37results about How to "Identification results are stable" patented technology

The invention relates to a method for identifying an aphid-resistant transgenic cotton. The method comprises the following steps: planting control varieties and checked varieties in a flower pot with a cover at room temperature; carrying out artificial aphid inoculation on each control variety and each checked variety; investigating the number of cotton aphids on each plant of the cotton the third day after the artificial aphid inoculation is carried out; and taking the blade of the cotton plate most severely damaged as a standard leaf to investigate aphid harm magnitude of the control varieties and the check varieties the seventh day after the artificial aphid inoculation is carried out, and identifying the transgenic cotton variety resistant to the aphids according to the aphid hazard index and aphid hazard index deceasing rate. The method provided by the invention features good practicability and operability and stable and reliable identification result.

The invention relates to a method for rapid identification of sweet potato stem rot resistance. The method comprises the operation steps of: trimming a sweet potato stem or side branch into a stem branch with 3-4 extended leaves, and conducting pre-culture for 2-3 days under room temperature; when fibrous roots grow out of the stem branch, placing the stem branch horizontally, stabbing a small hole by a pipettor tip between two sections in the stem branch center without penetrating the stem branch, then placing the stem branch horizontally in a sealed plastic box paved with filter paper steeped by sterile water, and making the stem branch side stabbed with the small hole upward, absorbing 15-25 microliters of a 1*10<8>cfu / mL stem rot pathogenic bacterial suspension, and injecting the suspension into the small hole for inoculated culture; and after 20-24h of culture, observing the disease condition and conducting disease grade identification on the sweet potato stem branch. The method provided by the invention has the advantages of simple and fast operation, strong practicability, strong operability, and high identification result repeatability and reliability, and has potential social and economic significance for screening excellent germplasm resources resisting sweet potato stem rot.

The invention discloses a method for identifying the bacterial wilt resistance of tobacco seedlings. A water retaining agent and pearl stone are mixed uniformly at the ratio of 2:1; the mixture is disinfected and then loaded into a 6-hole cell culture board; tobacco seeds are sowed on the cell culture board, wherein each kind of tobacco seeds is sowed in one hole, and 10-20 tobacco seeds are sowed in each hole; the cell culture board is placed in an axenic culture room to culture the tobacco seeds under the conditions that the temperature environment is 25 DEG C, the illumination time is 16 hours every day, the illumination intensity is 4,000 lux and the relative humidity is 70-80 %; after emergence of seedlings, 5 seedlings are reserved in each hole uniformly; a nutrient solution is prepared from special tobacco seedling raising fertilizers, wherein the concentration of a nitrogen fertilizer reaches 100 ppm; the nutrient solution is top-dressed into the culture board; when the seedlings send forth two true leaves, 1 ml of ralstonia solanacearum suspending liquid is vaccinated into each hole of the cell culture board through a liquid-transferring gun; investigation and recordation are carried out 15 days later. The method provided by the invention is shorter in identification period, can identify 25-day-old seedlings, is not limited by seasons and has high testing stability.

The invention relates to the field of wheat disease resistance breeding and molecular biology, in particular to a molecular marker closely linked with wheat bipolaris sorokiniana black point resistant QTL and application of molecular marker. The invention provides a molecular marker for detecting whether a wheat plant has a nucleotide sequence which is shown as SEQ ID NO: 1, wherein the molecular marker is tightly linked with a black-point-resistant wheat line Youyou 1 resistance QTL. The molecular marker is a DNA fragment with the size of 249bp, which is obtained by carrying out PCR (polymerase chain reaction) amplification enzyme digestion electrophoresis separation on a primer pair shown in nucleotide sequences SEQ ID NO: 2 and SEQ ID NO: 3 by taking wheat DNA as a template, and the marker is named as BP-4B-c1. According to the method, through molecular marker detection in the seedling stage, resistance prediction and screening of the wheat to the B.sorokiniana black point can be rapidly conducted, precious scientific research time and a large amount of manpower and material resources are saved, and the identification result is stable. Therefore, the method can accurately and efficiently screen the black-point-resistant wheat strain, and the breeding process of the high-yield black-point-resistant wheat is greatly improved.

The invention provides a specific primer, a kit and an identification method for identifying traditional Chinese medicine kwangsi gecko. A lot of primers are designed based on a CYTB gene of the kwangsi gecko; the primer with species specificity is screened out through the screening study of a large amount of primers; the primer is used for identifying the traditional Chinese medicine kwangsi gecko, so that the kwangsi gecko medicinal material can be accurately separated from other adulterants, a PCR (Polymerase Chain Reaction) amplification product is only 100 to 250bp, the molecular weight is low, a lot of traditional Chinese medicine kwangsi gecko is ensured to be identified within a short time, and an identification result is accurate, stable, reliable and high in sensitivity.

The invention discloses an intelligent color development identification device and method used for traditional Chinese Medicine decoction pieces. The intelligent color development identification device comprises a first chamber, a conveying belt device used for conveying the traditional Chinese Medicine decoction pieces is arranged in the chamber, and a reagent spraying device, a cleaning device,a blow-drying device and a shooting device are arranged above the conveying belt device in sequence, wherein the reagent spraying device is used for spraying a reagent with a developing effect to thetraditional Chinese Medicine decoction pieces; the cleaning device is used for cleaning the traditional Chinese Medicine decoction pieces; the blow-drying device is used for blow-drying the traditional Chinese Medicine decoction pieces; the shooting device is sued for shooting the traditional Chinese Medicine decoction pieces; the reagent spraying device, the cleaning device, the blow-drying device and the shooting device are connected to a central control device. Reagent spraying, cleaning, blow-drying and shooting can be automatically completed, and detection can be automatically conducted according to shot pictures without interference with subjective factors; the reagent is accurately sprayed, the quantity for spray is constant, and the identification result is more stable; the spraying operation is completed in the chamber, and the health of operators is not affected.

The invention provides a specific primer for identifying donkey-sourced components in colla corii asini, a kit and an identifying method. A large quantity of primers are designed on the basis of CYT Band COX-1 genes of donkey; through screening and research on the primers, a primer with species specificity is screened; the primer with species specificity can be used for identifying donkey-sourcedcomponents in colla corii asini and accurately separating donkey from other adulterants; a PCR amplification product thereof is only 70-120 bp and molecular weight is small; identification for a large quantity of donkey-sourced components in colla corii asini in a short time can be guaranteed; an identification result is accurate, stable, reliable and highly sensitive.

The invention discloses a molecular marking primer and method for identifying Chinese coastal platycephalus indicus species indeterminate (sp) and platycephalus cultellatus. The sequence of the molecular marking primer Fish F1 is 5'-TCAACCAACCACAAAGACATTGGCAC-3', and the sequence of the molecular marking primer Fish R2 is 5'-ACTTCAGGGTGACCGAAGAATCAGAA-3'. The molecular marking method includes: extracting DNA of platycephalus indicus species indeterminate (sp) and platycephalus cultellatus, and taking the DNA of the two fish as templates for PCR amplification, performing purification and sequencing to the amplification results, and comparing the sequencing results. The molecular marking primer is proper in length, is closely complementary to the sequences of the templates, and is beneficialfor an amplification reaction. The molecular marking method is simple, the identification result is stable, and the repeatability is good. The amplification result of Chinese coastal platycephalus indicus species indeterminate (sp) is consistent, so that the primer and method show high consistency.

The invention belongs to the technical field of maize aspergillus flavus ear rot resistance, and discloses an indoor rapid identification method for maize disease resistance, wherein the method includes the steps: A, preparing an aspergillus flavus spore suspension; B, preparing samples of sterilized test maize varieties; C, inoculating each grain of each sterilized maize sample with the aspergillus flavus spore suspension, and culturing at constant temperature to obtain maize varieties infected with disease; and D, taking the disease-infected variety with the disease grade of 9 as control, determining the average disease grade of the maize varieties infected with the disease, and classifying the resistance according to the average disease grade, so as to identify the maize aspergillus flavus ear rot resistance. The method has the advantages of short identification period, low identification cost and accurate results.

The invention provides a molecular marker for identifying a common threewingnut root medicinal material, a primer pair, a PCR reagent, a kit and application of the molecular marker in identification of the common threewingnut root medicinal material. The molecular marker is in a nucleotide sequence shown in SEQ ID NO.3, and the primer pair can amplify the nucleotide sequence shown in SEQ ID NO.3. The invention further provides an identification method of the common threewingnut root medicinal material. Through the molecular marker, the primer pair, the PCR reagent, the PCR kit and the identification method, whether or not the molecular marker is amplified is detected so that the common threewingnut root medicinal material can be identified, the repeatability is high, and the defects of high subjectivity and poor repeatability of a traditional identification method are overcome.

The invention provides a specific primer, a kit and an identification method for identifying donkey-derived components. A large number of primers are designed based on CYT B and COX-1 genes of a donkey, and are screened and researched to screen out a species-specific primer; and the primer is used for identifying the donkey-derived components in a mixture to be detected. The specific primer, the kit and the identification method provided by the invention can accurately distinguish the donkey-derived components from other adulterants, have a PCR amplification product of only 50-150bp and smallmolecular weight, ensure identification of the donkey-derived components in a large number of mixtures within a short time, and can obtain accurate, stable, reliable and highly sensitive identification results.

The invention provides a method for identifying the fertilization mode of channel catfish breeding ponds. The total DNA of the channel catfish intestinal microorganisms in the culture pond is extracted, and the total DNA is used as a template to target Lactobacillus spp. Lactobacillus The primers were designed for the DNA sequence of the 16S rDNA gene of the population, and PCR amplification was performed. The method for identifying the fertilization mode of the channel catfish pond according to the invention can quickly, simply, accurately and effectively identify the fertilization mode of the channel catfish culture pond, and has the advantages of simple operation, low cost, high accuracy, stable and reliable identification results, and can solve the problem of channel catfish cultivating ponds. Difficulty in identifying fertilization methods.

The invention discloses a method for identifying the purity of 'bud' (muskmelon variety) hybrid seeds based on EST-SSR marks. According to the method, bud H (muskmelon variety) hybrids and biparental genomic DNA thereof are used as a template, through a muskmelon EST sequence published on a Gene Bank database, the design of Primer 3.0 on-line primers is utilized, and EST-SSR primers are designed. Through screening, a pair of primers of which hybrid banding patterns are biparental complementary banding patterns is obtained; through field proof tests, the primers are favorable in stability, are fit with field test results, and can perform purity identification on muskmelon hybrid varieties. According to the detection method disclosed by the invention, the work of identifying the purity of seeds can be completed within 3h, and the detection method has the advantages of being quick, low in cost, convenient to operate and the like.

The present invention provides an edible sunflower hybrid SH363 authenticity rapid detection kit which includes a primer liquid portion, a reaction liquid portion and a DNA polymerase portion which are each individually wrapped, and the primer liquid portion comprises the at least one primer of primer SR-366, primer SR-495, primer SR-1067 and primer SR-1079. The kit can detect a large number of to-be-tested sunflowers in a short time, and the detection results are accurate, stable and reliable.

The invention discloses an anti-weevil sweet potato identification method. The anti-weevil sweet potato identification method comprises the steps that 1, compatible varieties and tested varieties of sweet potato tubers are respectively arranged in weevil rearing boxes; 2, weevil adults are inoculated; 3, the open ends of the weevil rearing boxes are covered by meshy covers; 4, the harm situations of sweet potato peels and sweet potato pulp of the compatible varieties and tested varieties of sweet potato tubers are surveyed on the 18th-25th days after inoculation, and the harm levels of the sweet potato peels and the sweet potato pulp are recorded; 5, the sweet potato peel harm index, the sweet potato peel harm index reduction rate, the sweet potato pulp harm index and the sweet potato pulp harm index reduction rate are calculated; 6, the varieties of anti-weevil sweet potatoes are identified according to the sweet potato peel harm index reduction rate and the sweet potato pulp harm index reduction rate. The varieties of anti-weevil sweet potatoes can be quickly and accurately identified by means of the identification method, therefore the identified varieties of anti-weevil sweet potatoes can be reliably subjected to conventional breeding, selection and improvement by adopting a modern biotechnology and can be widely popularized and planted, and accordingly huge economic benefit is obtained.

The invention provides an exopalaemon carinicauda sex-linked marker and a sex test method. The process of the test method is as follows: the muscular tissue genome DNA of exopalaemon carinicauda is extracted, and with the extracted genome DNA as a template, PCR (Polymerase Chain Reaction) amplification reaction is carried out; the PCR amplification product is purified and sequenced; the sequencingresult is compared with the DNA sequence of the exopalaemon carinicauda sex-linked marker, the sex is female if the sequencing map is shown as miscellaneous peaks, and the sex is male if the sequencing map is a single peak and is consistent with the marker sequence. The test method disclosed by the invention is easy to operate, the requirement on samples is relatively low, and identification results are stable, and are not influenced by tissue specificity and environment. The invention can conveniently, efficiently and relatively accurately identify the genetic sexes of exopalaemon carinicauda. The invention has a good market application prospect.

The invention provides a specific primer, a kit and an identification method for identifying traditional Chinese medicine kwangsi gecko. A lot of primers are designed based on a CYTB gene of the kwangsi gecko; the primer with species specificity is screened out through the screening study of a large amount of primers; the primer is used for identifying the traditional Chinese medicine kwangsi gecko, so that the kwangsi gecko medicinal material can be accurately separated from other adulterants, a PCR (Polymerase Chain Reaction) amplification product is only 100 to 250bp, the molecular weight is low, a lot of traditional Chinese medicine kwangsi gecko is ensured to be identified within a short time, and an identification result is accurate, stable, reliable and high in sensitivity.

The invention provides a method for identifying a fertilization mode of an ictalurus punctatus culture pond, which comprises the following steps of: extracting total DNA (deoxyribonucleic acid) of intestinal microorganisms of the ictalurus punctatus in the culture pond, designing a primer aiming at a DNA sequence of 16S rDNA (ribosomal deoxyribonucleic acid) gene of a Lactobacillus population by taking the total DNA as a template, performing PCR (polymerase chain reaction) amplification, and judging that inorganic fertilizer is used in the culture pond if an amplification product does not have a 1054bp band. According to the method for identifying the fertilization mode of the ictalurus punctatus culture pond, the channel catfish pond fertilization mode can be rapidly, simply, accurately and effectively identified, the operation is simple, the cost is low, the accuracy is high, the identification result is stable and reliable, and the problem that the channel catfish fertilization mode is difficult to identify can be solved.

The invention provides a set of primers for identifying the purity of corn varieties and its application. The primer set of the present invention consists of primer pair 1, primer pair 2, primer pair 3, primer pair 4, primer pair 5, primer pair 6, primer pair 7 and primer pair 8. It is proved by experiments that the complete set of primers of the present invention has the characteristics of high heterozygosity, high variety recognition rate, high detection efficiency, low detection cost and the like, and can quickly and accurately obtain the identification result of seed purity. In the purity identification of corn single-cross varieties, there is no need to do the screening of variety purity identification primers and small sample experiments, and the set of primers is no longer limited to the identification of the purity of individual varieties, and can be applied to most varieties in China Rapid identification of purity.

The invention provides a specific primer, a probe, a test kit and a method for detecting sugarcane components. According to the invention, a large number of primers are designed on the basis of the ITS gene, rbcl gene, nsLTP gene and NDR1 / HIN1 gene of sugarcane; meanwhile, through screening research on the large number of primers, a primer and a probe with species specificity are screened out; andthe above-mentioned primer and probe can be used for detecting the sugarcane components in a to-be-detected sample, can accurately separate sugarcane from species, can reach an absolute detection sensitivity of 0.4 copies / [mu]L and a relative detection sensitivity of 0.01%, and have accurate, stable and reliable identification results.

The invention provides a specific primer, a probe, a test kit and a method for detecting sugarcane components. According to the invention, a large number of primers are designed on the basis of the ITS gene, rbcl gene, nsLTP gene and NDR1 / HIN1 gene of sugarcane; meanwhile, through screening research on the large number of primers, a primer and a probe with species specificity are screened out; andthe above-mentioned primer and probe can be used for detecting the sugarcane components in a to-be-detected sample, can accurately separate sugarcane from species, can reach an absolute detection sensitivity of 0.4 copies / [mu]L and a relative detection sensitivity of 0.01%, and have accurate, stable and reliable identification results.

The invention belongs to the technical field of ear rot resistance of corn aspergillus flavus, and discloses an indoor rapid identification method for corn disease resistance, comprising the steps of: A, preparing a suspension of aspergillus flavus spores; B, preparing sterilized corn varieties for testing Sample; C, inoculate the spore suspension of Aspergillus flavus on each grain of each sterilized corn sample for testing, and cultivate it at a constant temperature to obtain the corn variety infected with the disease; D, use the susceptible variety with an incidence level of 9 As a control, the average disease grade of each corn variety infected with the disease was determined, and the resistance was divided according to the average disease grade, so as to realize the identification of the resistance to Aspergillus flavus ear rot in corn. The invention has the characteristics of short appraisal period, low appraisal cost and accurate result.

The invention relates to the field of wheat breeding and molecular biology, in particular to a molecular marker for detecting a wheat backbone germplasm weekly 8425B specific chromosome segment and application of the molecular marker. The molecular marker is characterized in that wheat DNA is used as a template, a primer pair as shown in nucleotide sequences Z84-1B-1F and Z84-1B-1R is used for PCR amplification, a DNA fragment with the size of 207bp is obtained after electrophoretic separation is performed by polyacrylamide gel with the mass fraction of 8%, and the marker is named as ZH84-1B-1 by the applicant. The nucleotide sequence of the molecular marker ZH84-1B-1 is as shown in SEQ ID NO: 1. The molecular marker can be used for detecting whether a wheat plant has a specific 1BL / 1RS translocation fragment of the week-8425B. The molecular marker is specific to Zhou 8425B, is linked with three disease genes, can be simultaneously used for molecular marker-assisted selection of the three diseases, and can simultaneously detect whether three disease-resistant genes are introduced into a breeding material or not after one-time hybridization, so that the detection efficiency of disease-resistant breeding is improved by two times.