Method for removing fixed uranium by utilizing denitrification and phosphorus accumulation effects of microorganisms

A microbial and uranium-fixing technology, which is applied in the field of remediation of polluted water in uranium mining areas, can solve problems such as sediment instability, reduce potential risks, and increase pH

Active Publication Date: 2021-04-02
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method must rely on the microbial dye cell device, which provides electrons during the reaction, and the precipitate is unstable.

Method used

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  • Method for removing fixed uranium by utilizing denitrification and phosphorus accumulation effects of microorganisms

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Experimental program
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Effect test

Embodiment 1

[0027] The method for removing fixed uranium by utilizing the denitrification and phosphorus accumulation of microorganisms comprises the following steps:

[0028] (1) Strain mixed solution: the activated sludge obtained from the biochemical pool of the anaerobic section of the sewage treatment plant is mixed with sterile water to obtain a mixed solution with bacteria. The concentration of activated sludge in the mixed solution with bacteria is 3g / L, and the dissolved oxygen content of the mixed solution with bacteria is 3mg / L;

[0029] (2) Bacteria culture in mixed solution: put the mixed solution with bacteria obtained in step (1) in LB medium, grow at an ambient temperature of 25+1°C, and grow for 24 hours to obtain bacteria in mixed solution, wherein LB medium is prepared as follows : Distilled water 1L, yeast extract 5g / L, tryptone 10g / L, NaCl 5g / L; trace element formula: (NH 4 ) 6 (MO 7 ) 24 0.1mg / L, H 3 BO 3 1.0mg / L, CoCl 2 1.5mg / L, CuSO 4 0.1mg / L, MnCl 2 0.5mg...

Embodiment 2

[0034] The method for removing fixed uranium by utilizing the denitrification and phosphorus accumulation of microorganisms comprises the following steps:

[0035] (1) Strain mixture: the activated sludge obtained from the biochemical pool of the anaerobic section of the sewage treatment plant is mixed with sterile water to obtain a mixed solution with bacteria. The concentration of activated sludge in the mixed solution with bacteria is 5g / L, and the dissolved oxygen content of the mixed solution with bacteria is 5mg / L;

[0036] (2) Bacteria culture in the mixed solution: put the mixed solution with bacteria obtained in step (1) in LB medium, at an ambient temperature of 25+1°C, and a growth time of 36 hours, to obtain bacteria in the mixed solution, wherein the LB medium is prepared as follows : Distilled water, yeast extract 5.2g / L, tryptone 12g / L, NaCl6g / L; trace element formula: (NH 4 ) 6 (MO 7 ) 24 0.2mg / L,H 3 BO 3 1.2mg / L, CoCl 2 1.52mg / L, CuSO 4 0.2mg / L, MnCl 2 ...

Embodiment 3

[0040] The method for removing fixed uranium by utilizing the denitrification and phosphorus accumulation of microorganisms comprises the following steps:

[0041] (1) Strain mixture: the activated sludge obtained from the biochemical pool of the anaerobic section of the sewage treatment plant is mixed with sterile water to obtain a mixed solution with bacteria. The concentration of activated sludge in the mixed solution with bacteria is 4g / L, and the dissolved oxygen content of the mixed solution with bacteria is 4mg / L;

[0042] (2) Bacteria culture in mixed solution: put the mixed solution with bacteria obtained in step (1) in LB medium, grow at an ambient temperature of 25+1°C, and grow for 48 hours to obtain mixed solution bacteria, wherein the LB medium is prepared as follows : Distilled water, yeast extract 5.1g / L, tryptone 11g / L, NaCl5.5g / L; trace element formula: (NH 4 ) 6 (MO 7 ) 24 0.15mg / L,H 3 BO 3 1.15mg / L, CoCl 2 1.51mg / L, CuSO 4 0.15mg / L, MnCl 2 0.55mg / L...

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Abstract

The invention relates to a method for removing fixed uranium by utilizing denitrification and phosphorus accumulation effects of microorganisms, and belongs to the technical field of uranium mine areapolluted water body remediation. Activated sludge is obtained from a biochemical pool of an anaerobic section of a sewage treatment plant, and sterile water is adopted for mixing to obtain a bacteria-carrying mixed solution; the obtained bacteria-carrying mixed solution is put into an LB culture medium, and grows for 24-48h at the environment temperature of 25+1 DEG C to obtain mixed solution bacteria; the mixed solution bacteria are put into a denitrification and phosphorus accumulation culture medium, and subculture is carried out for multiple times to obtain a denitrification and phosphorus accumulation microbial suspension; and the obtained denitrification and phosphorus accumulation microbial suspension is implanted into uranium-containing wastewater, and reaction is carried out at normal temperature under a sealed condition to remove fixed uranium. The denitrification and phosphorus accumulation microorganism decomposes organic phosphorus such as phytic acid into phosphate, andthen induces U-P to form scaly crystalline precipitate, so that uranium is fixed.

Description

technical field [0001] The invention relates to a method for removing fixed uranium by utilizing the denitrification and phosphorus accumulation of microorganisms, and belongs to the technical field of restoration of polluted water bodies in uranium mining areas. Background technique [0002] Various links such as uranium mining and smelting, processing and utilization, and waste treatment and disposal have caused varying degrees of impact on the ecological environment. The "in situ immobilization" of dissolved uranium and other elements into the mineralized lattice through the mediation of microorganisms is considered as a potential method to prevent the further migration and diffusion of uranium and other elements because of its safety and environmental protection advantages. At present, in the comprehensive promotion of ecological civilization construction and environmental protection, the restoration and management of the ecological environment in mining areas has become...

Claims

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Application Information

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IPC IPC(8): C02F3/34G21F9/18C02F101/16
CPCC02F3/34G21F9/18C02F2101/006C02F2101/163
Inventor 谭文发唐东山王亚超吕俊文王红强谢超黎媛彭雷寸金枝
Owner NANHUA UNIV
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