionized water is about 35g / g, and the degradation time in 50mM cysteine solution is about 15h.
[0027] (5) Soak the porous scaffold obtained in step (4) in a 3 mg / mL chitosan (molecular weight 5k) solution for 4 hours, so that the chitosan ...
Embodiment 2
[0030] Raw material, preparation method are substantially identical with embodiment 1. It is 0.56g that the step (3) among the embodiment 1 is adjusted to cystamine addition, and the chitosan molecular weight in the step (5) is adjusted to 10k, and other steps are identical. The swelling ratio of the unmodified three-dimensional porous scaffold is about 24g / g. The swelling rate of the three-dimensional porous scaffold modified by chitosan solution was 6g / g.
Embodiment 3
[0032] Raw material, preparation method are substantially identical with embodiment 1. Step (1) in Example 1 was adjusted to an addition of sodium alginate of 0.45 g, and step (2) was adjusted to an addition of EDC of 0.96 g. The unmodified 3D porous scaffold has low strength and can barely maintain its shape with a swelling rate of about 29 g / g. However, the compressive strength of the modified three-dimensional porous scaffold can reach 4.5±1.3kPa, the compressive modulus is about 27.4±6.1kPa, and the swelling rate is 8g / g.
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