The invention discloses an intelligent biomass fuel acousto-optic recognition sampling system and a method thereof. All inside and outside properties of biomass fuel are detected, judged and displayed in real time through cooperation of a detection control unit, a data acquisition unit, a fuel expert analysis unit and a quality control unit. The technological means is advanced, has high accuracy, stable detection result and high efficiency, has sampling automation without man-made influence and artificial drag, has positive meaning on promotion of production efficiency and management methods, can measure inside and outside moisture of the biomass fuel in real time, can observe mineral materials in the biomass fuel by graphs, intelligently judge and measure the material volume, can analyze fuel quality according to real-time detection data and make a judgment, and can effectively promote the progress and development of production and technology.