The invention discloses a
processing method of
plasma room-temperature transportation, wherein
plasma is separated from maternal
peripheral blood, put into a sterile
nuclease-free device, sealed, andthen can be transported at
room temperature. After transportation at the
room temperature, the original
free DNA concentration,
free DNA fragment distribution, fetal
free DNA percentage concentration,sequencing fragment
length distribution, and
chromosome Z detection value of the
plasma are all equivalent to those of the plasma stored at ultra-low temperature (minus 70 DEG C), and the plasma transported at the
room temperature is suitable for detecting the fetal free
DNA and noninvasive prenatal testing (NIPI) products. The plasma room-temperature transportation method for detecting fetal free
DNA, disclosed by the invention, does not need a special device and does not need to add a protective agent additionally, sample transportation of the plasma can be expanded to normal-temperature transportation, the method improves the problems of inconvenience and high
transportation cost of plasma
cold chain transportation, and can avoid the embarrassment of overtime transportation of a wholeblood sample and
hemolysis due to a transport
mechanical force, and a novel, effective and convenient alternative method is provided for the existing maternal
peripheral blood processing mode and plasma transportation mode.