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Lipidosome-based method for modifying PEEK (polyetheretherketone) surface with hexadecadrol/minocycline and application

A technology of dexamethasone and minocycline, which is applied in the direction of liposome delivery, active ingredients of tetracycline, medical preparations containing active ingredients, etc., can solve the problems of small molecules that are not suitable for biological activity, and improve the anti-infective activity , the effect of alleviating foreign body reactions

Inactive Publication Date: 2019-09-27
BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pDA is not suitable for direct immobilization of bioactive small molecules into cells such as Mino (a broad-spectrum tetracycline antibiotic) and Dex (a glucocorticoid with anti-inflammatory and osteogenic effects)

Method used

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  • Lipidosome-based method for modifying PEEK (polyetheretherketone) surface with hexadecadrol/minocycline and application
  • Lipidosome-based method for modifying PEEK (polyetheretherketone) surface with hexadecadrol/minocycline and application
  • Lipidosome-based method for modifying PEEK (polyetheretherketone) surface with hexadecadrol/minocycline and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1 Construction of Dex / Mino liposome modification method and its in vitro evaluation

[0074] 1.1 Dex / Mino liposome preparation

[0075] Prepared by thin film dispersion method; accurately weigh appropriate amount of dipalmitoylphosphatidylcholine (DPPC), cholesterol (Chol), distearoyl Put phosphatidylethanolamine-polyethylene glycol-amino (DSPE-PEG2000-NH2) and Dex in an eggplant-shaped flask, add a mixed solvent of methanol and chloroform (1:1, v / v), shake to dissolve the solute; use rotary evaporation Drying machine (75rpm) in a constant temperature water bath (47°C) reduced pressure and dried thoroughly to remove the organic solvent, and a uniform lipid film was formed on the inner wall of the bottle; take an appropriate amount of hydration solution (a mixture of 120mM anhydrous sodium acetate and anhydrous calcium chloride) solution, pH=7.9) into an eggplant-shaped bottle, and ultrasonically hydrate in a constant temperature water bath (47°C) until the ...

Embodiment 2

[0091] Example 2 Preparation and Characterization of Dexamethasone / Minocycline Liposome Modified Polyetheretherketone

[0092] 2.1 Preparation of Dex / Mino liposome-modified polyether ether ketone

[0093] PEEK surface functional modification

[0094] Pure PEEK rod cut into The pure PEEK discs were polished with different types of silicon carbide sandpaper in turn, placed in acetone, absolute ethanol and deionized water for ultrasonic cleaning for 1 h each, and dried at 50 °C.

[0095] Blank liposomes, Dex / Mino liposomes and fluorescently labeled liposomes were all prepared according to the film hydration method, and the specific method was the same as in Example 1.1.

[0096] The preparation method of the functionalized PEEK surface is the same as in Example 1.2. Briefly, first add dopamine powder into Tris buffer (10mM, pH=8.5) and mix well to obtain a dopamine solution (2mg / mL); then put pure PEEK tablets into the dopamine solution to react for 18h (37°C, 70rpm), and...

Embodiment 3

[0115] Example 3 In vitro verification of dexamethasone / minocycline liposome-modified polyether ether ketone

[0116] 3.1 In vitro cytocompatibility test

[0117] It has been reported in previous literature that cell adhesion is the basis for cell function and tissue integration in vivo, and cell proliferation is closely related to the amount of new bone formation; good cell adhesion and proliferation can promote a large amount of bone formation around materials (such as orthopedic / dental implants). Production of bone tissue. Therefore, a common method for evaluating the cytocompatibility of materials in vitro is to detect the ability of cells to adhere and proliferate on the surface of the material. In this study, we verified the adhesion and proliferation of hBMSCs on the surface of Dex / Mino liposome-modified PEEK, which is the key to judging whether the material can be applied in the biomedical field.

[0118] 3.1.1 Solution preparation

[0119] (1) Calcein-AM and PI...

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Abstract

The invention discloses a lipidosome-based method for modifying a PEEK (polyetheretherketone) surface with hexadecadrol / minocycline and an application of the method. Lipidosome loaded with hexadecadrol and minocycline is prepared with a thin film dispersion method and then modifies the PEEK surface. The method comprises following steps: (1), hexadecadrol and lipids are dissolved in a methanol and chloroform mixture (1:1, v / v); (2), methanol and chloroform are removed by a rotary vacuum evaporator, and then a lipid membrane is hydrated by a hydration solution; lipidosome loaded with hexadecadrol is obtained by water bath ultrasonic treatment, and is extruded by a porous polycarbonate membrane; dialysis is performed overnight; (3), lipidosome is mixed with a minocycline storage solution, the mixture is shaken, and lipidosome loaded with hexadecadrol / minocycline is obtained, and is stored for standby application at 4 DEG C after being dialyzed overnight; (4), PEEK implant is put in a dopamine solution for reaction, and ultrasonic cleaning is performed; (5), the PEEK implant is added to a hexadecadrol / minocycline lipidosome solution for soaking and is slightly cleaned, and a product is obtained. The obtained product can promote bone repair, regeneration and integration.

Description

technical field [0001] The invention relates to a liposome-based active small molecule surface modification method and its application, belonging to the technical field of drug controlled release and biomaterials, in particular to liposome-based modification of dexamethasone / minocycline on the surface of PEEK method and application. Background technique [0002] At present, bone defects caused by trauma, bone tumors, infection, inflammation, etc. are still a rapidly growing serious challenge in the medical field worldwide; clinically, orthopedic / dental implants are commonly used hard tissue substitutes for bone defect repair . [0003] PEEK is the material of choice to replace traditional metal implants made of titanium and its alloys. It is a semi-crystalline thermoplastic polymer material with good mechanical properties, chemical stability and thermal stability. Since it was approved by the US Food and Drug Administration in the late 1990s, PEEK has been widely used as a...

Claims

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Application Information

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IPC IPC(8): A61L27/28A61L27/18A61L27/50A61L27/54A61K31/573A61K31/65A61K9/127A61P19/08
CPCA61K9/1271A61K31/573A61K31/65A61L27/18A61L27/28A61L27/50A61L27/54A61L2300/202A61L2300/204A61L2300/406A61L2300/43A61L2300/602A61L2300/606A61L2400/18A61L2420/02A61L2430/02A61P19/08C08L61/16A61K2300/00
Inventor 王立新许晓魏世成
Owner BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
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