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Photoelectrochemical ethinylestradiol immunosensor based on two-dimensional multi-metal composite nanomaterial, preparation and application thereof

A technology of composite nanomaterials and immune sensors, applied in the direction of material electrochemical variables, scientific instruments, analytical materials, etc., can solve the problem of reducing charge carrier recombination, and achieve the effect of improving detection sensitivity and good conductivity

Active Publication Date: 2017-11-17
山东利源康赛环境咨询有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, the heterojunction is also sufficiently induced to sterically effectively reduce the recombination of charge carriers during the coupled hybridization process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This embodiment provides a photoelectrochemical ethinyl estradiol immunosensor based on two-dimensional multi-component metal composite nanomaterials and its preparation and application. The preparation method includes the following steps:

[0049] S11: Use ITO conductive glass as the working electrode, platinum wire electrode as the counter electrode, and g-C 3 N 4 The mixed solution with graphene oxide is the electrolyte. In the two-electrode system, cyclic voltammetry is adopted, the voltage range is -1.4 ~ 0.4V, the sweep rate is 60mV / s, and the electrodeposition is 20 cycles, and the deposited electrode is used Wash with deionized water to obtain g-C 3 N 4 The modified working electrode; where, g-C 3 N 4 The configuration method of the mixed solution with graphene oxide is: add 5mg of g-C 3 N4 Mix with 0.1mg of graphene oxide, add 10mL of deionized water, then stir for 10min, and then conduct ultrasonic treatment in a water bath for 1 hour to obtain g-C 3 N ...

Embodiment 2

[0062] This embodiment provides a photoelectrochemical sensor for ethinyl estradiol and its preparation and application. The preparation method includes the following steps:

[0063] S11: Use ITO conductive glass as the working electrode, platinum wire electrode as the counter electrode, and g-C 3 N 4 The mixed solution with graphene oxide is the electrolyte. In the two-electrode system, cyclic voltammetry is adopted, the voltage range is -1.4 ~ 0.4V, the scan rate is 80mV / s, and the electrodeposition is 15 laps. The deposited electrode is used Wash with deionized water to obtain g-C 3 N 4 The modified working electrode; where, g-C 3 N 4 The configuration method of the mixed solution with graphene oxide is: add 5mg of g-C 3 N 4 Mix with 0.3mg of graphene oxide, add 10mL of deionized water, then stir for 10min, and then conduct ultrasonic treatment in a water bath for 1 hour to obtain g-C 3 N 4 and graphene oxide mixed solution.

[0064] S12: the g-C obtained in step S...

Embodiment 3

[0075] This embodiment provides a photoelectrochemical sensor for ethinyl estradiol and its preparation and application. The preparation method includes the following steps:

[0076] S11: Use ITO conductive glass as the working electrode, platinum wire electrode as the counter electrode, and g-C 3 N 4 The mixed solution with graphene oxide is the electrolyte. In the two-electrode system, the cyclic voltammetry is adopted, the voltage range is -1.4~0.4V, the scan rate is 100mV / s, and the electrodeposition is 11 laps, and the deposited electrode is used Wash with deionized water to obtain g-C 3 N 4 The modified working electrode; where, g-C 3 N 4 The configuration steps of the mixed solution of graphene oxide and graphene oxide are as follows: 5 mg of g-C 3 N 4 Mix with 0.5mg of graphene oxide, add 10mL of deionized water, then stir for 10min, and then conduct ultrasonic treatment in a water bath for 1 hour to obtain g-C 3 N 4 and graphene oxide mixed solution.

[0077]...

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Abstract

The invention discloses a photoelectrochemical ethinylestradiol immunosensor based on two-dimensional multi-metal composite nanomaterial, preparation and application thereof. According to the preparation method, firstly electrodeposition process is employed to modify the two-dimensional nanomaterial g-C3N4 on ITO conductive glass, then a hydrothermal process is employed for in situ growth of cerium cobalt hydroxide, and then a g-C3N4 loaded boron doped cerium cobalt oxide Bi@CexCo1-xO3 / g-C3N4 modified working electrode can be prepared. Finally, the good biocompatibility and large specific surface area of the material are utilized for loading the ethinylestradiol antobody. During detection, the boron doped cerium cobalt oxide Bi@CexCo1-xO3 can catalyze L-ascorbic acid-2-trisodium phosphate salt AAP for in situ production of L-ascorbic acid AA, thus providing an electron donor for photoelectric detection, and then by means of the influence caused by specific quantitative binding of the antibody and antigen on electron transmission ability, the photocurrent intensity decreases along with the increasing of antigen binding amount, and ultimately construction of the photoelectric sensor for detection of ethinylestradiol by an unmarked photoelectrochemical method can be achieved.

Description

technical field [0001] The invention relates to the technical field of preparation of novel nano functional materials and detection of biochemical sensors, in particular to a photoelectrochemical sensor which can be used for detection of ethinyl estradiol and its preparation and application, in particular to a photoelectric sensor based on two-dimensional multi-element metal composite nanomaterials Chemical ethinyl estradiol immunosensor and its preparation and application. Background technique [0002] Environmental Estrogens (Environmental Estrogens), also known as environmental hormones, are a class of environmental endocrine disrupting substances that have estrogenic effects or antagonistic androgen effects in the environment. These substances widely exist in rivers, soil, air and agricultural products, and can enter human or animal bodies through the food chain or direct contact, interfere with the endocrine system of the body, destroy the normal hormone levels in the p...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327G01N33/74
Inventor 姜艳艳周大锋高丕成杨青青王亚男王晓东魏晓鹏王宏宇
Owner 山东利源康赛环境咨询有限责任公司
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