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Enzyme quantitative detection method of textile fiber content

A quantitative detection method, textile fiber technology, applied in the direction of weighing by removing certain components, can solve problems such as deviation of results, discharge of toxic and harmful substances, data errors, etc., to achieve elimination of influence, high specificity, and conditions mild effect

Inactive Publication Date: 2019-04-23
余姚市立帅印染有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the physical method, the inspector manually disassembles and weighs the textile or uses a microscope or scanning electron microscope to observe the cross-sectional / longitudinal morphology of the fiber for statistical counting; however, the physical method is a subjective rather than objective test method, and inspectors, especially experienced Insufficient testing personnel have different subjective judgments on fibers, resulting in large differences in test results; at the same time, due to the influence of processing technology, there may be a large difference between the test sample and the standard sample
After the qualitative identification of fiber components by chemical method, different organic reagents / chemical substances (such as hydrochloric acid) and dissolution schemes are used to dissolve different textile fiber components, and the fiber content is determined by the change of textile quality before and after dissolution; but the test process of this method will Toxic and harmful substances are emitted, and the fabric components with similar solubility cannot be distinguished. The solvent dissolution process and scheme are more complicated. The standards of various countries have different regulations on the quality change correction coefficient (d value) when calculating the content, resulting in deviations in the results.
At the same time, some fibers have poor solubility. For some fibers dyed with reactive dyes, chemical reagents cannot completely dissolve them, resulting in data errors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Quantitative detection of fiber content in cotton / polyester fiber / silk / polyacrylonitrile fiber blended fabric dyed with reactive brilliant red X-B

[0028] (1) By infrared absorption spectroscopy and microscope observation, it is determined that the dye type is reactive brilliant red X-B, and the blended fabric fiber component is cotton / polyester fiber / silk / polyacrylonitrile fiber;

[0029] (2) Reactive dye Reactive Brilliant Red X-B can inhibit enzyme activity, so the fabric is pretreated with Yunzhi laccase first, and the reaction conditions are: 2.5% laccase enzyme amount, disodium hydrogen phosphate-potassium dihydrogen phosphate buffer pH5 .8 (ionic strength is 30mM), the mass ratio of textile to buffer is 0.2:1, the reaction temperature is 37°C, and the treatment time is 12h;

[0030] (3) Cut the pretreated fabric into 3 pieces, each of which has a sample weight of 5 grams; then separately treat with acid cellulase, yeast lipase and pepsin. Cellulase reaction con...

Embodiment 2

[0032] Quantitative detection of fiber content in viscose fiber / polylactic acid fiber / wool / polypropylene fiber blended fabric dyed with indigo dye

[0033] (1) By infrared absorption spectrum, microscope observation and melting point method, it is determined that the dye type is indigo dye, and the blended fabric fiber component is viscose fiber / polylactic acid fiber / wool / polypropylene fiber;

[0034](2) Indigo dye is a vat dye, which has little influence on enzyme activity, so no pretreatment is required;

[0035] (3) fabric is cut into the sample that quality is 5g, handles with neutral cellulase, reaction condition is: neutral cellulase enzyme amount 9%, citric acid-sodium citrate buffer pH6.6 (ionic strength 200mM), the mass ratio of textiles and buffer solution is 0.08:1, the reaction temperature is 45°C, and the treatment time is 8h. The residue after degradation is cleaned, dried and weighed (the weight is 3 grams after weighing), according to Change in quality, it can...

Embodiment 3

[0037] Quantitative detection of fiber content in rabbit fur / cupro fiber / polypropylene fiber blended waste textiles dyed with reactive orange X-G

[0038] (1) Through microscope observation and infrared absorption spectrum, it is determined that the dye type is a reactive dye, and waste textiles contain rabbit hair, cupro ammonia fiber and polypropylene fiber;

[0039] (2) Reactive dye Reactive Orange X-G will inhibit enzyme activity, so the fabric is pretreated with peroxidase first, the conditions are: catalase enzyme amount 2.5%, disodium hydrogen phosphate-sodium dihydrogen phosphate buffer pH6 .5 (ionic strength is 80mM), the mass ratio of textile to buffer is 0.2:1, the reaction temperature is 33°C, and the treatment time is 15h;

[0040] (3) Cut the pretreated fabric into 2 pieces, each of which has a sample weight of 5 grams; then use acid cellulase and proteinase K to treat them separately. Acid cellulase reaction conditions are: cellulase enzyme amount 5%, acetic ac...

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Abstract

The invention relates to an enzyme quantitative detection method of the textile fiber content. The enzyme quantitative detection method should have the characteristics of high accuracy, environmentalfriendliness and harmlessness. According to the technical scheme, the enzyme quantitative detection method of the textile fiber content sequentially comprises the following steps: (1) textile fiber components are subjected to qualitative identification; (2) dyed textiles made of a direct dye, an activated dye, an insoluble azo pigment or a cationic dye having a large influence on enzyme activity are adopted and pretreated through oxidordeuctase to remove the dye; and (3) according to a qualitative identification result in the step (1), corresponding cellulase or protease or A enzyme is selected, the multiple textiles treated in the step (2) are selectively and independently degraded one by one, or according to the qualitative identification result in the step (1), the corresponding cellulase or protease or A enzyme is selected to selectively degrade the textiles treated in the step (2) sequentially, wherein the A enzyme is lipase or cutinase.

Description

technical field [0001] The invention belongs to the field of quantitative analysis of textile fiber content, and in particular relates to a method for selective quantitative detection of textile fiber content by using enzymes. Background technique [0002] The composition and content of raw materials used in textiles are not only related to the health of consumers, but also play an important guiding role in the washing and maintenance of products themselves, and are an important basis for consumers to choose products. Therefore, the fiber name and content marked on the fabric label has always been one of the main concerns of consumers, and it is also an important tool for relevant departments to ensure and supervise the quality of textiles. [0003] The national textile industry standard stipulates that the fiber content should be expressed as the percentage of the fiber in the total fiber content of the product. At present, the quantitative detection methods of fiber conten...

Claims

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Application Information

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IPC IPC(8): G01N5/04
CPCG01N5/04
Inventor 周静
Owner 余姚市立帅印染有限公司
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