The invention discloses an automatic focusing method and an
image acquisition device, wherein the device comprises at least one
imaging lens, at least one focusing lens, a photosensitive device and a
focal length regulation mechanism. The method comprises the following steps that: A. according to the actual scale of an object, the actual scale of an image formed on the photosensitive device by the object and the distance between the photosensitive device and the
imaging lens, the product of the distance between the photosensitive device and the
imaging lens and the actual object scale divided by the sum of the actual object scale and the actual
image scale equals a focusing
focal length estimation value FF; B. the
focal length regulation mechanism is regulated to allow the focal length to reach the focusing focal length
estimation value FF; C. the focal length is fine-adjusted to a plurality of focal length positions around the focusing focal length
estimation value FF; the focusing estimation value of the image formed on each focal length position is calculated, and the focal length corresponding to the maximum focusing estimation value is taken as an accurate focusing focal length MF; D. the focal length regulation mechanism is regulated to allow the focal length to reach the accurate focusing focal length MF, and then the automatic focusing process is accomplished.