Reduced strain Ylb10 and application thereof in reducing Cr (VI)

A strain, typical technology, applied in bacteria, microorganism-based methods, restoration of polluted soil, etc., can solve problems such as microbial poisoning, inability to effectively reduce chromium, etc.

Active Publication Date: 2020-11-27
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Cr(VI) is toxic to microorganisms, and many microorganisms cannot effectively reduce chromium or grow in a high-concentration Cr(VI) environment. Therefore, high-performance Cr(VI) is isolated from the environment or obtained through genetic engineering. Reducing bacterial strains is still an important topic in the field of heavy metal bioremediation

Method used

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  • Reduced strain Ylb10 and application thereof in reducing Cr (VI)
  • Reduced strain Ylb10 and application thereof in reducing Cr (VI)
  • Reduced strain Ylb10 and application thereof in reducing Cr (VI)

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation and purification of Ylb10

[0023] YEM001 is a group of bacteria that can effectively reduce Cr(VI). It was obtained from the bottom mud of the Qiusuo River in China Three Gorges University and PCS culture solution (containing Cr(VI)) through 5 generations of enrichment culture.

[0024] Using the YEM001 bacterial population as the source of the isolated bacteria, the Cr(VI)-reducing strain was isolated and purified by the gradient dilution coating plate method, and finally the pure culture strain Ylb10 with Cr(VI) ability was obtained. The medium used for isolation and cultivation is PCS-Cr medium, the main components are: peptone 5g / L, yeast extract powder 1g / L, sodium chloride 5g / L, pH=8.5-9.0, K 2 Cr 2 o 7 100mg / L. The strains were cultured at 28°C for biochemical culture. For solid medium, add 1.5% agar powder (Suolaibao, Beijing).

[0025] The strain morphology and Gram staining results of the obtained strain Ylb10 are shown in figure 1 .The result...

Embodiment 2

[0027] Phylogenetic analysis of Ylb10 strain

[0028] The Ylb10 bacteria were cultured for about 48 hours, and the 3OD bacterial volume was collected, and the total DNA of the bacteria was extracted, and the obtained DNA was analyzed by 16S rDNA sequencing. The sequencing results were compared with the existing 16S rDNA sequences in the NCBI database, and the phylogenetic tree was analyzed using MEGA X. The phylogenetic tree constructed is shown in figure 2 . The results showed that the 16S rDNA sequence of the strain was 98.07% similar to the 16S rDNA sequence of Alicyclophilus denitrificans in the database. The strain was identified as Alicycliphilus denitrificans.

Embodiment 3

[0030] Growth Characteristics of Ylb10 Bacteria

[0031] The growth trend of Ylb10 bacteria in the PCS medium without Cr(VI) is shown in image 3 . Inoculate Ylb10 in fresh PCS culture medium, the initial OD is 0.15, the strain starts to recover and grow within a short time, and ends the exponential growth phase within 40 hours, and enters the stable growth phase. 87h into the decline period. It shows that the bacterium has relatively rapid growth ability, which is beneficial to the treatment of environmental pollutants.

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Abstract

The invention provides a strain Alicycliphilus sp. Ylb10 which is preserved in China Center for Type Culture Collection on August 7th, 2020, the preservation number is CCTCC M 2020415, and the preservation address is Wuhan University, Wuhan, China. The strain Ylb10 is applied to restoration of Cr (VI) heavy metal pollution and reduction of Cr (VI) into Cr (III). The strain has good Cr (VI) reducing capacity, and when the concentration of environmental Cr (VI) is 50 mg/L, 96.45% of Cr (VI) is reduced within 18 hours. When the concentration of Cr (VI) in the environment is 200 mg/L, the reduction rate of Cr (VI) in 60 hours reaches 99.06%. The pH value of the optimal reduction Cr (VI) of the strain is 8-9, and the culture condition is static culture. The strain can utilize L-alanine, L-aspartic acid, L-glutamic acid, L-serine and other amino acids, and alpha-hydroxy-butyric acid, L-lactic acid, propionic acid, acetic acid and other organic acids. The Ylb10 strain is an effective hexavalent chromium reducing strain and can be applied to the field of bioremediation of chromium-polluted water samples and soil.

Description

technical field [0001] The invention relates to a bacterial strain Alicycliphilus sp. Ylb10 and its application in reducing Cr(VI), belonging to the field of biotechnology. Background technique [0002] In recent years, with the development of chromium industries such as chromium salts, leather, printing and dyeing, and electroplating, the incidents of soil chromium (Cr) pollution have gradually increased, and the environmental pollution caused by chromium has become more and more serious. threaten human health. Cr(VI) is easily soluble in water and has strong oxidizing properties. Long-term exposure of the human body to heavy metal chromium pollution can cause damage to the nervous system, liver, and kidneys. It is listed as a carcinogen, and skin contact may cause allergies or genetic defects. (VI) It is also a persistent hazard to the environment. Cr(III) has low solubility and weak mobility. It is an essential trace element for human and animal bodies, and is mainly in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/22
CPCC02F3/34B09C1/10C02F2101/22C12R2001/01C12N1/205
Inventor 吕育财左群郭金玲任立伟龚大春
Owner CHINA THREE GORGES UNIV
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