Preparation method of sample for conveniently and rapidly detecting deoxyribonucleic acid (DNA) cell damage and kit using same
A DNA damage and sample preparation technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems affecting the popularization and application of comet electrophoresis technology, the health threat of test operators, and the easy separation of gels from slides, etc. It can reduce the degree of fluorescence attenuation, improve the success rate of glue laying, and be easy to popularize.
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[0035] The present invention will be further described through examples below, but the embodiments of the present invention are not limited thereto.
[0036] (1) Human liver cancer cells Heptatoma G2 (HepG2) were cultured in DMEM culture medium with 10% fetal bovine serum, 100 U / ml penicillin, and 100 μg / ml streptomycin, at 37°C and 5% CO as usual for adherent cells. 2 cultured in an incubator. When the cells were subcultured, the culture medium was discarded, washed twice with PBS, and the HepG2 cells were digested with 0.25% trypsin, and the cell concentration was adjusted to 1×10 6 Each / ml was inoculated in a 6-well plate and cultivated for 24 hours to 80% confluence, different concentrations of 1-butyl-3-methylimidazolium bromide ([C 8mim]Br) ionic liquid for 24 hours, and a blank control group was set up. After the effect of the drug, wash twice with PBS, collect the cells by centrifugation at 800r / min for 5 minutes, resuspend the cells in 1ml pre-cooled PBS, measure th...
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