31 results about "Amycolatopsis orientalis" patented technology

Novel polyene polyketides, their pharmaceutically acceptable salts, prodrugs and derivatives have been found to have antibiotic activity. One method for obtaining the compounds is by cultivation of Amycolatopsis orientalis ATCC™ 43491 or a mutant or variant such as the strain IDAC-220604-1. Another method for obtaining the compounds is post-biosynthesis chemical modification of the compounds obtained by cultivation. Novel polynucleotide sequences and encoded proteins for the biosynthesis of the polyene polyketides are also presented.

Disclosed are a ketoreductase mutant which can be used for an efficient production of daunorubicin derivatives, a DNA encoding the mutant, a transformant prepared by introducing the DNA thereinto to produce a daunorubicin derivative, and a process of producing a daunorubicin derivative using the transformant. The ketoreductase mutant has an amino acid sequence in which one amino acid residue or two or more amino acid residues selected from the group consisting of amino acids located at positions corresponding to the 42nd, 149th, 153rd, 270th, and 306th amino acids in the amino acid sequence of a ketoreductase (EvaE) from a chlororemomycin-producing bacterium (Amycolatopsis orientalis) are substituted with another amino acid residues.

The invention discloses a method for preparing A82846B by fermentation. The method is to extract the fermentation culture liquid obtained by fermenting and cultivating Amycolatopsis orientalis, and then carry out alkali degradation on the extracted impurity waste liquid to obtain the alkali degradation liquid. After the alkali degradation solution was added to the fermentation medium, the fermentation was carried out again. By adding different concentrations of impurity alkali degradation solution, the fermentation unit of A82846B was greatly increased, and the components were also significantly improved.

The invention discloses a method for improving the yield of ECO-0501 produced by fermenting Amycolatopsis orientalis, which is achieved by interdicting the Amycolatopsis orientalis to synthesize vancomycin, wherein the synthesis of the interdicted vancomycin comprises interdicting or knocking out vancomycin synthetase genes or vancomycin precursor synthetase genes. The method for improving the yield of the compound ECO-0501 produced by fermenting the Amycolatopsis orientalis interdicts the synthesis of the vancomycin while improving the yield of the ECO-0501, thus the method is particularly favorable for the downstream separation steps.

The invention provides ribosome resistance mutagenesis breeding vancomycin production bacterial strain and an application thereof, specifically the mutagenesis breeding method of mutagenesis bacterial strain of Amycolatopsis orientalis and the application thereof in preparing vancomycin, relating to breeding of microorganism. The method comprises the following steps: inoculating the Amycolatopsisorientalis to Gause-1 agar culture medium panel to be cultured; taking out spore and using sterile water to prepare spore suspension and coating the spore suspension in Gause-1 agar culture medium panel featuring mixing resistance of vancomycin and streptomycin to be cultured; randomly selecting produced single bacterial colony and streak-inoculating the single bacterial colony to the Gause-1 agar culture medium panel to be cultured, carrying out biological activity determination on produced mutagenesis bacterial strain; selecting mutagenesis bacterial strain with inhibition zone diameter thereof being 2mm larger than the inhibition zone diameter of original strain and inoculating the mutagenesis bacterial strain to liquid fermentation medium to be cultured; determining the content of vancomycin in fermentation liquor by HPLC method to verify the obtained vancomycin mutagenesis bacterial strain; subjecting the obtained vancomycin mutagenesis bacterial strain to 5 times of continuous passage stability inspection to obtain the vancomycin production bacterial strain.