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A kind of neem leaf extract and its preparation process and application

A technology of extract and neem leaf, which is applied in the field of biotechnology and medicine, can solve the problem of weak antibacterial effect, achieve the effect of inhibiting common bacteria and fungi, good effect, and maintain the normal oral flora

Active Publication Date: 2021-06-18
YUNNAN HIKON BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"In vitro susceptibility of Helicobacter pylori to botanical extracts used traditionally for the treatment of gastrointestinal disorders" published by Mahady et al. pointed out that the MIC of ginkgo biloba leaf methanol extract against Helicobacter pylori was MIC>100μg / mL, and its antibacterial effect was not strong

Method used

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  • A kind of neem leaf extract and its preparation process and application
  • A kind of neem leaf extract and its preparation process and application
  • A kind of neem leaf extract and its preparation process and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: In vitro antibacterial experiment of neem leaf extract

[0027] 1 Antibacterial test in vitro

[0028] 1.1 Experimental bacteria: Helicobacter pylori strain (NCTC11637), Escherichia coli drug sensitivity standard strain (ATCC25922), Staphylococcus aureus drug sensitivity standard strain (ATCC25923), yeast (ATCC90028), mucormycetes (ATCC40241).

[0029] 1.2 Preparation of drug-sensitive paper sheet containing the test solution

[0030] Put a blank drug-sensitive paper sheet with a diameter of 6mm into a petri dish, sterilize it by high-pressure steam at 120°C for 45min, and dry it at 60°C for later use. Use a sterilized pipette tip to draw 20 μL (2.50 g / mL, the concentration is based on the mass of the extract) of the test solution and add it to the sterilized filter paper. A sterilized double-distilled water paper sheet was also prepared as a control.

[0031] 1.3 Preparation of test bacterial solution

[0032] 1.3.1 Helicobacter pylori

[0033] 1.3.1...

Embodiment 2

[0072] Embodiment 2: The extract difference of Neem leaf supercritical extraction and Soxhlet extraction

[0073] 1 The difference in the content of active ingredients

[0074] 1.1 Supercritical extraction of neem leaves

[0075] Neem leaf extract was obtained by extracting Neem leaf according to the optimal process conditions in "4 Process Verification" in Example 1.

[0076] 1.2 Neem leaves were extracted by Soxhlet extraction

[0077] Take 40-50g neem leaf fine powder, extract it with methanol for 10h at 65°C, and spin-evaporate the extract at 50°C to obtain a crude extract. Vacuum-dried with anhydrous copper sulfate to obtain solid or paste Neem Leaf Soxhlet extract.

[0078] 1.3 Content detection experiment of total flavonoids, total terpenoids and total coumarins

[0079] Using Al(NO 3 ) 3 -NaNO 2 -NaOH system colorimetric method to determine the content of total flavonoids in the extract, with rutin as the reference substance. Take 2.5mL of rutin standard soluti...

Embodiment 3

[0090] Example 3: Prescription experiment of neem leaf extract and ginkgo biloba extract in toothpaste

[0091] 1 Ginkgo biloba extract preparation

[0092] Ginkgo biloba extract was prepared according to the method stipulated in "Chinese Pharmacopoeia" (2015 edition) "Ginkgo biloba extract".

[0093] 2 Neem Leaf Extract Preparation

[0094] Carry out the extraction of Neem leaf according to the optimal process condition of "4 process validation" in Example 1 to obtain the crude extract.

[0095] 3 Determination of minimum inhibitory concentration (MIC) of neem leaf extract and ginkgo biloba extract

[0096] Use test tube multiple dilution method, divide into three groups and carry out MIC value determination, the first group measures neem leaf extract, the second group measures ginkgo leaf extract, the third group measures mixed extract (neem leaf extract and ginkgo leaf extract The extracts were mixed with initial concentrations of 25.5 mg / mL and 10.5 mg / mL, respectively)...

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Abstract

The invention discloses a neem leaf extract and its preparation process and application. The present invention obtains neem leaf extract by supercritical carbon dioxide method extraction, and its main medical active ingredient is total flavonoid 20~35%, total terpene 5~10%, total coumarin 2~5%; Helicobacter has obvious inhibitory effect, and it has a synergistic effect when used in combination with Ginkgo biloba extract, so the extract can be used to prepare medicines, health care products, food and daily chemical products that inhibit the activity of Helicobacter pylori, without adding preservatives, bacteriostats, etc. It is especially suitable for the development of toothpaste that inhibits Helicobacter pylori in combination with Ginkgo biloba extract.

Description

Technical field: [0001] The invention relates to the fields of biotechnology and medicine technology, in particular, the invention relates to a neem leaf extract and its preparation process and application. Background technique: [0002] Helicobacter pylori is a Gram-negative bacterium that mainly exists in the mouth and stomach, and is one of the key factors that cause gastric inflammation and gastric cancer. At present, the treatment methods for Helicobacter pylori infection in the stomach are mainly triple or quadruple therapy of 1 to 3 antibiotics. Although this therapy is an oral drug, it cannot deal with the Helicobacter pylori that exists in common oral colonies such as dental plaque, oral mucosa, dental calculus, and gingival pockets. At the same time, the course of antibiotic therapy for Helicobacter pylori is limited, which is prone to bacterial resistance. [0003] Many scholars have shifted their research attention to plant extracts. For example, Han Yan et al....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61K36/58A61P31/04A61P1/02A61P31/10A61K8/9771A61Q11/00A61Q17/00A23L33/105A61K127/00
CPCA23V2002/00A61K36/16A61K36/58A61K2236/37A61K2236/51A61K2800/5922A61Q11/00A61Q17/005A23L33/105A61K8/9771A61K8/9789A61P1/02A61P31/04A61P31/10A61K2300/00A23V2200/308A23V2200/306A23V2250/21
Inventor 陈君吴华铃平夏婷李杰陈岑任红顾煜超乔洪翔尚云龙王建方
Owner YUNNAN HIKON BIOTECH CO LTD
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