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Method for biosynthesis of organic selenium by using Hypsizygus marmoreus

A seafood mushroom and organic selenium technology, applied in the field of microbiology, can solve the problems of no MeSeCys and other problems, achieve the effect of controllable selenium addition, controllable cultivation conditions, and increase yield

Active Publication Date: 2020-06-12
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently reported MeSeCys are mainly through chemical synthesis, see CN200710051362.3 and CN200910115585.0, etc., but no biosynthetic method is used to obtain MeSeCys

Method used

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  • Method for biosynthesis of organic selenium by using Hypsizygus marmoreus
  • Method for biosynthesis of organic selenium by using Hypsizygus marmoreus

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 strain breeding and the acquisition of selenium-enriched carrier

[0033] The seafood mushroom strain H023 preserved in the Edible Mushroom Research Office of China Agricultural University was cultured on PDA medium for 14 days, and 5 mm of bacterial cakes were taken from the growth edge of the colony to inoculate sodium selenate and sodium selenite at 1.0 mg / kg respectively. , 5.0mg / kg and 20.0mg / kg plates, cultivated at 21°C for 10 days, and measured the colony diameter. The results showed that H023 colony growth was significantly inhibited under the treatment of 5.0mg / kg sodium selenite; at 25.0mg / kg Sodium selenate treatment significantly inhibited the growth of H023 mycelia ( figure 2 ).

[0034] Cultivate the seafood mushroom strain H023 preserved in the Edible Mushroom Research Office of China Agricultural University on PDA medium for 14 days, take 5mm fungus cakes from the growth edge of the colony and inoculate them on 10 PDA plates and culture t...

Embodiment 2

[0036] Embodiment 2 sodium selenate treatment selenium-enriched cultivation

[0037] The weight ratio of Douglas fir sawdust, corn cobs, soybean hulls, and wheat bran with uniform particle size is 40:30:15:15, the pH is adjusted to 8 with lime powder, the moisture content is 80%, and the selenate concentration gradient is set to 0.5, 1.0, 2.5, 5.0, 10.0, 20.0, 40.0mg / kg, each culture bag is filled with 2.0kg of mixed material, 121 ℃ high temperature, autoclave for 2 hours. After cooling to room temperature, the bacteria were inoculated. Take 5mm uniform bacterium cake from the test tube for preserving the strain and inoculate it into a 500mL Erlenmeyer shaker flask filled with 150mL liquid PDA medium, and shake it at 170-180r / min at 21-23°C for 4 days to become the first-grade bacteria kind. Use a sterile pipette to draw 5mL of bacteria from the first-level strain shake flask covered with mycelium balls and transfer it to a 500mL shake flask containing 150mL of PDA liquid me...

Embodiment 3

[0038] Embodiment 3 Sodium Selenite Treatment Selenium-enriched Cultivation

[0039] Use Douglas fir sawdust, corn cobs, soybean hulls, and wheat bran with a weight ratio of 40:30:15:15, use lime powder to adjust the pH to 8, the moisture content is 80%, and the selenite concentration gradient is set 0.5, 1.0, 2.5, 5.0, 10.0, 20.0, 40.0 mg / kg, each culture bag is loaded with 2.0 kg of mixed material, and sterilized in a high temperature and high pressure autoclave at 121 °C for 2 hours. After cooling to room temperature, the bacteria were inoculated. Take a 5mm uniform bacterium cake from the test tube for preserving the strain and inoculate it into a 500mL Erlenmeyer shaker flask filled with 150mL liquid PDA medium, and culture it with shaking at 170-180r / min at 21-23°C for 4 days as the first-grade bacteria kind. Use a sterile pipette to draw 5mL of bacteria from the first-level strain shake flask covered with mycelium balls and transfer it to a 500mL shake flask containin...

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Abstract

The invention provides a method for biosynthesis of organic selenium by using Hypsizygus marmoreus. The invention provides a Hypsizygus marmoreus mutant strain which can be obtained through ultraviolet mutagenesis, inorganic selenium in a culture medium can be converted efficiently into organic selenium through the Hypsizygus marmoreus mutant strain, and the strain has a preservation number of CCTCC M 2019981, wherein Hypsizygus marmoreus has short fruiting time and short growth time, controllable culture conditions, controllable addition amount of selenium and a stable growth state. Sodium selenate, sodium selenite and nano selenium are preferably adopted as the addition forms, no effects on the biomass of fruiting bodies of Hypsizygus marmoreus are caused under the condition of a concentration of 40 mg / kg, but increase of the yield of the fruiting bodies of Hypsizygus marmoreus can be promoted to a certain extent, wherein selenium-methyl selenocysteine can reach up to 39.4% of the total content of selenium. During culture, mycelia of Hypsizygus marmoreus grow fast, fungus bags can be full in a short time, and Hypsizygus marmoreus has white and tender color and high yield, so thata powerful means is provided for preparation of selenium-methyl selenocysteine and other organic selenium.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to a method for biosynthesizing organic selenium by using seafood mushrooms. Background technique [0002] Seafood mushroom belongs to Basidiomycotina, Laminomycetes, Agaricales, White Mushrooms, and Amygium genus. It is a large woody saprophytic fungus with delicious meat and excellent taste. Seafood mushrooms are rich in amino acids, among which the content of lysine and arginine is higher than that of common mushrooms, which helps teenagers improve their intelligence. Seafood mushroom extract has a variety of physiologically active ingredients, among which fungal polysaccharides, purine, and adenosine can enhance immunity, promote antibody formation, prevent aging, fight cancer, and delay aging. At the same time, seafood mushroom is also a low-calorie, low-fat health food. [0003] Selenium is an essential trace element for the human body. The human body cannot synthesize selenium s...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20A23L31/00A61K36/07A61K8/9728A61Q19/00
CPCA61K36/07A61Q19/00A23L31/00A61K8/9728A01G18/00A01G18/20
Inventor 郭岩彬胡婷赵桂慎丁惠惠改芳
Owner CHINA AGRI UNIV
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