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Biological sample safety protective agent and application thereof

A technology for biological samples and safety protection, applied in the fields of biology and medicine, can solve problems such as chemical pollution, protein disintegration and inactivation, and huge impact on test results, and achieve the effects of fast reproduction, low nutritional requirements, and enhanced bactericidal effect

Inactive Publication Date: 2021-01-08
珠海市德灏生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, all inspection items are carried out before the biosafety risk of the sample is eliminated. The operator and the experimental environment are at biosafety risk throughout the inspection process. Results of Biosample Safeguards
[0006] Using traditional disinfection preparations and methods to kill pathogens can eliminate the biosafety risk of samples, but it has a huge impact on subsequent test results and has no practical significance: 1) Use chemical disinfectants to kill: powerful disinfectants include aldehydes and Strong oxidants and aldehydes are easy to produce pungent odors and cause new chemical pollution; strong oxidants have obvious interference on dyeing, especially fluorescent dyeing, often leading to fluorescence quenching
Medium and low-efficiency disinfectants such as quaternary ammonium salts, surfactants, etc. are slow to act and cannot eliminate the biosafety risk of samples in a short time
2) Sterilization by heat sterilization: it will lead to the denaturation and cracking of the formed components of the sample, and the disintegration and inactivation of proteins, enzymes, antigens, antibodies, nucleic acids, etc.
3) The remaining samples and cultures are temporarily stored for centralized processing: the risk is difficult to control during the temporary storage period, and the existing measures are all passive measures
[0007] The current biosafety protection measures require a lot of resource investment: biosafety laboratories need to be established to ensure environmental safety; personal protective equipment needs to be equipped to ensure personnel safety; all operations are required to be operated in biosafety cabinets as much as possible to ensure the safety of the inspection process; The above requirements may even lead to changes in the functional layout of the laboratory

Method used

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  • Biological sample safety protective agent and application thereof
  • Biological sample safety protective agent and application thereof
  • Biological sample safety protective agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The specific steps of the experimental research method of this application are:

[0051] 1. Bactericidal test method

[0052] 1. Preparation of test bacterial solution: select Staphylococcus aureus, Bacillus subtilis, Legionella pneumophila, and Candida albicans solid plate cultures in the logarithmic growth phase, and scrape the colonies into sterile saline respectively. Adjust the bacterial concentration to 0.5 McFarland turbidity, and then make a 1:1000 dilution to make the bacterial concentration about 5*10 5 CFU / ml, made into 4 kinds of test bacterial solutions;

[0053] 2. Divide each bacterial solution into two parts, one part is used for bactericidal test, and the other part is used for control test;

[0054] 3. Sterilization: Add an equal volume of the bactericide to be tested to the test tube / well, and add an equal volume of sterile saline to the control tube / well;

[0055] 4. Inoculation: 10min, 20min, 30min, and 45min after mixing, pipette the liquid in t...

Embodiment 2

[0074] Embodiment 2 minimum inhibitory concentration test

[0075] 1. Minimum inhibitory concentration test of hydrogen peroxide system

[0076] Hydrogen peroxide is produced by oxidase-catalyzed decomposition of substrate. When the amount of substrate is large enough, the production of hydrogen peroxide is controlled by the amount of enzyme.

[0077] Preparation of different concentrations of hydrogen peroxide sterilizing solution: the mass percent concentration of the prepared glucose aqueous solution is 5%, the concentration of the glucose oxidase stock solution is 12800u / ml, after the glucose oxidase contacts with the glucose, hydrogen peroxide can be continuously produced to form a sterilizing solution. The sterilizing solutions with different concentrations were prepared by doubling dilution method in a sterile plastic microwell plate. Wells 1-8 contained hydrogen peroxide, and well 9 was a control without hydrogen peroxide, as shown in Table 2.

[0078] Table 2

[007...

Embodiment 3

[0108] One, the preparation of the safety protective agent of the present invention, concrete proportioning is as shown in table 11:

[0109] Table 11

[0110]

[0111] Bisdecyldimethylammonium chloride solution, MIT solution, and CMIT solution were all prepared with deionized water.

[0112] Prepare component A and component B according to the above ratio, and then use 0.22um microporous membrane to filter and sterilize, and set aside. Component A and component B are added into the biological sample to be tested in equal volumes during use, and the sum of the volumes of component A and component B is the same as the volume of the biological sample to be tested.

[0113] 2. The disinfecting effect of safety protective agent on pathogenic bacteria

[0114] (1) Carry out with reference to the aforementioned "one. Bactericidal test method", get the bacterium liquid of each kind of test bacteria, and the amount of substances added to the test tube and the control tube is as s...

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PUM

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Abstract

The invention discloses a biological sample safety protective agent which comprises a component A and a component B. The component A comprises oxidase and an enzyme stabilizer. The component B comprises a substrate corresponding to oxidase in the component A, long-chain quaternary ammonium salt and a specific enzyme inhibitor, wherein hydrogen peroxide can be generated after the oxidase meets thesubstrate corresponding to the oxidase; and the specific enzyme inhibitor can specifically inhibit tricarboxylic acid circulation and electron transfer chain associated enzymes, and does not interferewith the activity of key enzymes in a diagnostic reagent for biological sample detection. The invention also discloses an application of the biological sample safety protective agent in sterilizationand disinfection treatment of a pathogen-containing biological sample, and a method for sterilization and disinfection treatment of the biological sample by using the safety protective agent. The biological sample safety protection agent can eliminate the safety risk of the biological sample, does not influence the subsequent inspection result, is environment-friendly, and does not increase the hardware investment or increase the personnel burden.

Description

technical field [0001] The invention relates to the technical fields of biology and medicine, in particular to a biological sample safety protectant and its application. Background technique [0002] In medicine, clinical samples have great biosafety risks. For example: respiratory samples (such as sputum, throat swabs, tracheal lavage fluid, etc.) can carry Mycobacterium tuberculosis, influenza virus, SARS virus, new coronavirus, rubella virus, Mumps virus, respiratory syncytial virus, mycoplasma pneumoniae, meningococcus, etc.; blood samples can carry HIV, hepatitis B virus, hepatitis C virus, Salmonella typhi, etc.; genitourinary tract samples can carry gonorrhea, Treponema pallidum, Ureaplasma urealyticum , Chlamydia trachomatis, papillomavirus, herpes simplex virus, etc.; stool samples can carry Salmonella typhi, Shigella dysenteriae, Campylobacter jejuni, rotavirus, enterovirus, etc.; skin lesion tissue samples often carry yeast-like fungi, filamentous Fungi, gastric ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/50A01N59/00A01N33/12A01N43/80A01P1/00A01P3/00
CPCA01N33/12A01N43/80A01N59/00A01N63/50
Inventor 农高惠何林声
Owner 珠海市德灏生物科技有限公司
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