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Fungal farm chemicals diluent

A diluent and fungus technology, applied in biocides, animal repellents, plant growth regulators, etc., can solve the problems that cannot be used as dilute powder, long germination, irregularity, etc., to shorten the time for germination or re-growth , Improve insecticidal virulence, improve spore germination rate and uniformity

Inactive Publication Date: 2005-01-26
CHONGQING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fungal pesticide oils use oil (vegetable oil or mineral oil or various mixed oils) as a diluent, and the fungal spores in the oil can only obtain water from the insect body and the environment, so the spores absorb water and germinate for a long time and germinate quickly. Irregular; oil as diluent is also more expensive
More importantly, the use of oil as a diluent is only suitable for fungal pesticides made from hydrophobic fungal spores, not fungal pesticides processed with mycelium and hydrophilic spores; and the use of oil as a diluent can only be used As an oil prepared by diluting hydrophobic fungal spores, not as a powder prepared by diluting hydrophobic fungal spores

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: After being mixed with 56% vegetable oil, 40% water, and 4% petrolatum by weight percentage, the oil-water mixture processed by a homogenizer is used to obtain the present invention.

[0021] Apply the above-mentioned fungal pesticide diluent to the spore oil of Metarhizium anisopliae strain CQ102, 12 hours after mixing, the spore germination rate increased by 34.5%; the time for the spore germination rate to reach 90% or more was shortened by more than 3 hours; Two days after locust adults (inoculated with 5×10 4 spores / locusts), a large number of Metarhizium anisopliae can be seen growing in the body fluid of locusts, see figure 2 , the double dashed arrow in the figure refers to Metarhizium anisopliae growing in body fluid.

[0022] And do not add the control group of above-mentioned fungal pesticide diluent, then be difficult to see the metarhizium anisopliae spore growth in the locust body fluid, see figure 1 .

Embodiment 2-7

[0023] Embodiment 2-7: the fungal pesticide diluent prepared by different oils in table 1, water, emulsifier ratio is mixed with the Metarhizium anisopliae oil (edible oil) of 20% by weight percentage respectively by 1: 1 After 15 hours at 28°C, dilute to 1×10 with edible oil 7 spores / ml, draw 5 μL of spore liquid and point it under the pronotum of migratory locust adults, and inoculate 5 heads for each treatment. The inoculated locusts were placed in cages with an indoor temperature of 28°C and a relative air humidity of 40-50%, and fresh wheat seedlings were regularly replaced every day. Control group I used edible oil instead of fungal pesticide diluent, and control group II directly used a concentration of 1×10 7 spores / ml water inoculation, after inoculation, take 20 μl of the body fluid of the same treated locust at different times and mix them evenly, measure the number of Metarhizium anisopliae spores on the body ball counting board, repeat 3 times to get the average ...

Embodiment 11

[0031] Embodiment 11: In order to verify that the present invention can improve the virulence of the oil agent of Metarhizium anisopliae to control migratory locusts outdoors, the inventor prepared a fungal pesticide diluent according to the ratio listed in Table 3.

[0032] Placed outdoors with a size of 1m 3(1m * 1m * 1m) cage, put into 50 4~5 ages of East Asia migratory locust gnats and potted wheat seedlings in every cage, fungal pesticide diluent mixes with 20% metrhizium anisopliae oil agent by 1: 1 ratio, Use Taishan 18 ultra-low volume mist machine to spray ultra-low volume (ULV) at a dosage of 1000ml / ha. In the control I treatment, edible oil was used instead of the fungal pesticide diluent; the blank control was not sprayed, which was used to calculate the toxicity. Investigate the number of living insects in the enclosure every day after spraying, and get LT through statistical analysis of DPS2000 software 50 , LT 90 . During the test period, the air temperature...

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Abstract

A diluent for fungus-type agricultural chemicals is prepared from oil (1-98% wt.%) water (1-80%) and emulsifier thorugh proportional mixing, and homogenizing or emulsifying. Its advantages are broad spectrum, high effect on improving antibacterial power of fungus-type agricultural chemicals, and low cost.

Description

technical field [0001] The invention relates to a biological pesticide diluent, in particular to a fungal pesticide diluent. Background technique [0002] Fungal pesticides are a class of biological pesticides with important development prospects. The principle is to use insect pathogenic fungi (entomophytes) or fungi that antagonize pathogens to control pests or pathogenic microorganisms. Different from microbial pesticides such as insecticidal bacteria, viruses and protozoa, insecticidal fungal pesticides are the only microbial pesticides with contact characteristics, and they mainly enter the body of insects through the body wall to kill insects. [0003] Fungal pesticides use living fungi to control harmful organisms. The living fungi used as fungal pesticides include two categories: spores and hyphae, with spores as the mainstay. Fungal spores are divided into hydrophilic spores and hydrophobic spores according to surface activity. For example, the spores of the insect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N25/02A01N63/04
Inventor 夏玉先王中康彭国雄殷幼平曾德玉
Owner CHONGQING UNIV
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