Composite continuous bed cryogel and preparation thereof, and application in separating IgG and albumin
A composite crystal and bed medium technology, applied in the direction of albumin peptide, serum albumin, separation methods, etc., can solve the problems of expensive Protein A, difficult to achieve large-scale separation, low adsorption capacity of bed column, etc., and achieve rapid separation process and excellent separation performance Excellent, excellent biosafety effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027]Cellulose powder (commercially available, particle size 300-1500 μm, average particle size about 800 μm) in a mixed aqueous solution of hydroxyethyl methacrylate and polyethylene glycol diacrylate (the mass of hydroxyethyl methacrylate in the mixed aqueous solution is equal to The total mass concentration of polyethylene glycol diacrylate is 15%, and the mass ratio of hydroxyethyl methacrylate to polyethylene glycol diacrylate is 3:1). Add 4.25g of saturated cellulose particles to 1.18mL Mixed aqueous solution of hydroxyethyl methacrylate and polyethylene glycol diacrylate (the total mass concentration of hydroxyethyl methacrylate and polyethylene glycol diacrylate in the mixed aqueous solution is 15%, and the mass ratio is 3:1) , then add 0.089g of catalyst made by mixing ammonium persulfate and tetramethyldiethylamine at a mass ratio of 1:1, and conduct crystallization and polymerization at -15°C for 20 hours to prepare polyhydroxyethyl methacrylate The matrix and the ...
Embodiment 2
[0030] Cellulose powder (commercially available, particle size 300-1900 μm, average particle size about 900 μm) in a mixed aqueous solution of hydroxyethyl methacrylate and polyethylene glycol diacrylate (the mass of hydroxyethyl methacrylate in the mixed aqueous solution is equal to The total mass concentration of polyethylene glycol diacrylate is 15%, and the mass ratio is 3:1), and 6.5g of saturated cellulose particles are added to 1.1mL of hydroxyethyl methacrylate and polyethylene glycol diacrylate. Mixed aqueous solution (the total mass concentration of hydroxyethyl methacrylate and polyethylene glycol diacrylate in the mixed aqueous solution is 15%, and the mass ratio is 3:1), and then ammonium persulfate and tetramethyldiethylamine are added to 0.083g of the catalyst prepared by mixing at a ratio of 1:1 was prepared by crystallization and pore formation and polymerization for 20 hours at -15°C to obtain a continuous bed matrix of polyhydroxyethyl methacrylate matrix and...
Embodiment 3
[0034] Get the composite crystal gel continuous bed medium in embodiment 2, other operations are the same as embodiment 2, 0.2mL healthy human serum is mixed with 20mM phosphate (Na 2 HPO 4 / NaH 2 PO 4 ) buffer solution (pH = 6.5) was diluted 15 times, and the compound crystal gel continuous bed chromatography was performed at a flow rate of 1 cm / min, and then washed with PBS buffer solution (pH = 6.5, 20 mM) and washed with 50 mM, 150 mM and 1 M NaCl respectively Eluent (pH=6.5, 20mM Na 2 HPO 4 / NaH 2 PO 4 Buffer configuration) for step-by-step elution, collect the effluent with the elution peak when eluted with the eluent with a final concentration of 50mM NaCl, dialyze with deionized water for 24 hours through a MWCO50000 dialysis membrane, and then freeze-dry at -60°C for 24 hours , to obtain human serum albumin, SDS-PAGE electrophoresis detection purity of about 94%, Coomassie Brilliant Blue method measured adsorption capacity of 0.21mg / mL bed layer; collect the elu...
PUM
Property | Measurement | Unit |
---|---|---|
pore size | aaaaa | aaaaa |
adsorption capacity | aaaaa | aaaaa |
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information

- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com