Method for detecting common plant growth regulator in culture medium

Abstract

The invention provides a method for detecting a common plant growth regulator in a culture medium. The method comprises the following steps: fully mashing and uniformly mixing a sample according to the following substance proportion: 10g of the sample is put into a 50ml centrifuge tube; transferring into 10ml of acetonitrile, and oscillating for 20 minutes at the speed of 2500 rpm; adding 1.14 g of sodium chloride and 2.24 g of anhydrous sodium sulfate into each tube, and evenly mixing the mixture for multiple times in an upside-down mode; continuously oscillating for 20 minutes at the speed of 2500 rpm; centrifuging for 5 minutes at the speed of 5000 rpm at the temperature of 20 DEG C so as to realize layering; absorbing a proper amount of acetonitrile layer, and filtering with a 0.22 mu m filter membrane to obtain computer liquid; preparing a standard solution; carrying out liquid chromatography analysis; drawing a standard curve, and calculating the concentration of each component in the computer liquid of each sample according to the peak area. According to the method, a QuEChERS method commonly used in pesticide residue detection is used as reference, and the variety and dosage of salt are optimized. As the matrix components of the sample are relatively simple, the purification step is omitted, the detection time is shortened, and the precision is higher.

Description

technical field [0001] The invention belongs to the technical field of agricultural residue detection, and in particular relates to a method for detecting common plant growth regulators in culture medium. Background technique [0002] Due to the needs of the experiment, the concentration of various plant growth regulators in the medium needs to be measured. [0003] After consulting the relevant literature, there is no similar method. In the literature that has been consulted, such as "HPLC method and HPLC-MS / MS method for the detection of plant growth promoter residues in fruits and vegetables" (Zhang Ying, 2012) is for fresh fruit and vegetable samples, and the sample processing steps are "extraction→evaporation of solvent→ Recovery to constant volume", the advantage of this treatment method is that it can eliminate the influence of the volume of the sample itself on the final constant volume, but the operation is more cumbersome; such as "solid phase dispersion extractio...

AI Technical Summary

Problems solved by technology

In the literature that has been consulted, such as "HPLC method and HPLC-MS / MS method for the detection of plant growth promoter residues in fruits and vegetables" (Zhang Ying, 2012) is for fresh fruit and vegetable samples, and the sample processing steps are "extraction→evaporation of solvent→ Recovery to constant volume", the advantage of this treatment method is that it can eliminate the influence of the volume of the sample itself on the final constant volume, but the operation is more cumbersome; such as "solid phase dispersion extraction-high performance liquid chromatography for the determination of plant growth regulators in tomato paste "(Zhou Jianke, 2011) is aimed at pasty samples, and the sample processing steps are relatively cumbersome, and there is room for optimization in the detection wavelength and mobile phase selection, "High Performance Liquid Chromatography for Naphthaleneacetic Acid Residues" (Kong Deyang, 2010) , "HPLC Determination of α-Naphthylacetic Acid in Taxus Cell Culture" (Huang Qiaoming, 2012), "Residue Detection of α-Naphthylacetic Acid in Tomato and Rice" (Guo Suyi, 2008), etc. There are also similar problems; another example is "GB / T 37500-2019 Determination of Plant Growth Regulators in Fertilizers by High Performance Liquid Chromatography", "DB37 / T 3760-2019 Fertilizers of Abscisic Acid, Naphthaleneacetic Acid, Forchlorfenuron, Enzyme Determination of Azole by High Performance Liquid Chromatography", although the sample processing steps are simple, but the sample matrix is ​​completely different from this test, a small amount of fertilizer dissolved in the solvent has almost no effect on the final volume, while directly adding the solvent to the medium sample must consider the volume Therefore, it cannot meet the needs; another example is "Determination of Sucralose in Jelly by High Performance Liquid Chromatography" (Wang Zhongyi, 2017), "Simultaneous Determination of 11 Synthetic Sucralose in Jelly, Chewing Gum and Gelatin Capsule Shell by High Performance Liquid Chromatography" Colorants and their 8 kinds of aluminum lakes" (Zhao Mei, 2019), "Simultaneous Determination of 9 Additives in Jelly by High Performance Liquid Chromatography" (Qian Cong, 2014), the sample matrix is ​​similar to this test, all of which are gels sample, the sample processing method is heating to dissolve the gel, supplemented by shaking and ultrasonic to dissolve the substance to be tested, but in this test, the thermal stability of the substance to be tested has yet to be verified, and this method is not applicable for the time being

Images