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Cu2<+> enhanced Bacillus thuringiensis (Bt) insecticidal activity and application thereof

A technology of Bacillus thuringiensis and Bacillus aureus, which is applied in the direction of insecticides, bacteria, and the use of added compounds to stimulate growth, can solve the problems of high cost of use and inconspicuous advantages, and achieve good toxicity, good application prospects, and improved The effect of insecticidal virulence

Active Publication Date: 2019-08-27
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the popularization and application of Bacillus thuringiensis (Bt) insecticide preparations in my country has no obvious advantages over chemical pesticides, mainly due to the high cost of use

Method used

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  • Cu2&lt;+&gt; enhanced Bacillus thuringiensis (Bt) insecticidal activity and application thereof
  • Cu2&lt;+&gt; enhanced Bacillus thuringiensis (Bt) insecticidal activity and application thereof
  • Cu2&lt;+&gt; enhanced Bacillus thuringiensis (Bt) insecticidal activity and application thereof

Examples

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Embodiment

[0024] (1) The Bacillus thuringiensis X023 (abbreviated as Bt X023) of the present embodiment, which was preserved on May 16, 2018 at the China Center for Type Culture Collection (CCTCC, address: Wuhan University, Wuhan, China), strain preservation The number is CCTCCNO: M 2018283.

[0025] (2) Optical microscope observation:

[0026] Specific implementation process: Take 1.5 mL of Bt X023 cultured for 48 hours (fermentation medium without copper ions), 9000 rpm, 3 min, centrifuge, remove the supernatant, wash twice with double distilled water, and resuspend the bacteria with 200 μL double distilled water For the cells, pipette 10 μL of bacterial liquid and drop it on the center of the glass slide, cover it with a cover glass, and observe the morphology of the cells under a 100 times oil microscope. figure 1 It is a phase-contrast microscope picture of bacterial strain Bt X023 cultured to 48h, which shows that the bacterial strain is a bacterium with rod-shaped spores and rho...

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Abstract

The invention relates to Cu2<+> enhanced Bacillus thuringiensis (Bt) insecticidal activity and application thereof. The Bacillus thuringiensis involved in the invention is Bacillus thuringiensisX023,a strain is preserved in China Center for Type Culture Collection on May 16, 2018, and the strain preservation number is CCTCC NO: M2018283. The application of the Cu2<+> enhanced Bacillus thuringiensis insecticidal activity comprises addition of 10<-5> M copper sulfate pentahydrate into a fermentation medium of the Bacillus thuringiensis to significantly improve the insecticidal toxicity of a fermentation bacteria solution of the Bacillus thuringiensis on cotton bollworm and plutella xylostella.

Description

technical field [0001] The present invention relates to the optimization of Bacillus thuringiensis fermentation medium, the fermentation culture of a Bacillus thuringiensis (Bacillusthuringiensis X023) and application thereof, particularly relate to Cu 2+ Application to enhance the insecticidal activity of Bacillus thuringiensis. Background technique [0002] Bacillus thuringiensis (Bt) preparation is a highly efficient, specific and eco-friendly biopesticide, which is widely used all over the world, mainly because of the insecticidal crystal proteins (ICPs) it produces. At present, the popularization and application of Bacillus thuringiensis (Bt) insecticide preparations in my country has no obvious advantages over chemical pesticides, mainly due to the high cost of use. [0003] Early studies have shown that many factors can affect the production of insecticidal crystal proteins, one of the most important factors being mineral elements. Especially trace metal ions, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20A01P7/04C12R1/07
CPCC12N1/38C12N1/20
Inventor 丁学知邓紫如刘卓林王木兰党丹丹王云峰罗莎谢俊雁夏立秋
Owner HUNAN NORMAL UNIVERSITY
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