32results about How to "Improve insecticidal virulence" patented technology

The invention discloses a novel multifunctional compound microbial fertilizer and belongs to the technical field of agricultural compound fertilizer. The novel multifunctional compound microbial fertilizer is prepared from the following raw materials: a microbial agent, biological carbon, pyrethrum powder, carbamide, monoammonium phosphate, potassium chloride, biochemical fulvic acid and EDTA chelated microelements. The novel multifunctional compound microbial fertilizer is capable of providing nutrients required for crops and is capable of excellently decreasing incidences of plant diseases and insect pests, so that the use of pesticide is reduced and the yield and the quality of the crops are improved.

The invention discloses a leaf vegetable type vegetable organic fertilizer and a preparation method thereof, and relates to the technical field of organic fertilizer. The leaf vegetable type vegetable organic fertilizer is prepared from 60-70 parts of humic acid fertilizer, 55-65 parts of plant ash, 45-55 parts of peanut meal, 45-50 parts of carrier components, 35-45 parts of cornstalk, 35-40 parts of pond sludge, 20-30 parts of urea, 20-30 parts of disinsectization components, 15-25 parts of beet, 15-20 parts of saccharose, 15-18 parts of edible mushroom dregs, 10-15 parts of organic material leavening agent, 10-12 parts of xylitol residues, 5-10 parts of pig manure, 2-8 parts of ammonium sulfate, 2-6 parts of magnesium sulfate and 90-100 parts of water. The leaf vegetable type vegetable organic fertilizer can not only promote crop growth of leaf vegetables, improve the crop quality and but also effectively prevent plant diseases and insect pests.

The invention discloses a compound pesticide, which is mainly prepared from the following raw materials in percentage by weight: 5-20% of azadirachtin and metarrhizium, 8-12% of emulsifier, 3-7% of stabilizer, 2-8% of synergist, 0.1-1.0% of antioxidant, 10-18% of cosolvent and 50-60% of organic solvent. The compound pesticide is environment-friendly, has high compatibility and obvious synergisticeffect, and can obviously improve the insecticidal toxicity of a single dosage and reduce the use of high-persistent chemical pesticide.

The invention discloses a metarhizium anisopliae transgenic strain as well as a preparation method and an application thereof. The strain is a recombinant engineering bacterium constructed by fusion of metarhizium anisopliae genes and exogenous Prla (subtilisin-like protease A) genes. The metarhizium anisopliae transgenic strain takes metarhizium anisopliae as the basis, takes Pgpd as a strong promoter, takes TTrpC as a terminator and takes Basta as a selection marker, a Prla overexpression box is constructed with a PCR fusion method, then a fungal protoplast is prepared, genetic engineering transformation is conducted with a CaCl2-PEG method, and the overexpression box is inserted in a metarhizium anisopliae gene group randomly for overexpression. The metarhizium anisopliae transgenic strain is good in German cockroach killing effect, higher in toxicity and capable of effectively controlling German cockroach populations in a short time.

The invention discloses a biogenic insecticide mixture controlling pests. The biogenic insecticide mixture is prepared from, by weight, 40-60 parts of a bacillus thuringiensis fermentation solution, 10-20 parts of a lactic acid bacteria fermented solution, 10-20 parts of fresh anisodus acutangulus leaves, 10-20 parts of fresh leaves of camphor trees and 0.05-0.1 part of Tween 20. According to the biogenic insecticide mixture, the selectivity of selected bacillus thuringiensis is high, damage to people and livestock does not exist, the biogenic insecticide mixture belongs to biological insecticide sources very high in safety, lactic acid bacteria belong to probiotics, the lactic acid bacteria are used for preparing an insecticide for the first time, the toxicity titer is high, time needed for disinsection is short, a preservative does not need to be additionally added, chemical substances are reduced, and the problem that residual time of chemical pesticide is long is solved. In addition, the invention discloses a preparation method and application of the biogenic insecticide mixture.

The invention relates to a method for acquiring high-activity Cry1Ai protein mutants and the mutants, and belongs to the biotechnology field. The method for acquiring the high-activity Cry1Ai protein mutants comprises the steps that continuous multi-point mutation is performed on an amino acid sequence in a loop 2 or / and a loop 3 of a Cry protein Domain II and comprises more than 10 continuous amino acid sequence changing. The insecticidal activity of the continuous multi-point mutant ModCry1Aid-loop 2 on cotton bollworm is improved by about 13 times, the activity of the continuous multi-point mutant ModCry1Aid-loop 2 on ostrinia nubilalis is improved by near 9 times compared with a Cry1Ai protein before improvement, and the activity of the continuous multi-point mutant ModCry1Aid-loop 2 and the continuous multi-point mutant ModCry1Aid-loop 3 on rice stem borer is improved by about 5-6 times compared with the Cry1Ai protein before improvement.

A recombinant autographa california multiple nuclear polybedrosis virus, its preparing process, and its application as insecticide with broad spectrum and high speed are disclosed.

The invention belongs to the field of biotic insecticides and relates to a microbial pesticide synergist. The main ingredient of the synergist is camptothecin or a derivative thereof. The invention relates to two microbial pesticides containing the synergist at the same time, namely a microbial pesticide containing bacillus thuringiensis and the camptothecin / camptothecin derivative, and a microbial pesticide containing baculovirus and the camptothecin / camptothecin derivative. The synergist can effectively improve the pest killing effect of the bacillus thuringiensis or baculovirus pesticide, enhance the pesticide effect and reduce the dosage of the microbial pesticide while ensuring the same pest preventing and controlling effect; moreover, the active ingredient camptothecin of the synergist is a plant-originated substance which is safe for people and livestock and natural enemies of the pests, can slow down the pesticide resistance of the pests to the microbial pesticide and is easy to degrade without residue; and the synergist is suitable to be used as an environmental-friendly microbial pesticide synergist and has great significance for popularization and application of the microbial pesticide.

The invention discloses a pleocidin and athomin compounded composition and application thereof.The effective ingredients of the compounded composition comprise pleocidin and athomin.A method in the technical field is adopted, the compounded composition can be prepared into emulsion in water or microemulsion or a suspending agent or wettable powder or water dispersible granules or the like.The pleocidin and athomin compounded composition has an obvious synergistic effect as a protective agent for grain storage, the insect killing toxicity is obviously higher than that of any single agent, the double effects of insect killing and mould preventing are achieved, and the insecticidal spectrum and the mould proof spectrum are wide.

The invention relates to an insecticidal composition with synergistic effect. The basic active components of the insecticidal composition are destruxins and diflubenzuron which are mixed according to a ratio of 1-9 g: 1-9 g. Adjuvants and excipients are added into the active components for preparation of a wettable powder, a suspending agent or an emulsion in water. The insecticidal composition can be applied to prevention and treatment of diamondback moth, prodenia litura, Lithocolletis ringoniella, grubs, wireworms, Trialeurodes vaporariorum, Liriomyza sativae, root maggots and Bradysia odoriphaga, preferably to prevention and treatment of lepidoptera insects like diamondback moth and prodenia litura on cruciferous vegetable, and has the advantages of stable efficacy, fast action, long action period, safety, environmental protection, etc.

The invention discloses an allyl isothiocyanate and allicin compound and an application thereof. The compound is prepared from allyl isothiocyanate (AITc), allicin and an organic solvent, and can be applied in food storage as a fumigation agent for food storage. The allyl isothiocyanate and allicin compound disclosed by the invention has an obvious synergistic effect; the insecticidal toxicity is obviously higher than that of any single dosage; the insecticidal bacteriostatic spectrum is obviously expanded; the control effect on pests, such as tribolium castaneum, oryzae philus surinamensis, sitophilus zeamais, grain moth and cryptolestes ferrugineus, in stored grain reaches over 95%; the bacteriostasis rate on a plurality of stored grain mildews such as aspergillus oryzae, aspergillus flavus, blue mould and aspergillus niger reaches over 95%; and the allyl isothiocyanate and allicin compound has relatively high cost advantages, economic benefits and relatively good control effect.

The invention discloses an insecticidal composition containing metarhizium anisopliae and cyromazine and a preparation method and application of the insecticidal composition.According to the insecticidal composition, metarhizium anisopliae and cyromazine serve as basic active ingredients, and metarhizium anisopliae conidia (piece) and cyromazine (gram) are mixed according to the ratio of (49-1) *2 *1010:(1-49).Wet powder, water dispersible granules and an oil suspending agent can be prepared after auxiliary materials are added to the active ingredients.The insecticidal composition can be used for preventing and treating pests such as scale insects, trialeurodes vaporariorum, thrips, grubs, wireworms, citrus fruit flies, liriomyza sativae, root maggots and Bradysia odoriphaga Yang et Zhang, is especially suitable for preventing and treating diptera pests such as citrus fruit flies, liriomyza sativae, root maggots and bradysia odoriphaga, and has the advantages of being stable in pesticide effect, good in fast-acting property, long in lasting period, safe, environmentally friendly and the like.

A Bacillus thuringiensis WY-190 (CCTCC No.M202005) for killing beet noctuids is prepared through loading soil sample in test tube containing nutritive liquid, vibration culture, diltuing, coating on discrete culture medium, inversion culture, purifying monoclonly, inoculating to fermenting culture medium, shake-flask fermentation until parasporal crystal is dropped, mixing with artificial feed, and biological test with target insect. Its advantage is high insecticiding toxin.

The invention belongs to the field of biotic insecticides and relates to a microbial pesticide synergist. The main ingredient of the synergist is camptothecin or a derivative thereof. The invention relates to two microbial pesticides containing the synergist at the same time, namely a microbial pesticide containing bacillus thuringiensis and the camptothecin / camptothecin derivative, and a microbial pesticide containing baculovirus and the camptothecin / camptothecin derivative. The synergist can effectively improve the pest killing effect of the bacillus thuringiensis or baculovirus pesticide, enhance the pesticide effect and reduce the dosage of the microbial pesticide while ensuring the same pest preventing and controlling effect; moreover, the active ingredient camptothecin of the synergist is a plant-originated substance which is safe for people and livestock and natural enemies of the pests, can slow down the pesticide resistance of the pests to the microbial pesticide and is easy to degrade without residue; and the synergist is suitable to be used as an environmental-friendly microbial pesticide synergist and has great significance for popularization and application of the microbial pesticide.

The invention relates to an insecticidal composition with a synergistic action. The insecticidal composition is prepared by taking destruxin and pyriproxyfen as basic active components and mixing the destruxin and the pyriproxyfen according to the following ratio of 1g to (1-99)g. After the two effective components are mixed, the insecticidal composition can be applied to preventing and controlling health pests including aedes albopictus, flies and termites and preferably preventing and controlling diptera health pests including mosquitos, flies and the like.

The invention discloses an insecticidal composition containing metarhizium anisopliae and cyromazine and a preparation method and application of the insecticidal composition.According to the insecticidal composition, metarhizium anisopliae and cyromazine serve as basic active ingredients, and metarhizium anisopliae conidia (piece) and cyromazine (gram) are mixed according to the ratio of (49-1) *2 *1010:(1-49).Wet powder, water dispersible granules and an oil suspending agent can be prepared after auxiliary materials are added to the active ingredients.The insecticidal composition can be used for preventing and treating pests such as scale insects, trialeurodes vaporariorum, thrips, grubs, wireworms, citrus fruit flies, liriomyza sativae, root maggots and Bradysia odoriphaga Yang et Zhang, is especially suitable for preventing and treating diptera pests such as citrus fruit flies, liriomyza sativae, root maggots and bradysia odoriphaga, and has the advantages of being stable in pesticide effect, good in fast-acting property, long in lasting period, safe, environmentally friendly and the like.

The invention belongs to the technical field of genetic engineering, and specifically relates to a synthetic bacillus thuringiensis insecticidal gene with virulence against lepidoptera. The synthetic gene comprises a nucleotide sequence of a Vip83Cry9Ca<*> gene and an amino acid sequence coded by the gene. Escherichia coli DH5alpha / Bt. / 6p / pGEVC9C comprising the gene plasmid is collected at China Center for Type CultureCollection (CCTCC), and has a collection number of CCTCC NO: M2011363. As a result of biological experiments, the insecticidal fusion gene has virulence against lepidoptera, and can specifically kill agricultural pests such as diamondback moth and the like. The invention also discloses an application method of the fusion protein in the respect of transgenic insecticidal microbes.

The invention relates to Cu2<+> enhanced Bacillus thuringiensis (Bt) insecticidal activity and application thereof. The Bacillus thuringiensis involved in the invention is Bacillus thuringiensisX023,a strain is preserved in China Center for Type Culture Collection on May 16, 2018, and the strain preservation number is CCTCC NO: M2018283. The application of the Cu2<+> enhanced Bacillus thuringiensis insecticidal activity comprises addition of 10<-5> M copper sulfate pentahydrate into a fermentation medium of the Bacillus thuringiensis to significantly improve the insecticidal toxicity of a fermentation bacteria solution of the Bacillus thuringiensis on cotton bollworm and plutella xylostella.

The invention discloses a pleocidin and athomin compounded composition and application thereof.The effective ingredients of the compounded composition comprise pleocidin and athomin.A method in the technical field is adopted, the compounded composition can be prepared into emulsion in water or microemulsion or a suspending agent or wettable powder or water dispersible granules or the like.The pleocidin and athomin compounded composition has an obvious synergistic effect as a protective agent for grain storage, the insect killing toxicity is obviously higher than that of any single agent, the double effects of insect killing and mould preventing are achieved, and the insecticidal spectrum and the mould proof spectrum are wide.

The present invention discloses a constructing method of insect-killing genetically engineered beauveria bassiana. Primers are designed to amplify an AaIT gene from a pUC57-AaIT vector by PCR, and a pbarGPEl-AaIT vector is constructed by digestion, purification and pbarGPEl; the primers are used to amplify a GpdA-AaIT-TrpC expression cassette; Not1 single-digestion of pGPS3Ben-bbchit1 is conducted, dephosphorylation is conducted, connection with GpdA-AaIT-TrpC digested by the Not1 is conducted, transformation screening is conducted to obtain a pGPS3Ben-bbchit1-AaIT expression vector, and the pGPS3Ben-bbchit1-AaIT expression vector is introduced into agrobacterium tumefaciens competent cells to obtain positive transformant colonies; and a beauveria bassiana spore suspension is mixed with anagrobacterium tumefaciens solution containing the pGPS3Ben-bbchit1-AaIT vector, and the transformed genetically engineered beauveria bassiana is obtained by screening a solid IM culture medium and aCPZ selection culture medium. The constructed genetically engineered beauveria bassiana can prepare a high-efficiency insect-killing biological control agent, has no pollution to the environment and also has a good prevent and cure effect, and is suitable for popularization in the technical field of biological prevention and cure.

The invention relates to an insecticidal composition with synergistic effect. The basic active components of the insecticidal composition are destruxins and diflubenzuron which are mixed according to a ratio of 1-9 g: 1-9 g. Adjuvants and excipients are added into the active components for preparation of a wettable powder, a suspending agent or an emulsion in water. The insecticidal composition can be applied to prevention and treatment of diamondback moth, prodenia litura, Lithocolletis ringoniella, grubs, wireworms, Trialeurodes vaporariorum, Liriomyza sativae, root maggots and Bradysia odoriphaga, preferably to prevention and treatment of lepidoptera insects like diamondback moth and prodenia litura on cruciferous vegetable, and has the advantages of stable efficacy, fast action, long action period, safety, environmental protection, etc.

A Bacillus thuringiensis WY-190 (CCTCC No.M202005) for killing beet noctuids is prepared through loading soil sample in test tube containing nutritive liquid, vibration culture, diltuing, coating on discrete culture medium, inversion culture, purifying monoclonly, inoculating to fermenting culture medium, shake-flask fermentation until parasporal crystal is dropped, mixing with artificial feed, and biological test with target insect. Its advantage is high insecticiding toxin.

The invention discloses a compound pesticide, which is mainly prepared from the following raw materials in percentage by weight: 5-20% of azadirachtin and metarrhizium, 8-12% of emulsifier, 3-7% of stabilizer, 2-8% of synergist, 0.1-1.0% of antioxidant, 10-18% of cosolvent and 50-60% of organic solvent. The compound pesticide is environment-friendly, has high compatibility and obvious synergisticeffect, and can obviously improve the insecticidal toxicity of a single dosage and reduce the use of high-persistent chemical pesticide.

The invention provides a subtilisin-like gene recombination insect killing engineering bacteria and the microbial inoculums preparing method thereof. The insect killing engineering bacteria is a double-effect engineering bacteria which is fused and constructed by the crylAc gene of bacillus thuringiensis and a foreign toxin gene, and expresses fused insecticidal protein crystals in the bacillus thuringiensis. The bacillus thuringiensis is the 4.0718 strain of bacillus thuringiensis. The foreign toxin gene is the CDEP2 gene of subtilisin-like of spherical spore white muscardine fungi. The titers of subtilisin-like gene recombination insect killing engineering bacteria microbial inoculums prepared by fermenting production are all above 6000 international units / microgram, the insect killing rates on the lepidoptera, dipteral and coleopteran insects all reach 90 percent. Under same consistence, the insect killing effect of the invention is 3-5 times higher than that of the monovalent microbial inoculums. The invention has no toxic residue, is safe to human being and animal, and is conducive to protecting entironment.

The invention discloses a metarhizium anisopliae transgenic strain as well as a preparation method and an application thereof. The strain is a recombinant engineering bacterium constructed by fusion of metarhizium anisopliae genes and exogenous Prla (subtilisin-like protease A) genes. The metarhizium anisopliae transgenic strain takes metarhizium anisopliae as the basis, takes Pgpd as a strong promoter, takes TTrpC as a terminator and takes Basta as a selection marker, a Prla overexpression box is constructed with a PCR fusion method, then a fungal protoplast is prepared, genetic engineering transformation is conducted with a CaCl2-PEG method, and the overexpression box is inserted in a metarhizium anisopliae gene group randomly for overexpression. The metarhizium anisopliae transgenic strain is good in German cockroach killing effect, higher in toxicity and capable of effectively controlling German cockroach populations in a short time.

The invention provides a subtilisin-like gene recombination insect killing engineering bacteria and the microbial inoculums preparing method thereof. The insect killing engineering bacteria is a double-effect engineering bacteria which is fused and constructed by the crylAc gene of bacillus thuringiensis and a foreign toxin gene, and expresses fused insecticidal protein crystals in the bacillus thuringiensis. The bacillus thuringiensis is the 4.0718 strain of bacillus thuringiensis. The foreign toxin gene is the CDEP2 gene of subtilisin-like of spherical spore white muscardine fungi. The titers of subtilisin-like gene recombination insect killing engineering bacteria microbial inoculums prepared by fermenting production are all above 6000 international units / microgram, the insect killing rates on the lepidoptera, dipteral and coleopteran insects all reach 90 percent. Under same consistence, the insect killing effect of the invention is 3-5 times higher than that of the monovalent microbial inoculums. The invention has no toxic residue, is safe to human being and animal, and is conducive to protecting entironment.

The invention discloses an insecticide for corn seedlings. The insecticide takes celastrus angulatus extract, garden balsam stem extract and pyrethroid as main components and is specifically preparedfrom the following components in parts by weight: 3-5 parts of celastrus angulatus extract, 15-22 parts of garden balsam stem extract, 3.5-4.6 parts of pyrethroid, 0.2-1.3 parts of xanthan gum, 0.6-2.6 parts of Tween-20 and 52-64 parts of water. The insecticide disclosed by the invention has the beneficial effects that all components can exert mutually synergistic effects; the insecticide is broadin spectrum, high in efficiency, not liable to generate resistance to drugs, low in toxicity, easy to degrade and free of residues; the insecticide for corn seedlings has better mobility in soil layers and has higher content of haedoxan A.

The invention discloses a microcapsule preparation containing allyl isothiocyanate, a preparation method and an application. The microcapsule preparation is composed of a mixture of polyethylene glycol as a wall material and a mixture of allyl isothiocyanate and allicin as a core material, and is prepared through the following steps: 1) heating the polyethylene glycol until it melts, and keeping the constant temperature, Rapidly add the mixture of allyl isothiocyanate and allicin to the melted polyethylene glycol, and stir to form a homogeneous blend of allyl isothiocyanate, allicin and polyethylene glycol; 2) After natural cooling, grind and pulverize, and sieve to obtain the microcapsule preparation. The microcapsule preparation containing allyl isothiocyanate prepared by the present invention has high loading rate and strong synergistic effect, can effectively improve the stability of the active ingredient containing allyl isothiocyanate and prolong its duration. Efficacy, reduce toxicity, mask odor, reduce environmental pollution; it has strong cost advantage, economic benefit and good control effect.

The invention relates to a method for acquiring high-activity Cry1Ai protein mutants and the mutants, and belongs to the biotechnology field. The method for acquiring the high-activity Cry1Ai protein mutants comprises the steps that continuous multi-point mutation is performed on an amino acid sequence in a loop 2 or / and a loop 3 of a Cry protein Domain II and comprises more than 10 continuous amino acid sequence changing. The insecticidal activity of the continuous multi-point mutant ModCry1Aid-loop 2 on cotton bollworm is improved by about 13 times, the activity of the continuous multi-point mutant ModCry1Aid-loop 2 on ostrinia nubilalis is improved by near 9 times compared with a Cry1Ai protein before improvement, and the activity of the continuous multi-point mutant ModCry1Aid-loop 2 and the continuous multi-point mutant ModCry1Aid-loop 3 on rice stem borer is improved by about 5-6 times compared with the Cry1Ai protein before improvement.