Method for detecting dihydroxybenzene in water body by colorimetric method

A technology of dihydroxybenzene and colorimetry, applied in the field of chemical analysis, can solve the problems of high operation requirements, complicated preparation steps of modified electrodes, and high cost

Pending Publication Date: 2022-03-08
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the preparation steps of the modified electrode are complicated, the operation requirements are high, and it is difficult to be widely used in practice due to the problems of long detection time, complicated operating conditions, high cost, and poor specificity.

Method used

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  • Method for detecting dihydroxybenzene in water body by colorimetric method
  • Method for detecting dihydroxybenzene in water body by colorimetric method
  • Method for detecting dihydroxybenzene in water body by colorimetric method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] (1) Weigh 0.1g of "Cicada Pupa" structure (such as figure 1 Shown) CeO 2 (pore diameter 5nm, porosity 60%) and 0.0558gMnSO 4 4H 2 O, dissolve in 50mL water, ultrasonically disperse evenly, and stir with a magnetic stirrer for 60min, so that manganese ions can be evenly loaded on the white CeO 2 pores and surfaces.

[0057] (2) In order to increase the interaction between the above materials and the chromogenic molecule (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, ABTS), and to slow down the Mn element in the actual test process For the loss in the medium, add 0.05mL 0.01mM cyclodextrin (Cyclodextrin, referred to as CD) to the system obtained in step (1), react for two hours, centrifuge with a high-speed centrifuge, take out the supernatant, wash at least three times, and finally The washed material was vacuum dried at 60°C.

[0058] (3) Disperse 20 mg of the material in step (2) in 4 mL of acetone, use a spray gun to 2 Spray the suspension onto a piece ...

Embodiment 2

[0065] (1) take by weighing the CeO of " cicada chrysalis " structure in 0.1g embodiment 1 2 and 0.0558gMnSO 4 4H 2 O, dissolved in 50mL of water, ultrasonically dispersed, stirred with a magnetic stirrer for 60min, so that manganese ions can be evenly loaded on the white CeO 2 pores and surfaces. Centrifuge with a high-speed centrifuge, take out the supernatant, wash at least three times, and finally vacuum-dry the washed material at 60°C.

[0066] (2) Disperse 20mg of nanozymes in 4mL of acetone, use a spray gun to 2 Spray the suspension onto a piece of test paper placed vertically, and dry it at room temperature for later use.

[0067] (3) Take 120 μL of 1mM TMB and 10 μL of hydroquinone in different concentrations (resorcinol, catechol and hydroquinone respectively), stir for 1 min, take 200uL of the mixed liquid and drop it on the test paper prepared in step 2 , the excess water permeates the test paper, and the TMB molecules remain on the test paper and are further ...

Embodiment 3

[0073] (1) Weigh 0.1g of CeO with "cubic" structure 2 (Such as figure 2 Shown, pore diameter 55nm, porosity 7%) and 0.0558gMnSO 4 4H 2 O, dissolved in 50mL of water, ultrasonically dispersed, stirred with a magnetic stirrer for 60min, so that manganese ions can be evenly loaded on CeO 2 On the surface.

[0074] (2) In order to increase the interaction between the above materials and the chromogenic molecule (3,3',5,5'-tetramethylbenzidine, TMB), and slow down the loss of Mn element during the actual test, in step (1 ) on the basis of adding 0.05mL 0.01mM chitosan (chitosan), after two hours of reaction, centrifuge with a high-speed centrifuge, take out the supernatant, wash at least three times, and finally vacuum-dry the washed material at 60°C.

[0075] (3) Disperse 20mg nanozymes in 4mL ethanol, use a spray gun to 2 Spray the suspension onto a piece of test paper placed vertically, and dry it at room temperature for later use.

[0076] (4) Take 120 μL of 1mM ABTS, 10...

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Abstract

The invention provides a method for detecting dihydroxybenzene in a water body by a colorimetric method. The method comprises the following steps: A) preparing cerium oxide loaded with Mn < 2 + >; furthermore, an accelerant can be added to carry out coordination encapsulation on the Mn element, so that the hydrophilicity of the particles is improved, and the adsorption effect of the particles on signal molecules is enhanced; b) dispersing the cerium oxide loaded with Mn < 2 + > in an organic solvent, coating the surface of the test paper with the cerium oxide, and drying to obtain the test paper; c) respectively mixing chromogenic substrate molecules with dihydroxybenzene standard solutions with different concentrations, dropwise adding the mixture to the surface of the test paper, and establishing a standard curve of color chromatic values and concentrations; and D) mixing the chromogenic substrate molecules with a water body sample to be detected, dropwise adding the mixture to the surface of the detection test paper, and obtaining the concentration of the dihydroxybenzene according to the chromatic value of the color. The detection method disclosed by the invention has the advantages of high stability, high efficiency, low cost, strong specificity and the like, is mild in detection condition, saves time and economic cost, and has an extremely good industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis, in particular to a colorimetric method for detecting dihydroxybenzene in water. Background technique [0002] Hydroquinone, catechol, and resorcinol are isomers of dihydroxybenzene, which belong to phenolic substances. They are important intermediates in the production of monoalkyl ethers and dialkyl ethers. They are used as Photographic developer, paint stabilizer and excellent decolorizer. However, phenols have the characteristics of high toxicity and low degradation. When they enter the ecosystem, they will affect the health of humans, animals and plants, and are the main pollutants in the water environment. Therefore, it is particularly important for analytical chemistry and organic chemistry to develop a stable, high-sensitivity, and ultra-convenient method for the analysis of dihydroxybenzene. [0003] At present, there are high performance liquid chromatography, chemiluminescen...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 吴生焘林英武詹璇黄刚强
Owner NANHUA UNIV
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