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A preparation method of photoelectrochemical chloramphenicol biosensor based on two-dimensional nano-optoelectronic material

A biosensor, two-dimensional nanotechnology, applied in electrochemical variables of materials, material analysis by electromagnetic means, scientific instruments, etc. Widen the range of photosensitive wavelengths, increase photocatalytic activity, and save time

Inactive Publication Date: 2019-04-05
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In addition, the photogenerated electron-hole pairs of a single titanium dioxide nanomaterial are easy to recombine, which leads to the weakening of the photoelectric signal, and the poor conductivity of titanium dioxide also limits the sensitivity of photoelectrochemical sensors constructed from a single titanium dioxide nanomaterial. application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Mn-TiO 2 / MoS 2 preparation of

[0042] (1) Add 0.6 g of molybdenum disulfide powder and 0.2 mmol of manganese salt to 3 mL of n-butyllithium solution, and stir for 12 hours under nitrogen protection at 60 °C to obtain the reacted solution;

[0043] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 60 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain manganese Intercalated molybdenum disulfide nanomaterials;

[0044] (3) Take 500 mg of manganese-intercalated molybdenum disulfide nanomaterials prepared in step (2) and add them to 5 mL of tetrabutyl titanate. After stirring for 1 hour, slowly add 0.5 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 160°C for 18 hours;

[0045] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute ethanol...

Embodiment 2

[0050] Example 2 Mn-TiO 2 / MoS 2 preparation of

[0051] (1) Add 0.6 g of molybdenum disulfide powder and 1.0 mmol of manganese salt to 5 mL of n-butyllithium solution, and stir for 24 hours under nitrogen protection at 30 °C to obtain the reacted solution;

[0052] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 30 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain manganese Intercalated molybdenum disulfide nanomaterials;

[0053] (3) Add 200 mg of manganese-intercalated molybdenum disulfide nanomaterials prepared in step (2) into 5 mL of tetrabutyl titanate, stir for 1 hour, then slowly add 0.6 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 180°C for 20 hours;

[0054] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum...

Embodiment 3

[0059] Example 3 Preparation method of photoelectrochemical chloramphenicol biosensor

[0060] (1) Use an ITO conductive glass with a width of 1 cm and a length of 4 cm as a working electrode, and drop-coat 8 µL of Mn-TiO on the surface of the electrode 2 / MoS 2 Sol, dry at room temperature;

[0061] (2) Wash the electrode obtained in step (1) with the buffer solution PBS, continue to drop-coat 8 µL of 10 µg / mL chloramphenicol antibody solution on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0062] (3) Wash the electrode obtained in step (2) with PBS, continue to drip-coat 8 µL of bovine serum albumin solution with a concentration of 100 µg / mL on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0063] (4) Wash the electrode obtained in step (3) with PBS, continue to drip-coat 6 µL of alkaline phosphatase solution with a concentration of 20 µg / mL on the surface of the electrode, and store it in a refrigerator at ...

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Abstract

The invention discloses a preparation method of a photoelectrochemical chloramphenicol biosensor based on a two-dimensional nano photoelectric material. It belongs to the technical field of new nanometer functional materials and biosensors. The present invention first prepares a novel two-dimensional nano-optoelectronic material, that is, a two-dimensional nano-composite material Mn-TiO in which manganese-doped titanium dioxide nano-squares are in-situ compounded with molybdenum disulfide. 2 / MoS 2 , using the good biocompatibility and large specific surface area of ​​the material, load chloramphenicol antibody and immobilize alkaline phosphatase. When performing detection, since alkaline phosphatase can catalyze L-ascorbic acid-2-phosphate The trisodium salt AAP produces L-ascorbic acid AA in situ, and then provides electron donors for photoelectric detection, and then uses the effect of specific quantitative binding of antibodies and antigens on the electron transport ability, so that the photocurrent intensity is correspondingly reduced, and finally realized the use of Construction of a biosensor for the detection of chloramphenicol by a label-free photoelectrochemical method.

Description

technical field [0001] The invention relates to a preparation method of a photoelectrochemical chloramphenicol biosensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Chloramphenicol is an antibiotic produced by Streptomyces venezuela. It is a broad-spectrum antibacterial antibiotic and is often used as a veterinary drug. However, the human body is more sensitive to chloramphenicol than animals, especially the drug metabolism function of infants and young children is not perfect. Excessive chloramphenicol can cause fatal "gray baby syndrome" reactions, and even cause human aplastic impairment in severe cases sexual anemia. [0003] At present, the methods for detecting chloramphenicol mainly include chromatography and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need professional training before they can perform detection. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/416G01N27/36
CPCG01N27/36G01N27/416
Inventor 张勇杜斌马洪敏吴丹范大伟
Owner UNIV OF JINAN
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