30 results about "Protein overexpression" patented technology

The present invention relates to small molecule antagonists of Bcl-2 family proteins such as Bcl-2 and / or Bcl-XL. In particular, the present invention provides non-peptide cell permeable small molecules (e.g., tricyclo-dibenzo-diazocine-dioxides) that bind to a pocket in Bcl-2 / Bcl-XL that block the anti-apoptotic function of these proteins in cancer cells and tumor tissues exhibiting Bcl-2 protein overexpression. In preferred embodiments, the small molecules of the present invention are active at the BH3 binding pocket of Bcl-2 family proteins (e.g., Bcl-2, Bcl-XL, and Mcl-1). The compositions and methods of the present invention are useful therapeutics for cancerous diseases either alone or in combination with chemotherapeutic or other drugs.

The present disclosure relates to methods for the early detection of ERG over-expression, gene rearrangements, and diagnosis and / or prognosis of prostate cancer using a combined ERG IHC-FISH assay.

The present invention relates to small molecule antagonists of Bcl-2 family proteins such as Bcl-2 and / or Bcl-XL. In particular, the present invention provides non-peptide cell permeable small molecules (e.g., tricyclo-dibenzo-diazocine-dioxides) that bind to a pocket in Bcl-2 / Bcl-XL that block the anti-apoptotic function of these proteins in cancer cells and tumor tissues exhibiting Bcl-2 protein overexpression. In preferred embodiments, the small molecules of the present invention are active at the BH3 binding pocket of Bcl-2 family proteins (e.g., Bcl-2, Bcl-XL, and Mcl-1). The compositions and methods of the present invention are useful therapeutics for cancerous diseases either alone or in combination with chemotherapeutic or other drugs.

The invention belongs to the field of clinical prognosis in molecular biology and relates to an application of quantitative detection of a CPT1A gene or protein to prognosis of esophageal squamous cell carcinomas, and in particular relates to an application of a quantitative detection reagent of a CPT1A gene or protein in preparation of a prognosis kit of esophageal squamous cell carcinomas, a kit for prognosis of esophageal squamous cell carcinomas and a method for detecting CPT1A gene copy numbers or protein expression levels of esophageal squamous cell carcinomas patients. CPT1A gene copy number increase or protein overexpression prompts that the postoperative life cycles of the esophageal squamous cell carcinomas patients are short.

The invention discloses an alpha lipoic acid biosynthesis method, an engineered strain and a preparation method thereof. The biosynthesis method is as below: cloning an Escherichia coli lipD gene, an lplA gene, a metK gene and a lipA lipoic acid synthase gene; constructing a lipoic acid structural domain protein overexpression vector; cloning lplA into the vector highly expressing structural domain protein in series way structure to ensure full caprylyl; constructing a lipA expression vector or expression vector in series connection with metK; transforming the 4 vectors into appropriate Escherichia coli strains, regulating and comparing the medium composition and culture conditions, and promoting the conversion of caprylyl structural domain protein to lipoyl. The preparation method of alpha-lipoic acid provided by the invention employs combined low value chemical raw materials and pure biological production method, and has the advantages of little toxic substances in the production process, less pollution to the environment, high safety of the synthesized lipoic acid and high yield; and the alpha-lipoic acid has high activity.

The invention provides an XNDL2 gene, a protein, an overexpression vector, an acquisition method for rice resistant to rice sheath blight disease and application of the XNDL2 gene, belonging to the technical field of biology. The nucleotide sequence of the XNDL2 gene is shown as SEQ ID No. 1. The XNDL2 gene provided by the invention has the effect of resisting rice sheath blight disease. When theXNDL2 gene is introduced into rice, the rice capable of resisting rice sheath blight disease can be obtained.

A method for treating cancer comprising identifying a mammal that overexpresses breast cancer resistance protein; and administering to said mammal a pharmaceutical composition comprising a therapeutically effective amount of ixabepilone. In one aspect, the mammal is not administered an agent that is susceptible to breast cancer resistance protein overexpression resistance. In another aspect, the cancer is a solid tumor.

The invention relates to a medical application of recombinant protein Semaphorin3G in prevention and treatment of retinal diseases. According to the invention, the level of Sema3G protein in the retina is improved through adeno-associated virus mediated Sema3G protein overexpression in retinal vascular endothelial cells or injection of Sema3G recombinant protein into vitreous cavities, the pathological process of the OIR mouse retinopathy can be effectively improved, and the Sema3G protein can reduce the area of a vascular occlusion area in the retinopathy, and can also reduce the area of pathological neovascularization clusters, and relieve pathological phenotypes.

The present disclosure relates to methods for the early detection of ERG over-expression, gene rearrangements, and diagnosis and / or prognosis of prostate cancer using a combined ERG IHC-FISH assay.

The invention discloses preparation and applications for mycobacterium tuberculosis PUP protein overexpression strains. BCG strains are cultivated, and the genome DNAs thereof are extracted. The genome DNAs of the BCG strains are taken as a template, and the PCR amplification of a mycobacterium tuberculosis Pup protein gene can be achieved; and sequencing and identification is performed. Escherichia coli-mycobacterium tuberculosis shuttle plasmid pMV361 is taken as a carrier, and recombinant shuttle plasmid pMV361-Pup : BCG is constructed and identified. An electroporation technology is utilized to transfer the constructed escherichia coli-mycobacterium tuberculosis shuttle plasmid pMV361 carrier (recombinant shuttle plasmid pMV361-Pup : BCG) into the BCG strains to construct and identifymycobacterium tuberculosis Pup protein overexpression strains which can be named as BCG : Pup strains. The strains can be used for studying the effects of ubiquitin-like proteasome system related protein PUP of mycobacterium tuberculosis on growth, drug resistance, permeability regulation, acid resistance and pathogenicity in hosts, and can also be used for the development and research of novel vaccines for intervening in and treating tuberculosis.

The invention discloses a method for inhibiting geminivirus infection by overexpression of arabidopsis ABI5 protein, which inhibits transcription of geminivirus coding genes by overexpression of plant transcription factor ABI5 protein and combination of ABI5 protein and a promoter of geminivirus through specificity, and realizes improvement of virus resistance of plants.

The invention provides a recombinant vector for promoting BNP (Brain Natriuretic Peptide) protein overexpression. The recombinant vector for promoting the BNP protein overexpression belongs to the field of biotechnology, and comprises a BNP coding sequence and a pAAV-TRE3G-P2A-GFP vector. The pAAV-TRE3G-P2A-GFP vector comprises AsiSI and MluI restriction enzyme cutting sites; and the BNP sequence is forward inserted into the restriction enzyme cutting sites. The recombinant vector for promoting the BNP protein overexpression provided by the invention has the advantages of high transfection efficiency, capability of promoting the BNP protein overexpression and continuously stable expression, and is simple in construction method.

The present application relates to a pharmaceutical composition containing compound 5-fluoro-3-phenyl-2-[(1S)-1-(7H-purin-6-ylamino)propyl]-4-3H)-quinazolinone, and a pharmaceutically acceptable saltthereof for preventing and treating liver cancer, and the composition of the present application inhibits ROS-PI3K-AKT-TERT signal transduction, and thus can be favorably used in the treatment of a liver cell cancer, in which a protein involved in the signal transduction is overexpressed, especially, advanced liver cell cancer.

Provided is a ramp tag capable of solving instability in translation rate resulting from poor compatibility between codons in a foreign gene and a host when expressing a recombinant protein in E. coli. Unlike the conventional codon optimization or codon deoptimization method for solving the problem of rare codons, the present invention increases an expression efficiency of a target protein by merely having the ramp tag be fused with a target gene or independently expressed, without changing the original codon sequence, thereby allowing tRNA to be reused. Thus, the present invention provides a novel method for increasing recombinant protein expression which is capable of reducing costs and time in comparison to the codon optimization method that artificially synthesizes DNA sequences. Therefore, it is expected that the method of the present invention will be able to be used in production of high value-added pharmaceuticals or industrial enzymes.

A novel polypeptide which is useful in screening of an agent for improving insulin resistance and an agent for improving glucose metabolism, a polynucleotide encoding the polypeptide, an expression vector comprising the polynucleotide, and a cell transformed with the expression vector are disclosed. The polypeptide is a protein expressed in skeletal muscle. When the protein is overexpressed, incorporation of sugar into a cell is inhibited. A method for screening of an agent for improving insulin resistance and / or an agent for improving glucose metabolism in which the polypeptide is used, and a method for producing a pharmaceutical composition for insulin resistance and / or glucose metabolism improvement comprising a substance obtained according to the method for screening as an active ingredient are also disclosed.

The invention discloses a method for improving the organic selenium synthesis ability of microorganisms based on sulfur-containing protein overexpression , and belongs to the technical field of biosynthesis of organic selenium. An exogenous sulfur-containing protein gene is introduced into a microbial genome, and the obtained genome is cultured in a high-selenium and low-sulfur environment. Sulfurin sulfur-containing amino acids (cysteine and methionine) is replaced by selenium during the overexpression of an exogenous sulfur-containing protein to synthesize selenosulfate amino acid in order to synthesize a selenoprotein, so efficient organic selenium synthesis of the microorganisms is achieved. The method is based on a substitution competition relationship between selenium and sulfur,a process for synthesizing the organic selenium by using the selenoprotein is provided, the sulfur-containing foreign protein is constructed in a biotechnical way, and the selenoprotein that is the organic selenium is formed in transferred microorganisms. The method can efficiently enrich selenium and ensure the safety.

The invention discloses a method for preparing glucaric acid through whole-cell transformation, and belongs to the technical field of biology. Escherichia coli is used as a production strain to heterologously express glucuronic acid dehydrogenase from different sources, escherichia coli cells with protein overexpression are obtained through high-density fermentation culture, and glucuronic acid isused as a substrate to produce glucaric acid. A conversion liquid has the advantages of single component, short production period, simple operation, low production cost, no potential safety hazard and the like.

The invention discloses application of branched aminotransferase 1 and / or branched aminotransferase 2. The invention for the first time finds that expression of branched aminotransferase 1 (BCAT1) andbranched aminotransferase 2 (BCAT2) in the cardiac tissue of an acute myocardial ischemia model mouse is abnormally reduced, and the phenomenon of abnormal reduction of expression also occurs in an oxygen-glucose deprivation induced myocardial injury cell model. Cardiac in-situ BCAT1 and BCAT2 protein overexpression is carried out on the mouse respectively, thus finding that myocardial ischemia injury can be effectively relieved. Therefore, the BCAT1 and the BCAT2 are proved to be able to serve as novel treatment targets for screening drugs for preventing, relieving and / or treating myocardialischemia injury.

The invention discloses a biosynthesis method of α-lipoic acid, an engineering strain and a preparation method thereof. The biosynthesis method is as follows: cloning Escherichia coli lipD gene, lplA gene, metK gene and lipA lipoic acid synthase gene; constructing lipoic acid synthase gene Overexpression vector of octanoic acid domain protein; tandem cloning of lplA into a vector for high-efficiency expression domain protein to ensure complete octanoylation of domain protein; construction of lipA or tandem expression vector with metK; transform the above four vectors into suitable In Escherichia coli strains, media composition and culture conditions were adjusted and compared to promote the conversion of octanoylation domain proteins to lipoylation. The α-lipoic acid preparation method of the present invention has the advantages of combining low-value chemical raw materials with pure biological production methods, less toxic substances in the production process, and less environmental pollution. Its synthetic lipoic acid has high safety and high yield; the obtained α-lipoic acid activity is high.

The invention provides hybridoma cell strains capable of secreting anti-Spondin1 monoclonal antibody, and the preservation numbers of the hybridoma cell strains are CCTCC NO: C202093 and CCTCC NO: C202094 respectively. The invention also provides a monoclonal antibody secreted by the hybridoma cell strain secreting the anti-Spondin1 monoclonal antibody. The invention provides a test kit containingthe monoclonal antibody. The hybridoma cell strains capable of secreting anti-Spondin1 monoclonal antibody are obtained, two monoclonal antibody aiming at different epitopes of the Spondin1 are obtained, the specificity is high, the sensitivity is high, the content of the human bottom plate reactive protein Spondin1 in body fluid and tissue can be detected through pairing, and the invention is applied to detection of Spondin1 protein overexpression or diagnosis of products of diseases characterized by abnormal expression of Spondin1 protein.

Provided is a ramp tag capable of solving instability in translation rate resulting from poor compatibility between codons in a foreign gene and a host when expressing a recombinant protein in E. coli. Unlike the conventional codon optimization or codon deoptimization method for solving the problem of rare codons, the present invention increases an expression efficiency of a target protein by merely having the ramp tag be fused with a target gene or independently expressed, without changing the original codon sequence, thereby allowing tRNA to be reused. Thus, the present invention provides a novel method for increasing recombinant protein expression which is capable of reducing costs and time in comparison to the codon optimization method that artificially synthesizes DNA sequences. Therefore, it is expected that the method of the present invention will be able to be used in production of high value-added pharmaceuticals or industrial enzymes.

There are provided new heterobifunctional agents designed to mediate formation of protein-protein dimers and promote ubiquitination of a protein of interest component of the dimer. Also provided are methods of synthesizing the agents, pharmaceutical formulations including the agents, and methods of using the agents to treat, ameliorate or cure diseases characterized by protein over-expression or malfunction.

The present invention relates to expression vectors containing in vivo urea cycle enzyme gene; transformants thereof; and the use of transformants for protein overexpression. During the overexpression of protein, if the animal cell transformed with expression vectors of the present invention is used, amounts of ammonia accumulation within cell culture medium will decrease and cell growth rate will increase, thus, it is advantageous to be capable of obtaining desired protein in high yields.

The present invention relates to expression vectors containing in vivo urea cycle enzyme gene; transformants thereof; and the use of transformants for protein overexpression. During the overexpression of protein, if the animal cell transformed with expression vectors of the present invention is used, amounts of ammonia accumulation within cell culture medium will decrease and cell growth rate will increase, thus, it is advantageous to be capable of obtaining desired protein in high yields.

The invention discloses a cell line overexpressing CIAPIN1 protein and its preparation method and application; the cell line name is BHK-21 CIAPIN1+, the preservation number is CCTCC C2014149, and the amount of the expressed CIAPIN1 protein is 10 times that of normal BHK-21 cells. times; the preparation method of this cell line is to insert the cDNA sequence of CIAPIN1 into the eukaryotic expression plasmid pIRESneo, and then transfect BHK‑21 cells; after being screened by G418-containing culture medium, the cells are obtained by limiting dilution method to obtain positive monoclonal cells, namely Cell lines overexpressing CIAPIN1 protein were immortalized. The cultivation of the rabies virus by the cell line of the present invention can significantly affect the titer of the virus, and is of great significance to the efficient cultivation of the virus and the production of the inactivated vaccine. The cell line of the present invention has a good application prospect in the cultivation of the rabies virus.

The invention provides an XNDL2 gene, a protein, an overexpression vector, an acquisition method for rice resistant to rice sheath blight disease and application of the XNDL2 gene, belonging to the technical field of biology. The nucleotide sequence of the XNDL2 gene is shown as SEQ ID No. 1. The XNDL2 gene provided by the invention has the effect of resisting rice sheath blight disease. When theXNDL2 gene is introduced into rice, the rice capable of resisting rice sheath blight disease can be obtained.

The present invention obtains, by PET, images of neurogenic inflammation, cerebral apoplexy and tumors, which are associated with new translocator protein overexpression, through a fluorine-18-labeledtranslocator protein overexpression-targeting PET radiotracer, thereby diagnosing patients with various brain diseases and tumors, and providing a brain neurogenic inflammation and tumor imaging diagnosis to more patients in comparison to a conventional carbon-11-tracer because of the long half-life of fluorine-18.