A method for extracting limonin in lemon peel residue with guanidinium salt ionic liquid two-phase aqueous phase

A technology of ionic liquid and lemon peel, which is applied in the fields of steroids and organic chemistry, can solve the problems of difficult development and utilization of lemon pomace, slow browning of juice yield, waste of resources, etc., and achieve a simple and feasible extraction method , broad commercial application prospects, the effect of reducing pollution

Active Publication Date: 2020-05-12
重庆汇达生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The taste of lemon fruit is extremely acidic and the proportion of fresh food is low, but it has excellent processing characteristics such as high juice yield and slow browning speed of pulp. Therefore, a large number of fresh lemon fruits are used to extract juice, make freeze-dried tablets, instant tablets, jams, etc. products and the development of cosmetics, resulting in the production of a large number of processing by-products - lemon pomace
It is difficult to develop and utilize lemon pomace, and it is usually discarded or burned directly, resulting in a huge waste of resources and environmental pollution

Method used

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  • A method for extracting limonin in lemon peel residue with guanidinium salt ionic liquid two-phase aqueous phase
  • A method for extracting limonin in lemon peel residue with guanidinium salt ionic liquid two-phase aqueous phase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Randomly purchased lemon juice waste from a lemon processing enterprise—lemon peel pomace as raw material, with a water content of (88.8±3.17)%. The following methods were used to prepare limonin:

[0031] (1) Drying and pulverizing of lemon zest: heating lemon zest at 40-45° C. to dry moisture, and the heating time is until the moisture content of lemon zest drops to 20%. Subsequently, grind and pulverize the dried skin dregs with a pulverizer, and filter through a filter equipped with a 100-120 mesh screen for subsequent use.

[0032] (2) Enzymolysis: preparation of solid compound enzyme, solid lysozyme, solid cellulase, solid 6-phosphogluconolactonase, solid papain, solid citrate lyase and solid ketose diphosphate with a purity of more than 99% The lyase is mixed according to the mass ratio of 0.5:1.5:3:4:0.5:0.5, and dissolved with 200 times the mass of sterile distilled water.

[0033]Add the lemon zest powder screened in the step (1) to an ethanol solution with a...

Embodiment 2

[0041] Randomly purchased lemon juice waste from a lemon processing enterprise—lemon peel pomace as raw material, with a water content of (88.8±3.17)%. The following methods were used to prepare limonin:

[0042] (1) Drying and pulverizing of lemon zest: heating lemon zest at 40-45° C. to dry moisture, and the heating time is until the moisture content of lemon zest drops to 20%. Subsequently, grind and pulverize the dried skin dregs with a pulverizer, and filter through a filter equipped with a 100-120 mesh screen for subsequent use.

[0043] (2) Enzymolysis: preparation of solid compound enzyme, solid lysozyme, solid cellulase, solid 6-phosphogluconolactonase, solid papain, solid citrate lyase and solid ketose diphosphate with a purity of more than 99% The lyase is mixed according to the mass ratio of 0.5:1.5:3:4:0.5:0.5, and dissolved with 200 times the mass of sterile distilled water.

[0044] Add the lemon zest powder screened in the step (1) to an ethanol solution with ...

Embodiment 3

[0052] Randomly purchased lemon juice waste from a lemon processing enterprise—lemon peel pomace as raw material, with a water content of (88.8±3.17)%. The following methods were used to prepare limonin:

[0053] (1) Drying and pulverizing of lemon zest: heating lemon zest at 40-45° C. to dry moisture, and the heating time is until the moisture content of lemon zest drops to 20%. Subsequently, grind and pulverize the dried skin dregs with a pulverizer, and filter through a filter equipped with a 100-120 mesh screen for subsequent use.

[0054] (2) Enzymolysis: preparation of solid compound enzyme, solid lysozyme, solid cellulase, solid 6-phosphogluconolactonase, solid papain, solid citrate lyase and solid ketose diphosphate with a purity of more than 99% The lyase is mixed according to the mass ratio of 0.5:1.5:3:4:0.5:0.5, and dissolved with 200 times the mass of sterile distilled water.

[0055] Add the lemon zest powder screened in the step (1) to an ethanol solution with ...

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Abstract

The invention provides a method for extracting limonin in lemon peel residues by a guanidine salt ion liquid two-aqueous phase extraction system, wherein the method is specially used for extraction and preparation of the limonin in the lemon peel residues. The method includes the steps: (1) performing drying, crushing, enzymolysis and pressure reduction concentration on the lemon peel residues; (2) building and using a guanidine salt ion liquid two-aqueous phase extraction system; (3) performing re-extraction, pressure reduction concentration, freeze drying and purification on compound organicsolvents. According to the method, raw materials are deep-processed by-products of lemon fruits, the by-products are developed into functional products, agricultural wastes are recycled, profit spaceis wide, and development of circular agriculture and green agriculture can be facilitated. According to the method, compound enzymolysis helps to improve later separation efficiency of the limonin inthe lemon peel residues, the guanidine salt ion liquid two-aqueous phase extraction system has the advantages that preparation is simple, stability is high, extraction effects are good, extracts areeasy to recycle and low in volatility, operation temperature is mild and the like, and extraction of functional biological macromolecules is facilitated.

Description

technical field [0001] The invention relates to a method for extracting limonin in lemon peel residue by using compound enzyme hydrolysis to assist guanidine salt ionic liquid two-aqueous phase, and belongs to the field of comprehensive utilization of by-products of fruit and vegetable processing and extraction of functional substances. Background technique [0002] Recently, with the support of the National Natural Science Foundation of China and the Chongqing Municipal Basic and Frontier Research Program, the researchers studied the process of extracting limonin from lemon peel residue with guanidine salt ionic liquid two-phase aqueous phase, and supplemented by the compound enzyme Hydrolysis technology is used for pretreatment, successfully extracting and enriching limonin, and accumulating relevant data and experience, forming the technical solution of the present invention. [0003] Limonin is a high-molecular terpenoid chemical that exists in large quantities in Rutace...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J73/00
CPCC07J73/008
Inventor 汪开拓代领军张帮奎廖云霞伍冬志蒋永波马莉
Owner 重庆汇达生物科技股份有限公司
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