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A preparation method of photoelectrochemical internal phosphorus sensor based on cobalt-doped two-dimensional nano-optoelectronic material

A two-dimensional nanometer and photoelectrochemical technology, which is applied to the electrochemical variables of materials, material analysis through electromagnetic means, and scientific instruments, can solve the problems of low sensitivity of photoelectrochemical sensors, unfavorable practical applications, and weakened photoelectric signals. Broaden the range of photosensitive wavelengths, increase photocatalytic activity, and save time

Inactive Publication Date: 2019-03-12
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In addition, the photogenerated electron-hole pairs of a single titanium dioxide nanomaterial are easy to recombine, which leads to the weakening of the photoelectric signal, and the poor conductivity of titanium dioxide also limits the sensitivity of photoelectrochemical sensors constructed from a single titanium dioxide nanomaterial. application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Co-TiO 2 / MoS 2 preparation of

[0042] (1) Add 0.6 g of molybdenum disulfide powder and 0.2 mmol of cobalt salt to 3 mL of n-butyllithium solution, and stir for 12 hours under nitrogen protection at 60 °C to obtain the reacted solution;

[0043] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 60 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain cobalt Intercalated molybdenum disulfide nanomaterials;

[0044] (3) Add 500 mg of cobalt-intercalated molybdenum disulfide nanomaterials prepared in step (2) into 5 mL of tetrabutyl titanate, stir for 1 hour, slowly add 0.5 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 160°C for 18 hours;

[0045] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum-dried at 50 °...

Embodiment 2

[0050] Example 2 Co-TiO 2 / MoS 2 preparation of

[0051] (1) Add 0.6 g of molybdenum disulfide powder and 1.0 mmol of cobalt salt into 5 mL of n-butyllithium solution, and stir for 24 hours under nitrogen protection at 30 °C to obtain the reacted solution;

[0052] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 30 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain cobalt Intercalated molybdenum disulfide nanomaterials;

[0053] (3) Add 200 mg of cobalt-intercalated molybdenum disulfide nanomaterials prepared in step (2) into 5 mL of tetrabutyl titanate, stir for 1 hour, then slowly add 0.6 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 180°C for 20 hours;

[0054] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum-dried ...

Embodiment 3

[0059] Example 3 Preparation method of photoelectrochemical systemic phosphorus sensor

[0060] (1) ITO conductive glass with a width of 1 cm and a length of 4 cm was used as a working electrode, and 8 µL of Co-TiO was drop-coated on the surface of the electrode 2 / MoS 2 Sol, dry at room temperature;

[0061] (2) Wash the electrode obtained in step (1) with buffer solution PBS, continue to drop-coat 8 µL of 10 µg / mL demeton antibody solution on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0062] (3) Wash the electrode obtained in step (2) with PBS, continue to drip-coat 8 µL of bovine serum albumin solution with a concentration of 100 µg / mL on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0063] (4) Wash the electrode obtained in step (3) with PBS, continue to drip-coat 6 µL of alkaline phosphatase solution with a concentration of 20 µg / mL on the surface of the electrode, and store it in a refrigerator at 4 °...

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Abstract

The invention discloses a preparation method of a photoelectrochemical systemic phosphorus sensor. It belongs to the technical field of new nanometer functional materials and biosensors. The present invention firstly prepares a novel two-dimensional nano-optoelectronic material—cobalt-doped two-dimensional nano-optoelectronic material, that is, a two-dimensional nano-composite material Co-TiO2 / MoS2 in which cobalt-doped titanium dioxide nano-squares are in-situ compounded with molybdenum disulfide. The material has good biocompatibility and large specific surface area, loaded with Demeton antibody, immobilized with alkaline phosphatase, when performing detection, because alkaline phosphatase can catalyze L-ascorbic acid-2-trisodium phosphate The salt AAP produces L-ascorbic acid AA in situ, and then provides an electron donor for photoelectric detection, and then uses the effect of the specific quantitative binding of antibody and antigen on the electron transport ability, so that the photocurrent intensity is correspondingly reduced, and finally realizes the use of label-free Construction of photosensors for the detection of systemic phosphorus by photoelectrochemical methods.

Description

technical field [0001] The invention relates to a preparation method of a photoelectrochemical systemic phosphorus sensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Demeton is an organophosphorus pesticide with a mercaptan odor and is a highly toxic pesticide. In soil, systemic phosphorus can migrate slightly to the deep layer of soil through water leaching. The systemic phosphorus in the soil can be absorbed by the plant roots and enter the plant body. After people eat such plants or plants containing their residues by mistake, Demeton can enter the human body through the digestive tract, respiratory tract and intact skin and mucous membranes, causing nausea, vomiting, headache, diarrhea, and general weakness. Symptoms, long-term consumption or excessive consumption can cause cancer. [0003] At present, the methods for detecting demeton mainly include chromatography and mass spectrometry. Such...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327G01N33/531G01N33/543
CPCG01N27/305G01N27/3278G01N33/531G01N33/54346G01N2496/45
Inventor 张勇李燕王欢匡轩姜娜
Owner UNIV OF JINAN
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