A method for increasing the yield of low molecular weight soybean peptide
A low-molecular-weight soybean peptide technology, which is applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of high molecular weight of soybean peptide products, insufficient clarity and transparency of the solution, and uneven distribution, so as to achieve clear and bright solutions. The effect of high peptide content and low molecular weight
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Embodiment 1
[0021] A kind of method that the present embodiment provides to improve the yield of low molecular weight soybean peptide comprises the following steps:
[0022] (1) Take soybean protein isolate, add water, after the soybean protein isolate is fully dissolved, adjust the pH of the soybean protein isolate solution to be 6;
[0023] (2) After sterilizing the soybean protein isolate solution obtained in step (1), add a compound protease for enzymolysis to obtain an enzymolysis solution. The dosage of the compound protease is 5‰ of the weight of the soybean protein isolate; The sugar content of the liquid is 5BX, and the pH value is 6;
[0024] (3) After treating the enzymolysis solution obtained in step (2) with microfiltration membrane, add water to wash the filtrate, and collect the filtered soybean peptide mixture; wherein the sugar content of the obtained filtrate is 4BX, and the water in the cleaning process The weight ratio with the filtrate is 1:1;
[0025] (4) The soybe...
Embodiment 2
[0028] A kind of method that the present embodiment provides to improve the yield of low molecular weight soybean peptide comprises the following steps:
[0029] (1) Take soybean protein isolate, add water, after the soybean protein isolate is fully dissolved, adjust the pH of the soybean protein isolate solution to be 8;
[0030] (2) After sterilizing the soybean protein isolate solution obtained in step (1), add a compound protease to carry out enzymolysis to obtain an enzymolysis solution. The dosage of the compound protease is 20‰ of the weight of the soybean protein isolate; The sugar content of the liquid is 10BX, and the pH value is 8;
[0031] (3) After treating the enzymolysis solution obtained in step (2) with microfiltration membrane, add water to wash the filtrate, and collect the filtered soybean peptide mixture; wherein the sugar content of the obtained filtrate is 10BX, and the water in the cleaning process The weight ratio with the filtrate is 1:5;
[0032] (...
Embodiment 3
[0035] A kind of method that the present embodiment provides to improve the yield of low molecular weight soybean peptide comprises the following steps:
[0036] (1) Take soybean protein isolate, add water, after the soybean protein isolate is fully dissolved, adjust the pH of the soybean protein isolate solution to be 8;
[0037] (2) After sterilizing the soybean protein isolate solution obtained in step (1), add a compound protease for enzymolysis to obtain an enzymolysis solution. The dosage of the compound protease is 30‰ of the weight of the soybean protein isolate; The sugar content of the liquid is 18BX, and the pH value is 8;
[0038] (3) After treating the enzymolysis solution obtained in step (2) with microfiltration membrane, add water to wash the filtrate, and collect the filtered soybean peptide mixture; wherein the sugar content of the obtained filtrate is 15BX, and the water in the cleaning process The weight ratio with the filtrate is 1:15;
[0039] (4) The s...
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