205 results about "Peptide library" patented technology

Peptide sequences capable of binding to insulin and/or insulin-like growth factor receptors with either agonist or antagonist activity and identified from various peptide libraries are disclosed. This invention also identifies at least two different binding sites, which are present on insulin and insulin-like growth factor receptors, and which selectively bind the peptides of this invention. As agonists, certain of the peptides of this invention may be useful for development as therapeutics to supplement or replace endogenous peptide hormones. The antagonists may also be developed as therapeutics.

A minicell display method has been developed which has significant advantages for screening peptide libraries for candidates that can bind and effectively modulate a particular biological process. The method, based on the small, anucleate minicell, has increased versatility in generating unique sequences to screen as well as increasing the size of the peptides to be screened. In vivo mutagenesis, at the level of protein synthesis, as well as DNA replication, increases diversification of the library to be screened and therefore substantially increases the number of potential peptides that can modulate a particular biological response or mechanism.

The present invention provides a method of determining or identifying or isolating a cell-penetrating peptide (CPP) or analog or derivative thereof having cell-type selectivity and/or at least capable of passing through a Blood Brain Barrier of an animal subject. This invention also provides CPPs and analogs and derivatives thereof, such as those set forth in SEQ ID NOs: 1-27 of the Sequence Listing, and compositions comprising one or more of the CPPs, including conjugates in which a CPP or analog or derivative thereof is linked to a cargo molecule. The invention also provides methods for transporting cargo molecules across cell membranes to specific locations within cells, and for treating, preventing and/or diagnosing diseases that are treatable by a cargo molecule to which a CPP or analog or derivative of the invention is attached. The invention also provides tailored peptide libraries for use in identifying or isolating CPPs.

The present invention encompasses a method for expanding an antigen specific T cell from a population of cells. The method of the present invention comprises contacting a population of cells with an MHC restricted antigenic glypican-3 peptide. In some instances, the T cell is expanded using monocyte-derived dendritic cells and defined MHC restricted antigenic glypican-3 peptides. In one embodiment, high-affinity antigen-specific T-cell receptors (TCRs) can be cloned from the antigen specific T cell.

The present invention relates to a versatile method of identifying protein coding DNA sequences (genes) useful as drug targets in a genome using specially developed software GeneDecipher, said method comprising steps of generating peptide libraries from the known genomes with peptide of length ‘N’ computationally arranged in an alphabetical order, artificially translating the test genome to obtain a polypeptide corresponding to each reading frame, converting each polypeptide sequence into an alphanumeric sequence one corresponding to each reading frame on the basis of overlappings with the peptide libraries, training Artificial Neural Network (ANN) with sigmoidal learning function to the alphanumeric sequence, deciphering the protein coding regions in the test genome, thus, identifying longer streches of peptides mapping to large number of known genes and their corresponding proteins and lastly, a method of the management of the diseases caused by the pathogenic organisms comprising a step of evaluation of the proposed drug candidate by inhibiting the functioning of one or more proteins identified by the steps of the invention.

Peptide libraries containing peptides having the same net charge or same like charge are described, which may be used to screen for substrates of kinases and phosphatases and any other enzyme that causes a difference in net charge in a suitable substrate. The libraries are designed using representative amino acids for one or more classes of amino acids, thereby reducing the number of members in the peptide library. Reducing the number of peptides in the library to a manageable size permits the peptides to be segregated into individual wells of a microtiter plate, where the sequences of suitable substrates are immediately ascertainable upon exposure to enzyme by detecting the position of charge inverted peptide substrates in the plate.

The present invention discloses a kind of small peptide with the amino acid sequence of P-M-K-M-R-T-M or Y-E-P-K-I-R-G, and its preparation. The preparation process includes screening bacteriophage peptide library with B lymphocyte stimulus factor as target molecule to obtain B lymphocyte stimulus factor specifically combined small peptide and determining its sequence, and subsequent artificial synthesis of corresponding small peptide. The small peptide may be used in preparing medicine for treating B lymphocyte stimulus factor related diseases.

The invention discloses a tumor neoantigen screening method fused with single cell TCR sequencing data. The tumor neoantigen screening method comprises the following steps: based on whole exon sequencing data and transcriptome sequencing data, carrying out quality control, comparison and other steps through software to obtain a neomutant peptide library; predicting an HLA-I type typing by using HLA typing prediction software; in combination with single-cell TCR sequencing and single-cell transcriptome sequencing, finding cancer-specific CD8 + T cell receptors through cell type annotation and clone frequency analysis; meanwhile, based on integrated deep learning, the short peptide immunogenicity is identified through a peptide-TCR interaction prediction model, a tumor neoantigen screening method fused with single cell TCR sequencing data is provided, and the problems that a traditional tumor antigen screening method is high in neoantigen misselection and missed selection rate, insufficient in immunogenicity and the like are solved.

The invention relates to a polypeptide with tumor-targeting performance and a preparation method. The structure of the aminophenol sequence of the polypeptide is CASPSGALRSC or CFPVPGHDLVC or CFSVPGHDIVC or CTPMSLSLSEC or CYTYPLGWHIC. The pC89 phage peptide library expressing protein polypeptides with different sequences of 10⁸ and human breast cancer cell lines MDA-MB-231 are repetitively cocultured for a few times; filtration can penetrate cell membrane to enter into the phages expressing specific polypeptide in cytolymph and / or karyon, and phages are amplified in vitro in order to carry out DNA sequencing and deduce an exogenous amino acid sequence inserted in the phages; the filtered specific polypeptide phages, other human tumor cells and normal cells are cocultured in vitro, and the tumor cell specificity of the polypeptide phages is tested; according to the testing results of polypeptide sequence and cell specificity, the polypeptide with tumor targeting is artifically synthesized. The invention as a specificity carrier of the mammary cancer-targeting genetic therapy has potential clinic application value. The invention also provides a strong technical support for the filtration of affinity specificity polypeptide of other types of malignant tumor cell strains. Moreover, the polypeptide only contains nine to eleven amino acid residues, so the polypeptide can be easily synthesized, the change of spacial position is relatively less, the quality control of the polypeptide is easy, and use is convenient.

A method for determining protein binding specificity using a screen of a peptide library is provided. The method can be used to determine binding specificity for human NAD⁺-dependent deacetylase SIRT1, and to identify the most efficiently deacetylated peptide sequences. The method can be also used to screen a combinatorial H4 histone N-terminal tail peptide library to examine the binding preferences of a alpha-phos (S1) H4 antibody toward all known possible H4 histone modification states.

Screening libraries of peptides in different assays offers an opportunity to simultaneously interrogate intracellular signaling pathways, create reagents to further the understanding of the pathway, and to create novel forms of therapies.

Many, if not all, biologically active peptides (e.g. peptide hormones) have profound effects both in health and disease, either by growth stimulating roles, growth inhibitory roles, or the regulation of critical metabolic pathways.

The present invention is directed to novel bioactive peptides, an in silico method to identify these peptides and a peptide library containing these peptides.