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Isoflavone glycoside compound and preparation method thereof

A technology of compounds and eluents, applied in the field of biochemistry, to achieve a good inhibitory effect

Active Publication Date: 2014-04-02
SHANGHAI LAIYI BIOMEDICAL RES & DEV CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Since the discovery and use of penicillin, humans have made a major breakthrough in the fight against microbial infections, but with the continuous use of antibiotics, the resistance of bacteria, fungi and other microorganisms has also continued to increase, which poses a continuous challenge to clinical anti-infection treatment

Method used

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  • Isoflavone glycoside compound and preparation method thereof
  • Isoflavone glycoside compound and preparation method thereof
  • Isoflavone glycoside compound and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 , Fermentation of Amycolatopsis orientalis ATCC 43491

[0045] Take the slant culture of Amycolatopsis orientalis ATCC 43491, inoculate it into the seed medium for cultivation, and cultivate it on a shaker at 28°C and 220r / min for 40 hours to obtain the seed culture solution.

[0046] The seed culture solution was inoculated into the fermentation medium, and cultured on a shaker at 28° C. at a speed of 220 r / min for 120 hours to obtain a fermentation solution.

[0047] Acidify the fermentation broth with 4mol / L hydrochloric acid, adjust the pH to 3-4, and collect the supernatant by centrifugation.

Embodiment 2

[0048] Example 2 , fermentation broth purification

[0049] The centrifugal supernatant that obtains in embodiment 1 is carried out HPLC detection, and the result is as follows figure 1 As shown, then the centrifuged supernatant was purified by HPD100 resin and purified by medium-pressure liquid chromatography to obtain a compound with a purity of 98%. The specific process is as follows:

[0050] A) HPD100 resin primary purification

[0051] The centrifuged supernatant is loaded onto the HPD100 resin column, and ethanol-water solution gradient elution is used to collect the eluate in the range of ethanol volume percentage from 25% to 35%, and then the eluate is concentrated to obtain a crude product concentrate.

[0052] B) the concentrated solution obtained in step A is purified by medium-pressure liquid chromatography technology, and the specific process is as follows:

[0053] The above-mentioned crude product concentrate is loaded on the reversed-phase C-18 column, fir...

Embodiment 3

[0055] Example 3 , LYV09lx01 identification

[0056] The physical and chemical properties of LYV09lx01 are as follows:

[0057] Compound LYV09lx01 is white powder, slightly yellowish, soluble in water, soluble in methanol, insoluble in acetone, butanol, ethyl acetate;

[0058] Mass spectrometry (MS), hydrogen nuclear magnetic resonance ( 1 H NMR), carbon nuclear magnetic resonance ( 13 C NMR) compound LYV09lx01 is measured, and the results are as follows:

[0059] The mass spectrometry detection results of compound LYV09lx01 are as follows image 3 shown, according to image 3 The result of [M+H] + =563, [2M+H] + = 1125, the molecular weight of compound LYV09lx01 was deduced to be 562.

[0060] The carbon NMR spectrum of compound LYV09lx01 is as Figure 4 As shown, the detection results of its proton nuclear magnetic resonance spectrum are as follows Figure 5 As shown, the spectrum resolution results of the C-NMR spectrum and the H-NMR spectrum are shown in Table 2...

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Abstract

The invention provides an isoflavone glycoside compound and a preparation method thereof. The compound provided by the invention is in a structure shown in a structural formula (1) and is obtained by fermenting and culturing amycolatopsis orientalis. The compound provided by the invention has favorable inhibition action on white candida.

Description

technical field [0001] The invention belongs to the field of biochemistry, and specifically relates to an isoflavone glycoside compound and a preparation method thereof. Background technique [0002] Since the discovery and use of penicillin, humans have made a major breakthrough in the fight against microbial infections. However, with the continuous use of antibiotics, the resistance of bacteria, fungi and other microorganisms has also continued to increase, which poses a continuous challenge to clinical anti-infection treatment. [0003] Therefore, finding new antibiotics or compounds with bacteriostatic / bactericidal effects has become an important research topic and has the same urgency, especially in the aspect of antifungal drugs. Contents of the invention [0004] The compound involved in the present invention comes from the fermentation broth of Amycolatopsis orientalis ATCC 43491, which is a component with a relatively low content in the fermentation broth, which i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C12P19/60A61K31/7048A61P31/04C12R1/04
Inventor 殷瑜杨志钧夏兴刘效稳阮丽军李秋爽金文翔杨天魏维戈梅罗敏玉陈代杰
Owner SHANGHAI LAIYI BIOMEDICAL RES & DEV CENT
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