The invention discloses a photoelectrochemical
ethinylestradiol immunosensor based on two-dimensional multi-
metal composite nanomaterial, preparation and application thereof. According to the preparation method, firstly electrodeposition process is employed to modify the two-dimensional nanomaterial g-C3N4 on ITO conductive glass, then a hydrothermal process is employed for in situ growth of
cerium cobalt hydroxide, and then a g-C3N4 loaded
boron doped
cerium cobalt oxide Bi@CexCo1-xO3 / g-C3N4 modified
working electrode can be prepared. Finally, the good
biocompatibility and large specific surface area of the material are utilized for loading the
ethinylestradiol antobody. During detection, the
boron doped
cerium cobalt oxide Bi@CexCo1-xO3 can catalyze L-
ascorbic acid-2-
trisodium phosphate salt AAP for in situ production of L-
ascorbic acid AA, thus providing an
electron donor for photoelectric detection, and then by means of the influence caused by specific quantitative binding of the
antibody and
antigen on
electron transmission ability, the
photocurrent intensity decreases along with the increasing of
antigen binding amount, and ultimately construction of the
photoelectric sensor for detection of
ethinylestradiol by an unmarked photoelectrochemical method can be achieved.