Specific primer for identifying donkey-sourced components in colla corii asini, kit and identifying method

Abstract

The invention provides a specific primer for identifying donkey-sourced components in colla corii asini, a kit and an identifying method. A large quantity of primers are designed on the basis of CYT Band COX-1 genes of donkey; through screening and research on the primers, a primer with species specificity is screened; the primer with species specificity can be used for identifying donkey-sourcedcomponents in colla corii asini and accurately separating donkey from other adulterants; a PCR amplification product thereof is only 70-120 bp and molecular weight is small; identification for a large quantity of donkey-sourced components in colla corii asini in a short time can be guaranteed; an identification result is accurate, stable, reliable and highly sensitive.

Description

technical field [0001] The invention belongs to the technical field of identification of traditional Chinese medicinal materials, and in particular relates to a specific primer, a kit and an identification method for identifying donkey-derived components in donkey-hide gelatin. Background technique [0002] Donkey-hide gelatin (ASINI CORII COLLA) is a solid glue made from the dry skin or fresh skin of an equine animal donkey (Equus asinm L.), which is boiled and concentrated, and can be added with an appropriate amount of rice wine, rock sugar and soybean oil. It has the functions of nourishing blood and nourishing yin. Moisturizes dryness and stops bleeding, and is used to treat symptoms such as blood deficiency and chlorosis, dizziness, palpitations, muscle flaccidity and weakness, restlessness and insomnia. Ejiao is a variety included in the 2015 edition of the "Chinese Pharmacopoeia". Its medicinal use has a long history. It is an important source species, especially ef...

AI Technical Summary

Problems solved by technology

Both of the above two patent documents identify the direct products of donkeys (donkey hide or donkey meat), which is relatively difficult and high-quality DNA can be obtained by direct extraction. However, donkey-hide gelatin is a deep-processed product. During the production of donkey-hide gelatin, donkey The skin is decocted at high temperature for a long time, and the DNA is severely damaged. The DNA content in the finished product is small and the fragments are extremely short. Moreover, the main components of the finished gelatin are various collagen peptides. The existence of a large amount of protein will seriously affect the efficiency of DNA extraction.

In addition, in the process of preparing the finished donkey-hide gelatin, it not only needs to be boiled for a long time, but also auxiliary materials such as rice wine and soybean oil are added in the later stage. The addition of these auxiliary materials will increase the difficulty of extracting the DNA of the finished donkey-hide gelatin and introduce various components that inhibit the PCR reaction. The above reasons make the identification of donkey-derived components in donkey-hide gelatin very difficult. When using the method of the above prior art to identify donkey-derived components in donkey-hide gelatin, it is easy to have problems such as amplification difficulties and miscellaneous bands, and the accuracy and sensitivity are low. Poor, are not suitable for the identification of donkey-hide gelatin

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