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A rapid indoor identification method for maize disease resistance

An identification method and corn technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems that identification results are greatly affected by climatic conditions, heavy workload and high cost, and achieve stable identification results and avoid land use. Cost, accurate results

Active Publication Date: 2022-05-06
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0006] However, the existing corn Aspergillus flavus ear rot resistance identification technology is generally field artificial inoculation technology. Under the condition of planting corn varieties in the field, the corn ear is inoculated with a fungus-carrying needle during the silking stage, until the ear is mature. The resistance level survey is carried out for identification when harvesting; the defect of the existing technology is that corn varieties need to be planted in the field, the workload is high, the cost is high, and the cycle is long, and the identification results are greatly affected by climate conditions

Method used

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  • A rapid indoor identification method for maize disease resistance
  • A rapid indoor identification method for maize disease resistance

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Embodiment 1

[0028] A kind of indoor quick identification method of corn disease resistance, comprises steps: A, preparation flavus spore suspension; B, the maize variety sample that prepares disinfection; C, inoculate a flavus spore suspension in each On each kernel of the tested corn sample of disinfection, constant temperature culture, obtain the corn variety that infects disease; D, use the susceptible variety that disease level is 9 grades as contrast, determine the average disease level of the corn variety of every kind of infection disease, The resistance is divided according to the average disease grade, so as to realize the identification of the resistance to Aspergillus flavus ear rot in corn.

Embodiment 2

[0030] A kind of indoor quick identification method of corn disease resistance, comprises steps: A, preparation flavus spore suspension; B, the maize variety sample that prepares disinfection; C, inoculate a flavus spore suspension in each On each kernel of the tested corn sample of disinfection, constant temperature culture, obtain the corn variety that infects disease; D, use the susceptible variety that disease level is 9 grades as contrast, determine the average disease level of the corn variety of every kind of infection disease, The resistance is divided according to the average disease grade, so as to realize the identification of the resistance to Aspergillus flavus ear rot in corn.

[0031] The step A includes: inoculating the Aspergillus flavus species on the PDA plate, cultivating the Aspergillus flavus spores at a constant temperature, and mixing the Aspergillus flavus spores with sterile water to obtain the Aspergillus flavus spore suspension.

[0032] PDA among t...

Embodiment 3

[0034] A kind of indoor quick identification method of corn disease resistance, comprises steps: A, preparation flavus spore suspension; B, the maize variety sample that prepares disinfection; C, inoculate a flavus spore suspension in each On each kernel of the tested corn sample of disinfection, constant temperature culture, obtain the corn variety that infects disease; D, use the susceptible variety that disease level is 9 grades as contrast, determine the average disease level of the corn variety of every kind of infection disease, The resistance is divided according to the average disease grade, so as to realize the identification of the resistance to Aspergillus flavus ear rot in corn.

[0035] The step A includes: inoculating the Aspergillus flavus species on the PDA plate, cultivating the Aspergillus flavus spores at a constant temperature, and mixing the Aspergillus flavus spores with sterile water to obtain the Aspergillus flavus spore suspension.

[0036] PDA among t...

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Abstract

The invention belongs to the technical field of ear rot resistance of corn aspergillus flavus, and discloses an indoor rapid identification method for corn disease resistance, comprising the steps of: A, preparing a suspension of aspergillus flavus spores; B, preparing sterilized corn varieties for testing Sample; C, inoculate the spore suspension of Aspergillus flavus on each grain of each sterilized corn sample for testing, and cultivate it at a constant temperature to obtain the corn variety infected with the disease; D, use the susceptible variety with an incidence level of 9 As a control, the average disease grade of each corn variety infected with the disease was determined, and the resistance was divided according to the average disease grade, so as to realize the identification of the resistance to Aspergillus flavus ear rot in corn. The invention has the characteristics of short appraisal period, low appraisal cost and accurate result.

Description

technical field [0001] The invention belongs to the technical field of corn aspergillus flavus ear rot resistance identification, and in particular relates to an indoor rapid identification method for corn disease resistance. Background technique [0002] Corn ear rot is a corn disease closely related to human health. Occurs in corn-producing regions around the world. The fungal complex of this disease produces several mycotoxins, such as aflatoxins and fumonisins, chemicals that affect human and animal health. [0003] Aspergillus flavus (Aflavus) in Aspergillus not only harms various grains such as corn, but also produces a toxic metabolite aflatoxin, which causes poisoning of humans, livestock and poultry. Medical research shows that long-term consumption of foods containing low concentrations of aflatoxin is considered to be the main cause of liver cancer, gastric cancer, intestinal cancer and other diseases. [0004] Both corn ears and grains can be damaged. The symp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 邹成佳李晓杨晓蓉崔丽娜章振羽张海燕向运佳郑双丽杨芳英
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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