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Indoor rapid identification method for maize disease resistance

An identification method, corn technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems that the identification results are greatly affected by climate conditions, heavy workload, high cost, etc., achieve stable identification results and avoid land use The cost, the effect that the result is accurate

Active Publication Date: 2019-04-30
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the existing corn Aspergillus flavus ear rot resistance identification technology is generally field artificial inoculation technology. Under the condition of planting corn varieties in the field, the corn ear is inoculated with a fungus-carrying needle during the silking stage, until the ear is mature. The resistance level survey is carried out for identification when harvesting; the defect of the existing technology is that corn varieties need to be planted in the field, the workload is high, the cost is high, and the cycle is long, and the identification results are greatly affected by climate conditions

Method used

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  • Indoor rapid identification method for maize disease resistance

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Embodiment 1

[0028] A kind of indoor quick identification method of corn disease resistance, comprises steps: A, preparation flavus spore suspension; B, the maize variety sample that prepares disinfection; C, inoculate a flavus spore suspension in each On each kernel of the tested corn sample of disinfection, constant temperature culture, obtain the corn variety that infects disease; D, use the susceptible variety that disease level is 9 grades as contrast, determine the average disease level of the corn variety of every kind of infection disease, The resistance is divided according to the average disease grade, so as to realize the identification of the resistance to Aspergillus flavus ear rot in corn.

Embodiment 2

[0030] A kind of indoor quick identification method of corn disease resistance, comprises steps: A, preparation flavus spore suspension; B, the maize variety sample that prepares disinfection; C, inoculate a flavus spore suspension in each On each kernel of the tested corn sample of disinfection, constant temperature culture, obtain the corn variety that infects disease; D, use the susceptible variety that disease level is 9 grades as contrast, determine the average disease level of the corn variety of every kind of infection disease, The resistance is divided according to the average disease grade, so as to realize the identification of the resistance to Aspergillus flavus ear rot in corn.

[0031] The step A includes: inoculating the Aspergillus flavus species on the PDA plate, cultivating the Aspergillus flavus spores at a constant temperature, and mixing the Aspergillus flavus spores with sterile water to obtain the Aspergillus flavus spore suspension.

[0032] PDA among t...

Embodiment 3

[0034] A kind of indoor quick identification method of corn disease resistance, comprises steps: A, preparation flavus spore suspension; B, the maize variety sample that prepares disinfection; C, inoculate a flavus spore suspension in each On each kernel of the tested corn sample of disinfection, constant temperature culture, obtain the corn variety that infects disease; D, use the susceptible variety that disease level is 9 grades as contrast, determine the average disease level of the corn variety of every kind of infection disease, The resistance is divided according to the average disease grade, so as to realize the identification of the resistance to Aspergillus flavus ear rot in corn.

[0035] The step A includes: inoculating the Aspergillus flavus species on the PDA plate, cultivating the Aspergillus flavus spores at a constant temperature, and mixing the Aspergillus flavus spores with sterile water to obtain the Aspergillus flavus spore suspension.

[0036] PDA among t...

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Abstract

The invention belongs to the technical field of maize aspergillus flavus ear rot resistance, and discloses an indoor rapid identification method for maize disease resistance, wherein the method includes the steps: A, preparing an aspergillus flavus spore suspension; B, preparing samples of sterilized test maize varieties; C, inoculating each grain of each sterilized maize sample with the aspergillus flavus spore suspension, and culturing at constant temperature to obtain maize varieties infected with disease; and D, taking the disease-infected variety with the disease grade of 9 as control, determining the average disease grade of the maize varieties infected with the disease, and classifying the resistance according to the average disease grade, so as to identify the maize aspergillus flavus ear rot resistance. The method has the advantages of short identification period, low identification cost and accurate results.

Description

technical field [0001] The invention belongs to the technical field of corn aspergillus flavus ear rot resistance identification, and in particular relates to an indoor rapid identification method for corn disease resistance. Background technique [0002] Corn ear rot is a corn disease closely related to human health. Occurs in corn-producing regions around the world. The fungal complex of this disease produces several mycotoxins, such as aflatoxins and fumonisins, chemicals that affect human and animal health. [0003] Aspergillus flavus (Aflavus) in Aspergillus not only harms various grains such as corn, but also produces a toxic metabolite aflatoxin, which causes poisoning of humans, livestock and poultry. Medical research shows that long-term consumption of foods containing low concentrations of aflatoxin is considered to be the main cause of liver cancer, gastric cancer, intestinal cancer and other diseases. [0004] Both corn ears and grains can be damaged. The symp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 邹成佳李晓杨晓蓉崔丽娜章振羽张海燕向运佳郑双丽杨芳英
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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