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A kind of in vitro culture medium of immature oocyte and its preparation method and application

An in vitro culture and oocyte technology, applied in the field of assisted reproduction, to achieve the effect of promoting the maturation of cytoplasm, single composition, and recovery of meiosis

Active Publication Date: 2022-05-06
成都艾伟孚生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a technical solution that can make the nucleus and cytoplasm of immature oocytes mature synchronously, and improve the fertilization rate and high-quality embryo rate has not yet been proposed.

Method used

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  • A kind of in vitro culture medium of immature oocyte and its preparation method and application
  • A kind of in vitro culture medium of immature oocyte and its preparation method and application
  • A kind of in vitro culture medium of immature oocyte and its preparation method and application

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preparation example Construction

[0056] 2. The preparation method of in vitro culture fluid in embodiment 1~5, comprises the following steps:

[0057] S1. prepare solution I, comprise the following steps:

[0058] 1) Make the required utensils for preparation go through dry heat at 250°C for 30 minutes to remove the heat source;

[0059] 2) Under 100-grade conditions, weigh calcium chloride, potassium chloride, magnesium sulfate, sodium chloride, sodium bicarbonate, sodium dihydrogen phosphate hydrate, arginine, cystine, cysteine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Tyrosine, Valine, Asparagine, Aspartame amino acid, glutamic acid, N(2)-L-alanyl-L-glutamine, glycine, proline, serine, calcium D-pantothenate (B5), choline chloride, folic acid (B9), Inositol (B8), Niacinamide (B3), Pyridoxal Hydrochloride (B6), Riboflavin (B2), Thiamine (B1), Ascorbic Acid (C), D-Glucose, Sodium Pyruvate, Sodium Lactate and Chin Damycin, and add water and mix uniformly to ...

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Abstract

The invention relates to the field of assisted reproductive technology, and discloses an in vitro culture solution of immature oocytes, including a basic culture solution and a composition, the basic culture solution includes a solvent and a solute, the solvent includes water, and the solute includes Vitamins, energy substances, inorganic salts and amino acids; the composition includes butyrolactone-I 0.01-1mg / L, estradiol 0.001-2mg / L, follicle-stimulating hormone 0.001-1IU / L, luteinizing hormone 0.001-1IU / L, epidermal growth factor 0.001~1IU / L, insulin-like growth factor 0.001~1mg / L and human chorionic gonadotropin 0.001~1IU / L; the invention also discloses the preparation method and application of the in vitro culture solution; In addition to the addition of basic substances, the invention achieves synchronous maturation of the nucleus and cytoplasm of immature oocytes and increases the fertilization rate and high-quality embryo rate by adding the energy substance, composition and vitamins.

Description

technical field [0001] The invention relates to the technical field of assisted reproduction, in particular to an in vitro culture solution of immature oocytes and a preparation method and application thereof. Background technique [0002] In vitro fertilization (in vitro fertilization, IVF), also known as test-tube baby, is a method of assisted reproductive therapy. Specifically, it refers to taking out eggs and sperm, performing in vitro fertilization, culturing and forming embryos, and then transferring the embryos back to the mother's uterus The inner pregnancy develops into a fetus. [0003] Assisted reproductive technology (ART) has come a long way since the first test-tube baby was born in 1978. At first, assisted reproductive technology was to obtain mature eggs in a woman's normal menstrual cycle for in vitro fertilization, but a woman only matured and eliminated one egg in one ovulation cycle. Because of the small number, the success rate of assisted reproductive ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2500/90C12N2501/11C12N2501/31C12N2500/30C12N2500/38
Inventor 余裕炉张瑜米乐贾晓伟刘洪君严飞戴甄
Owner 成都艾伟孚生物科技有限公司
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