32results about How to "Improve insecticidal virulence" patented technology

The invention discloses an insecticidal composition containing metarhizium anisopliae and cyromazine and a preparation method and application of the insecticidal composition.According to the insecticidal composition, metarhizium anisopliae and cyromazine serve as basic active ingredients, and metarhizium anisopliae conidia (piece) and cyromazine (gram) are mixed according to the ratio of (49-1) *2 *1010:(1-49).Wet powder, water dispersible granules and an oil suspending agent can be prepared after auxiliary materials are added to the active ingredients.The insecticidal composition can be used for preventing and treating pests such as scale insects, trialeurodes vaporariorum, thrips, grubs, wireworms, citrus fruit flies, liriomyza sativae, root maggots and Bradysia odoriphaga Yang et Zhang, is especially suitable for preventing and treating diptera pests such as citrus fruit flies, liriomyza sativae, root maggots and bradysia odoriphaga, and has the advantages of being stable in pesticide effect, good in fast-acting property, long in lasting period, safe, environmentally friendly and the like.

A diluent for fungus-type agricultural chemicals is prepared from oil (1-98% wt.%) water (1-80%) and emulsifier thorugh proportional mixing, and homogenizing or emulsifying. Its advantages are broad spectrum, high effect on improving antibacterial power of fungus-type agricultural chemicals, and low cost.

A Bacillus thuringiensis WY-190 (CCTCC No.M202005) for killing beet noctuids is prepared through loading soil sample in test tube containing nutritive liquid, vibration culture, diltuing, coating on discrete culture medium, inversion culture, purifying monoclonly, inoculating to fermenting culture medium, shake-flask fermentation until parasporal crystal is dropped, mixing with artificial feed, and biological test with target insect. Its advantage is high insecticiding toxin.

The invention discloses a metarhizium anisopliae transgenic strain as well as a preparation method and an application thereof. The strain is a recombinant engineering bacterium constructed by fusion of metarhizium anisopliae genes and exogenous Prla (subtilisin-like protease A) genes. The metarhizium anisopliae transgenic strain takes metarhizium anisopliae as the basis, takes Pgpd as a strong promoter, takes TTrpC as a terminator and takes Basta as a selection marker, a Prla overexpression box is constructed with a PCR fusion method, then a fungal protoplast is prepared, genetic engineering transformation is conducted with a CaCl2-PEG method, and the overexpression box is inserted in a metarhizium anisopliae gene group randomly for overexpression. The metarhizium anisopliae transgenic strain is good in German cockroach killing effect, higher in toxicity and capable of effectively controlling German cockroach populations in a short time.

The invention provides a subtilisin-like gene recombination insect killing engineering bacteria and the microbial inoculums preparing method thereof. The insect killing engineering bacteria is a double-effect engineering bacteria which is fused and constructed by the crylAc gene of bacillus thuringiensis and a foreign toxin gene, and expresses fused insecticidal protein crystals in the bacillus thuringiensis. The bacillus thuringiensis is the 4.0718 strain of bacillus thuringiensis. The foreign toxin gene is the CDEP2 gene of subtilisin-like of spherical spore white muscardine fungi. The titers of subtilisin-like gene recombination insect killing engineering bacteria microbial inoculums prepared by fermenting production are all above 6000 international units / microgram, the insect killing rates on the lepidoptera, dipteral and coleopteran insects all reach 90 percent. Under same consistence, the insect killing effect of the invention is 3-5 times higher than that of the monovalent microbial inoculums. The invention has no toxic residue, is safe to human being and animal, and is conducive to protecting entironment.

The invention discloses a microcapsule preparation containing allyl isothiocyanate, a preparation method and an application. The microcapsule preparation is composed of a mixture of polyethylene glycol as a wall material and a mixture of allyl isothiocyanate and allicin as a core material, and is prepared through the following steps: 1) heating the polyethylene glycol until it melts, and keeping the constant temperature, Rapidly add the mixture of allyl isothiocyanate and allicin to the melted polyethylene glycol, and stir to form a homogeneous blend of allyl isothiocyanate, allicin and polyethylene glycol; 2) After natural cooling, grind and pulverize, and sieve to obtain the microcapsule preparation. The microcapsule preparation containing allyl isothiocyanate prepared by the present invention has high loading rate and strong synergistic effect, can effectively improve the stability of the active ingredient containing allyl isothiocyanate and prolong its duration. Efficacy, reduce toxicity, mask odor, reduce environmental pollution; it has strong cost advantage, economic benefit and good control effect.