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A photoelectrochemical diethylstilbestrol biosensor based on boron-doped multi-element metal oxides and its preparation and application

A biosensor, diethylstilbestrol technology, applied in the direction of material electrochemical variables, etc., can solve the problem of reducing charge carrier recombination, and achieve the effect of improving detection sensitivity and good conductivity

Active Publication Date: 2019-08-27
山东利源康赛环境咨询有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the heterojunction is also sufficiently induced to sterically effectively reduce the recombination of charge carriers during the coupled hybridization process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This embodiment provides a photoelectrochemical diethylstilbestrol biosensor based on boron-doped multi-element metal oxides and its preparation and application. The preparation method includes the following steps:

[0049] S11: Use ITO conductive glass as the working electrode, platinum wire electrode as the counter electrode, and g-C 3 N 4 The mixed solution with graphene oxide is the electrolyte. In the two-electrode system, cyclic voltammetry is adopted, the voltage range is -1.4 ~ 0.4V, the sweep rate is 60mV / s, and the electrodeposition is 20 cycles, and the deposited electrode is used Wash with deionized water to obtain g-C 3 N 4 The modified working electrode; where, g-C 3 N 4 The configuration method of the mixed solution with graphene oxide is: add 5mg of g-C 3 N4 Mix with 0.1mg of graphene oxide, add 10mL of deionized water, then stir for 10min, and then conduct ultrasonic treatment in a water bath for 1 hour to obtain g-C 3 N 4 and graphene oxide mixe...

Embodiment 2

[0062] This embodiment provides a diethylstilbestrol photoelectrochemical sensor and its preparation and application. The preparation method includes the following steps:

[0063] S11: Use ITO conductive glass as the working electrode, platinum wire electrode as the counter electrode, and g-C 3 N 4 The mixed solution with graphene oxide is the electrolyte. In the two-electrode system, cyclic voltammetry is adopted, the voltage range is -1.4 ~ 0.4V, the scan rate is 80mV / s, and the electrodeposition is 15 laps. The deposited electrode is used Wash with deionized water to obtain g-C 3 N 4 The modified working electrode; where, g-C 3 N 4 The configuration method of the mixed solution with graphene oxide is: add 5mg of g-C 3 N 4 Mix with 0.3mg of graphene oxide, add 10mL of deionized water, then stir for 10min, and then conduct ultrasonic treatment in a water bath for 1 hour to obtain g-C 3 N 4 and graphene oxide mixed solution.

[0064] S12: the g-C obtained in step S11 ...

Embodiment 3

[0075] This embodiment provides a diethylstilbestrol photoelectrochemical sensor and its preparation and application. The preparation method includes the following steps:

[0076] S11: Use ITO conductive glass as the working electrode, platinum wire electrode as the counter electrode, and g-C 3 N 4 The mixed solution with graphene oxide is the electrolyte. In the two-electrode system, the cyclic voltammetry is adopted, the voltage range is -1.4~0.4V, the scan rate is 100mV / s, and the electrodeposition is 11 laps, and the deposited electrode is used Wash with deionized water to obtain g-C 3 N 4 The modified working electrode; where, g-C 3 N 4 The configuration steps of the mixed solution of graphene oxide and graphene oxide are as follows: 5 mg of g-C 3 N 4 Mix with 0.5mg of graphene oxide, add 10mL of deionized water, then stir for 10min, and then conduct ultrasonic treatment in a water bath for 1 hour to obtain g-C 3 N 4 and graphene oxide mixed solution.

[0077] S1...

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Abstract

The invention discloses a photo-electrochemical diethylstilbestrol biosensor constructed based on a boron-doped poly-metallic oxide as well as preparation and application thereof. In a preparation method, firstly, a two-dimension nano material g-C3N4 is modified on ITO conductive glass by adopting an electro-deposition method; then, an iron cobalt hydroxide is subjected to in-situ growth by adopting a hydrothermal method, and further, a working electrode modified by a g-C3N4-loaded boron-doped iron cobalt oxide Bi@NixFe1-xO3 / g-C3N4 is obtained; finally, a diethylstilbestrol antibody is loaded by utilizing good biocompatibility and large specific surface area of the material. During detection, as the boron-doped iron cobalt oxide Bi@NixFe1-xO3 / g-C3N4 can catalyze L-ascorbic acid-2-trisodium phosphate AAP, L-ascorbic acid AA is generated in situ, and further, an electron donor is provided for photoelectric detection; by utilizing the effect of specific quantitative binding of the antibody and an antigen on electronic transmission capacity, the photocurrent strength is correspondingly reduced, and the construction of a photoelectric sensor for detecting diethylstilbestrol by adopting an unmarked photo-electrochemical method is finally realized.

Description

technical field [0001] The present invention relates to the technical field of preparation of novel nano-functional materials and detection of biochemical sensors, in particular to a photoelectrochemical sensor that can be used for the detection of diethylstilbestrol and its preparation and application, and in particular to a photoelectrochemical sensor based on boron-doped multi-element metal oxides. Diethylstilbestrol biosensor and its preparation and application. Background technique [0002] Environmental Estrogens (Environmental Estrogens), also known as environmental hormones, are a class of environmental endocrine disrupting substances that have estrogenic effects or antagonistic androgen effects in the environment. These substances widely exist in rivers, soil, air and agricultural products, and can enter human or animal bodies through the food chain or direct contact, interfere with the endocrine system of the body, destroy the normal hormone levels in the plasma, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/36G01N27/38
Inventor 高丕成姜艳艳周大锋王亚男杨青青黎荣霞魏晓鹏王晓东翟振旭
Owner 山东利源康赛环境咨询有限责任公司
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