A compound velvet antler polypeptide freeze-dried powder and nano-scale buffer microemulsion combined essence for skin beauty and health care and its preparation method

Abstract

The invention relates to the fields of cosmetic for skin beauty, health care, skin aging delay and the like, and particularly relates to combined essence of composite velvet antler polypeptide lyophilized powder and nano-scale buffering microemulsion for skin beauty and health care and a preparation method of the combined essence. The preparation method comprises the following steps of: extracting separated composite velvet antler polypeptide lyophilized powder with high efficiency and natural biological activity from fresh velvet antler, and preparing the combined beauty essence by using the composite velvet antler polypeptide lyophilized powder and nano-scale buffering microemulsion capable of prompting transdermal absorption of functional components at a ratio. Therefore, the combined essence provided by the invention has the advantages of fast transdermal absorption speed of the functional components, long biological activity storage period, high biological use degree, good action effect and safe utilization. Furthermore, the combined essence has a terse preparation process and simple technical operation; not only can the energy and the cost be saved, but also the low-carbon environmental friendliness can be achieved, and an environment is prevented from being polluted; and the combined essence provided by the invention is applicable to industrialized mass production, and popularization and application.

Description

technical field [0001] The invention relates to a combination essence which can be used for skin beauty and health care and skin aging delay and its preparation method. It is a compound velvet polypeptide freeze-dried powder extracted from fresh velvet, with natural biological activity velvet polypeptide and its protective agent as the main functional component, and nano-scale buffer microemulsion that can promote the transdermal absorption of functional components by a certain amount. The combined beauty essence formulated in proportion specifically relates to an essence combined with compound velvet antler polypeptide freeze-dried powder and nano-scale buffer microemulsion for skin beauty and health care and a preparation method thereof. Background technique [0002] 1. Technical background of beauty essence: [0003] Essence is the natural functional ingredients or bioactive substances or skin nutrients extracted, separated and purified from certain animal organs or tiss...

AI Technical Summary

Problems solved by technology

[0010] The shortcomings of the current application of beauty essence: the production of beauty essence is simple, easy to operate, convenient to use, and there are many advantages and advantages in practical application and its effect. However, there are many obvious defects and deficiencies in it.

The deficiencies in the application of beauty essence at present mainly include the following aspects: First, most essences used for skin beauty contain ethanol in different concentrations, which have certain olfactory irritation and skin irritation when used, especially some Ethanol-sensitive skin can easily cause skin allergies and dry skin, and it also has different degrees of irritation to the eyes; second, the role and efficacy of beauty essence mainly depend on the functional ingredients contained or added and their biological activity , but many functional ingredients and their biological activities are easily degraded or hydrolyzed in liquid or solvent, so that the molecular structure of many functional ingredients is destroyed, biological activity is lost, and physiological functions are reduced; third, the frequent opening of beauty essence is easy to volatilize and lose part of the solvent, which reduces the solubility and stability of the functional ingredients, which not only affects the sensory and quality of the product, but also affects the stability and actual effect of the product. Therefore, the essence is usually difficult to preserve and has a short storage period. Too long; fourth, if the beauty essence is not paid attention to hygiene during regular use, it is easy to cause pollution, breed a large number of harmful or pathogenic microorganisms, thereby affecting skin health, and even cause cross-infection; fifth, to promote Functional ingredients and biologically active substances are absorbed through the skin. Some beauty essences add a certain concentration of transdermal penetration-promoting agents, and some transdermal penetration-promoting agents are more likely to cause skin allergies or irritations, and excessive use may even have certain biological effects. Toxicity (such as DMSO, etc.); Sixth, most of the beauty essences are water-soluble substances, even if some oil-soluble substances are added, some emulsifiers or co-emulsifiers need to be added to make them evenly dispersed and completely dissolved, but It is also difficult to maintain the long-term stability of the essence even if some emulsifiers or co-emulsifiers are added, and some emulsifiers or co-emulsifiers have been used to cause skin allergies; The solvent itself does not have the effect of promoting the transdermal penetration and absorption of functional ingredients. Even if people artificially add some so-called skin penetration enhancers in the essence, their actual effect of promoting transdermal penetration is very limited, especially because of the skin barrier structure. The histological characteristics of the skin strictly restrict the passage of some exogenous substances, including proteins, water, sugar, lipids, vitamins, inorganic ions or cosmetic functional ingredients, and most functional ingredients cannot or are difficult to pass through the intact skin The stratum corneum barrier plays its due biological role, therefore, the functional ingredients and bioactive substances added to the serum have low bioavailability, limited biological role and use effect

[0011] The shortcomings of the current application of velvet polypeptide: velvet polypeptide has a wide range of biological effects and application fields as a biologically active functional ingredient, but velvet polypeptide is currently mainly used in medical research and some clinical fields, and has not yet been used in skin beauty care and Formal product applied in delaying skin aging

Moreover, if the velvet antler polypeptide prepared by the existing method is used in skin beauty care and skin aging delay products, there are still certain limitations and deficiencies.

The main manifestations are: (1) Although the velvet polypeptide obtained by traditional heating, strong acid or enzymatic proteolysis of fresh velvet antler has a high polypeptide content and a high yield, heating, strong acid or enzymatic proteolysis not only waste more energy and pollute the environment, and the obtained velvet polypeptide has lost its natural biological activity to a large extent, its biological action and physiological function are limited in actual application, and it is difficult to maximize and achieve the application effect that velvet polypeptide should have; (2) using At present, the velvet antler polypeptide obtained by extraction and separation by the conventional acid-alcohol method retains more molecular conformation and natural characteristics than the traditional method, and its biological activity is also improved. There are many insoluble impurities and corticosteroid hormones and sex hormones, which may have skin irritation and allergic risks and side effects when directly used in skin care and skin aging; (3) further purification by column chromatography or high performance liquid phase, although Antler polypeptides with better molecular weight distribution uniformity, higher purity, and better biological activity can be obtained, but the yield of antler polypeptides obtained by this method is not only very low, the operation is cumbersome, special equipment is required, the application cost is high, and the price is expensive. Moreover, this method is difficult for industrial expansion and scale production, and it cannot be widely used; in addition, this method completely eliminates other ingredients, including functional ingredients with synergistic effects, so the actual application effect is also very limited; (4) pilose antler polypeptide in liquid It is not easy to preserve, easy to degrade, and its biological activity is greatly reduced. The best solution is to use freeze-dried powder supplemented with protective agents and synergists

[0012] The shortcomings of the current application of microemulsions: Although there are microemulsion products, some of which may have a particle size of nanometers, the current microemulsions, although their formulations and proportions are not the same, especially their water Use deionized water or distilled water for both phases. Although this is crucial to the formation of the entire microemulsion, the use of distilled water as the water phase to prepare the microemulsion has no buffering and stabilizing effect on its pH value, especially for some microemulsions used for skin beauty, anti-aging, Functional beauty cosmetics such as regenerative repair and promotion of skin cell proliferation have higher requirements on the stability of pH value, especially active peptides and cytokine beauty cosmetics used to promote the proliferation of epidermal and dermal cells, not only need the best pH value environment, and stable pH conditions are needed to be more conducive to the growth and proliferation of skin cells; not only that, the survival, growth, division, and proliferation of skin cells also require a certain ion concentration and osmotic pressure, and these, only the original used The aqueous phase (distilled water) cannot be possessed, and it is also the defect and deficiency of its application

Images