The invention discloses a bee pupa worm health care wine, queen bee embryo or drone pupa is taken as an only raw material, low temperature processing technologies such as refrigeration, vacuum degassing, compound enzyme biological enzymolysis, vacuum centrifugation, bio-demulsification, ultrasonic treatment and high voltage pulse electric field treatment are adopted in the whole process, the utilization rate of the queen bee embryo or drone pupa is effectively improved, and loss of nutrition and health care substances is reduced. Ultrasonic waves and peracetic acid are organically combined to accelerate aging of base wine, the base wine is scientifically compounded with queen bee embryo or drone pupa hydrolysate, high voltage pulse aging accelerating is used again, oxidation of peracetic acid to bioactive components of the queen bee embryo hydrolysate is prevented, the aging effect of the queen bee embryo wine is improved, and the taste, the stability and the clarity of the queen bee embryo or drone pupa wine are improved. Finally the queen bee embryo or drone pupa health care wine which is free of worm fishy smell, high in stability, coordinated in taste, mellow and thick, soft, lasting, appropriate is flavor, high in appropriate and nutritive value, good in health care efficacy, clear and transparent is prepared.