Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying fertilization mode of ictalurus punctatus culture pond

A channel catfish and pond technology, applied in biochemical equipment and methods, microbial determination/inspection, climate change adaptation, etc., can solve problems such as inability to identify fertilization methods, differences in labor costs, and achieve high accuracy and stable identification results. , the effect of low cost

Active Publication Date: 2021-12-14
FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in China, the cost of fertilizers and labor costs are different from those in the United States. Therefore, there are differences in the use of inorganic fertilizers and organic fertilizers in aquaculture ponds.
At present, it is impossible to identify the method of fertilization in the breeding process of channel catfish

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying fertilization mode of ictalurus punctatus culture pond
  • Method for identifying fertilization mode of ictalurus punctatus culture pond

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The test pond was 1.5 mu with 9 wells, divided into 3 groups with organic fertilizer, inorganic fertilizer and no fertilization, with 3 parallels in each group. Before the start of pond culture, drain the pond, sprinkle strong chlorine essence on the clear pond, and after killing wild fish, add water to 1.2m. A week later, on May 30, fertilization began. The inorganic fertilizer group applied urea (Jiangsu Linggu Chemical Group Co., Ltd., TN≥46%) and compound fertilizer (Henan Poly Fertilizer Co., Ltd., N:P) according to 20kg / ha N and 2kg / ha P 2 o 5 :K 2 O=16:16:16); the organic fertilizer group is applied multifunctional biological type 1 aquatic product special fertilizer (Jiangsu Lihua Biological Fertilizer Co., Ltd., total nutrient ≥ 5%, organic matter ≥ 54%), and 120kg is applied in every pond according to the dosage instructions . After the fertilizer granules are dissolved in water, the whole pond is evenly sprinkled, and the dosage is halved in the 2nd to 4t...

Embodiment 2

[0029] Collect 20 channel catfish with organic fertilizers from Pukou and Yangzhong channel catfish breeding ponds in Jiangsu, and 60 channel catfish with inorganic fertilizers from Dafeng, Jianhu, Xiangshui, Dongtai, Haian, Rudong and other places channel catfish breeding ponds . Before the breeding starts, fertilize according to conventional breeding management. The organic fertilizer used is bagged organic fertilizer granules produced by regular manufacturers sold in the market, and the inorganic fertilizer is commercially available agricultural conventional bagged urea and compound fertilizer. The start time of breeding in sampling ponds ranges from mid-to-late March to mid-April, and the initial stocking weight is about 50g. The sampling time is from May to mid-July, 2-3 months after fertilization, and the average body weight is 267g.

[0030] The intestinal content of each channel catfish was collected at the pond mouth, put into a centrifuge tube for quick freezing in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for identifying a fertilization mode of an ictalurus punctatus culture pond, which comprises the following steps of: extracting total DNA (deoxyribonucleic acid) of intestinal microorganisms of the ictalurus punctatus in the culture pond, designing a primer aiming at a DNA sequence of 16S rDNA (ribosomal deoxyribonucleic acid) gene of a Lactobacillus population by taking the total DNA as a template, performing PCR (polymerase chain reaction) amplification, and judging that inorganic fertilizer is used in the culture pond if an amplification product does not have a 1054bp band. According to the method for identifying the fertilization mode of the ictalurus punctatus culture pond, the channel catfish pond fertilization mode can be rapidly, simply, accurately and effectively identified, the operation is simple, the cost is low, the accuracy is high, the identification result is stable and reliable, and the problem that the channel catfish fertilization mode is difficult to identify can be solved.

Description

technical field [0001] The invention relates to the technical field of identification of aquatic organisms, in particular to a method for identifying fertilization methods in channel catfish culture ponds. Background technique [0002] Channel catfish, also known as channel catfish, is native to North America and is the most important freshwater farmed fish in the United States. It has fresh meat, strong disease resistance, rapid growth, easy to catch, and wide temperature adaptability. After being introduced to my country in the 1980s, the industry It has developed rapidly, and the annual output has exceeded 300,000 tons. After continuous improvement and innovation in my country's aquaculture industry, my country's channel catfish breeding scale and unit breeding output have fully surpassed the United States, achieving better economic benefits. The first of the aquaculture horoscopes summed up by our country's aquatic science and technology workers is to emphasize water, and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/686C12Q1/6888
CPCC12Q1/686C12Q1/6888C12Q2600/124C12Q2565/125Y02A40/81
Inventor 钟立强王明华张世勇姜虎成陈校辉
Owner FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com