42 results about "Single-use bioreactor" patented technology

A composite sensor assembly for monitoring bio-processes which is suitable for use with a polymeric bioprocess vessel or with downstream equipment, and comprises:i) a port comprising a high surface tension thermoplastic having a hollow tubular portion and a base plate portion, the base plate portion being fusibly sealable to the bioreactor vessel at a hole in the wall thereof;ii) a generally opaque polymeric monitoring sensor assembly including electrical, and / or optical measurement components. The sensor assembly fits inside the bore of the hollow tubular portion of the port, and is adhesively retained therein.

A composite sensor assembly for monitoring bio-processes which is suitable for use with a polymeric bioprocess vessel or with downstream equipment, and comprises:i) a port comprising a high surface tension thermoplastic having a hollow tubular portion and a base plate portion, the base plate portion being fusibly sealable to the bioreactor vessel at a hole in the wall thereof;ii) a generally opaque polymeric monitoring sensor assembly including electrical, and / or optical measurement components. The sensor assembly tits inside the bore of the hollow tubular portion of the port, and is adhesively retained therein.

A composite sensor assembly for monitoring bio-processes which is suitable for use with a polymeric bioprocess vessel or with downstream equipment, and comprises:i) a port comprising a high surface tension thermoplastic having a hollow tubular portion and a base plate portion, the base plate being fusibly sealable to the bioreactor vessel at a hole in the wall thereof;ii) a generally opaque polymeric monitoring sensor assembly including electrical, and / or optical measurement components. The sensor assembly fits inside the bore of the hollow tubular portion of the port, and is adhesively retained therein.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

The invention provides a pH value detection biological reaction system. The system comprises a bioreactor vessel (51) having a plastic wall (50) and a pH sensor (40, 60, 80, 100, 120) attached to said plastic wall (50). The pH sensor (40, 60, 80, 100, 120) includes a sensor body (82) having a flange (76) sealingly attached to the plastic wall (50). The sensor main body (82) has a reference electrolyte (142) and a first detection element (152) arranged in the reference electrolyte (142). The first detection element (152) is configured to contact both the reference electrolyte (142) and the sample solution (52) within the bioreactor vessel. A second detection element (62, 92, 148) is positionable inside the bioreactor vessel (51). The pH sensor (40, 60, 80, 100, 120) has multiple configurations, including a protective configuration in which the at least one detection element is isolated from the interior of the bioreactor container (51), and the at least one detection element is fluid ground connected to the internal working configuration of the bioreactor vessel (51).