139 results about "Transplanted Organs" patented technology

Compositions and methods are described for highly sensitive quantification of the relative representation of DNA from adaptive immune cells (e.g., T and/or B lymphocytes) in DNA extracted from complex mixtures of cells that include cells which are not adaptive immune cells. Included are methods for determining the relative presence in a tumor of tumor infiltrating lymphocytes (TIL), the relative presence of lymphocytes infiltrating a somatic tissue that is the target of an autoimmune disease, and the relative presence of lymphocytes infiltrating a transplanted organ.

The invention discloses a preparation and purification method of an antibody for inhibiting a chronic rejection of a transplanted organ. The preparation and purification method comprises the following steps of: step 1, collecting a periphery blood mononuclear cell through a lymphocyte separating medium and carrying out induction culture with 20ng/ml MICA; step 2, extracting a mononuclear cell protein through a nuclear protein and cytoplasm protein extraction kit; step 3, carrying out series connection on a high performance liquid chromatography (HPLC) purification anti-MICA antibody; and step 4, identifying the anti-MICA antibody and using rMICA and WesternBlot to carry out a reverse authentication. The anti-MICA antibody prepared through the method can inhibit a genetic expression of an endogenous MICA by sealing an endogenous MICA protein, an endogenous MICA and an siRNA, and therefore, an expression effect of an MICA of a transplanted organ is blocked, namely, expressions of an MICA gene and an MICA protein in a donator organ after an organ transplantation operation are effectively reduced.