The invention discloses a kidney preservation bag in kidney transplantation, which comprises a heat preservation box, a sealing cover, a moving mechanism, an exhaust mechanism, a containing bag, avoiding holes, a self-sealing zipper and a hanging belt. According to the kidney preservation bag in kidney transplantation, Firstly, the device can carry out isolated storage treatment on an ice bag anda kidney needing to be transplanted, the ice bag is subjected to heat preservation treatment in a closed environment, and the medical personnel discharge cold air of the ice bag to the kidney area byusing the moving mechanism of the device, so that the kidney is refrigerated and stored. Therefore, the device can prevent the ice bag from being in direct contact with the kidney, the kidney is protected, and the operation effect is ensured.