A kind of extraction method of collagen in bovine bone

Abstract

The invention relates to the technical field of bovine bone extraction, in particular to a method for extracting collagen from bovine bones. The method comprises the following steps: S1, crushing; S2,quick freezing; S3, unfreezing; S4, degreasing and decalcifying; and S5, frequency-conversion electrolysis: putting bovine bone powder into an amino acid solution, carrying out frequency-conversion electrolysis for 20-25 minutes under a current density of 15-25 A / cm<2>, performing standing, adding modified chitosan, performing stirring for 10-20 minutes at 30-40 DEG C, then performing centrifuging for 8-15 minutes in a centrifugal machine with a rotating speed of 4000-6000 r / min, taking supernate, and concentrating the supernate by 3-5 times, performing freeze drying to obtain the collagen. The method for extracting collagen from bovine bones has the advantages of high extraction rate, excellent collagen thermal stability and high transparency, and can prevent amino acids, polysaccharidesand other components in bovine bones from being destroyed.

Description

technical field [0001] The invention relates to the technical field of bovine bone extraction, in particular to a method for extracting collagen in bovine bone. Background technique [0002] Bovine bone is a by-product of cattle, accounting for 20% to 30% of the total animal weight, mainly composed of bone, periosteum and bone marrow, which is rich in protein, fat, chondroitin, calcium, iron, phosphorus, Zinc and other minerals and vitamins. [0003] Collagen is a fibrous protein polymerized with a triple helix structure. It is an important structural protein in organisms. It can support organs and protect the body. It is widely used in health care products, biological drugs, cosmetics and food industries. Therefore, the extraction and deep processing of bovine collagen has become a research hotspot in recent years. Patent No. CN201310406154.6 discloses a processing technology for extracting composite collagen from yak bones. This patent effectively extracts collagen from ...

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Problems solved by technology

Patent No. CN201310406154.6 discloses a processing technology for extracting composite collagen from yak bones. This patent effectively extracts collagen from yak bones, but acid leaching and alkali neutralization easily lead to irreversible denaturation of proteins

[0004] At present, the commonly used collagen extraction methods at home and abroad mainly include hot water extraction, acid extraction, alkali extraction and enzyme extraction, but the yield of hot water extraction is low; the acid method is completely hydrolyzed, and tryptophan is completely destroyed. Serine and tyrosine will also be partially destroyed; extracting collagen under alkaline conditions can easily cause protein denaturation, such as collagen peptide bond hydrolysis, all amino acids containing hydroxyl and sulfhydryl groups will be destroyed, and the resulting product has a low isoelectric point pH value. Asparagine and glutamine are converted into aspartic acid and glutamic acid respectively. If the hydrolysis is severe, a racemic mixture of D and L amino acids will be produced. If the content of D amino acids is higher than that of L amino acids, it will Inhibit the absorption of L-type amino acids, some D-type amino acids are toxic, and some even have carcinogenic, teratogenic and mutagenic effects; enzymatic hydrolysis can not only hydrolyze the terminal peptide of collagen fibrils, improve the yield of collagen, but also It will not destroy the triple helical structure of collagen, and can maintain its excellent physical and biochemical properties, so enzymatic hydrolysis is an ideal method at present, but because the calcium in the bone structure of animals mainly forms bone salt deposits with hydroxyapatite and collagen On the surface of collagen, calcium will reduce the activity of pepsin and affect the extraction rate of collagen protein. At the same time, the fat content in bovine bone is high. During the enzymatic hydrolysis process, the deep insoluble calcium hygronate will be deposited on the bone surface, affecting the enzymatic hydrolysis reaction. and affect the color and clarity of collagen

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