Method for efficiently preparing low-molecular-weight fish skin collagen peptide through enzyme method

Abstract

The invention relates to a method for efficiently preparing low-molecular-weight fish skin collagen peptide through an enzyme method. The method comprises the following steps: (1), after preprocessed fish skin is smashed, adding MCP-01 enzyme liquid and carrying out enzymolysis, so as to obtain reaction liquid; (2), carrying out enzyme inactivation on the reaction liquid obtained in the step (1), then carrying out solid-liquid separation and purifying, so as to obtain the low-molecular-weight fish skin collagen peptide. The low-molecular-weight fish skin collagen peptide prepared through the method provided by the invention has the advantages of low molecular weight, high peptide content, high product yield and the like, thereby having higher water solubility and tissue absorbability, and an excellent commercial application prospect.

Description

Technical field [0001] The invention relates to an enzymatic method for efficiently preparing low molecular weight fish skin collagen peptides, in particular to a method for efficiently preparing low molecular weight fish skin collagen peptides by using marine collagenase MCP-01, which belongs to the technical field of biotechnology. Background technique [0002] my country’s fishery industry has a long history. The output of aquatic products accounts for 1 / 3 of the world’s. It has 1 / 4 of the world’s fishing boats, 1 / 6 of the catch, and nearly 70% of the aquaculture output. The country has about 14 million fishery labors, 7.8 million hectares of farming area, 1 million fishing boats, and fishery output value of 675.2 billion yuan. In 2010, my country’s aquatic product output was 53.73 million tons, accounting for about 30% of global output. As a large aquaculture country, my country accounts for more than 70% of the world's aquaculture production. According to statistics from t...

AI Technical Summary

Problems solved by technology

However, there is no report on cold-adapted collagenase for preparing fish skin collagen peptides by enzymatic hydrolysis of fish skin, and there is no commercialized cold-adapted fish skin collagenase

[0004] At present, most of the commercialized collagen peptides on the market are prepared from a variety of commercial enzyme preparations to enzymolyze collagen materials. There are two common problems: (1) the molecular weight range is widely distributed and the molecular weight of the peptide segment is relatively high. From 1,000 to 10,000, it cannot meet the market demand for small peptide products with smaller molecular weight and uniform distribution, and studies have shown that short peptide chains of 2 to 5 amino acids can directly enter the blood through two types of peptide transport carriers, namely the average molecular weight Small peptides with low average molecular weight are absorbed faster, and short peptides with high average molecular weight are significantly affected by the gastric digestion process, that is, the higher the molecular weight, the water solubility and tissue absorbability of small peptides will be affected to varying degrees

(2) The product yield of enzymatically hydrolyzing fish skin to prepare collagen peptides is low. After the enzymolysis process ends, a large amount of unenzymolyzed fish skin substrates remain, resulting in a large amount of waste of fish skin raw materials and increasing production costs. Seriously reduced business efficiency

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