According to the invention, early-flowering oil-used type variety 'YS-143' and late-flowering turnip variety 'VT-044' are taken as test materials, and the
flowering time of the two in two conditions,namely long
sunlight and short
sunlight, are respectively observed. The cDNA sequence of the
gene is cloned by taking two materials as a template simultaneously, and the
amino acid sequence of the
gene is analyzed by using a
bioinformatics technology, so as to find the difference loci of the CCA1 genes of the early-flowering and late-flowering Chinese cabbage crops, and the difference loci is primarily confirmed by analysis such as
bioinformatics and sequencing comparison, and then an
InDel maker is developed. The
nucleotide sequence of the
InDel maker is ACCCTT, which is located on the sixthexon of the CCA1
gene of the early-flowering Chinese cabbage
crop, but does not exist on the CCA1 gene
coding region of the late-flowering Chinese cabbage
crop. Finally, by
verification, the
InDel maker is taken as a functional marker for molecule assistant breeding operation. The
molecular marker disclosed by the invention can be simply, conveniently and rapidly applied to breeding practice withhigh
throughput.