Method for identifying bacterial wilt resistance of tobacco seedlings

Abstract

The invention discloses a method for identifying the bacterial wilt resistance of tobacco seedlings. A water retaining agent and pearl stone are mixed uniformly at the ratio of 2:1; the mixture is disinfected and then loaded into a 6-hole cell culture board; tobacco seeds are sowed on the cell culture board, wherein each kind of tobacco seeds is sowed in one hole, and 10-20 tobacco seeds are sowed in each hole; the cell culture board is placed in an axenic culture room to culture the tobacco seeds under the conditions that the temperature environment is 25 DEG C, the illumination time is 16 hours every day, the illumination intensity is 4,000 lux and the relative humidity is 70-80 %; after emergence of seedlings, 5 seedlings are reserved in each hole uniformly; a nutrient solution is prepared from special tobacco seedling raising fertilizers, wherein the concentration of a nitrogen fertilizer reaches 100 ppm; the nutrient solution is top-dressed into the culture board; when the seedlings send forth two true leaves, 1 ml of ralstonia solanacearum suspending liquid is vaccinated into each hole of the cell culture board through a liquid-transferring gun; investigation and recordation are carried out 15 days later. The method provided by the invention is shorter in identification period, can identify 25-day-old seedlings, is not limited by seasons and has high testing stability.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance identification, in particular to a method for identifying tobacco bacterial wilt resistance at the seedling stage. Background technique [0002] A tobacco disease caused by bacteria has occurred in many smoking areas in my country and is seriously harmful. There is no specific control agent for bacterial wilt, and once it occurs, it is difficult to control it. In areas prone to bacterial wilt, the most important method is to use tobacco varieties resistant to bacterial wilt. The identification of bacterial wilt disease resistance of tobacco varieties mainly relies on the identification of field disease nurseries, but the identification of field disease nurseries has the disadvantages of long identification time, high cost, great influence of soil fertility and season, and unstable resistance identification. Chinese patent CN 200910094174 "A Seedling Identification Method for Tob...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention: Aiming at the problems in the prior art that the resistance identification is unstable, the identification period is long, and it is greatly affected by soil fertility and seasons, the present invention provides a method for identifying tobacco bacterial wilt resistance at the seedling stage. It is a unique method, which has the advantages of short identification cycle, no seasonal limit and stable identification results.