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A kind of bionic double-layer dressing and preparation method thereof

A double-layer, small intestinal submucosa technology, which is applied in the field of human skin tissue engineering materials, can solve the problems of long culture time and complicated preparation process, and achieve the effects of short time consumption, accelerated healing rate, and improved healing speed

Active Publication Date: 2021-07-06
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation process is complicated and the culture time is long, at least the artificial skin needs to be cultured in the culture solution for 9 days

Method used

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  • A kind of bionic double-layer dressing and preparation method thereof
  • A kind of bionic double-layer dressing and preparation method thereof
  • A kind of bionic double-layer dressing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A preparation method of a bionic double-layer dressing is prepared according to the following steps:

[0056] Step 1, SIS membrane preparation: take fresh pig small intestine, first remove the serosal layer and muscular layer by mechanical means; the specific method in this example is: first extrude the contents of fresh pig small intestine, cut into small pieces about 15 cm long, The mesentery attached to the surface was torn off, placed in a stainless steel container, and rinsed several times with tap water; the pig small intestine after cleaning was spread on a board, and the mucosal layer, serosa layer and muscle layer. Until the translucent submucosa of the small intestine is exposed; after repeated washing with tap water several times, the small intestine is penetrated and turned over with chopsticks, and the residual tissue is further scraped; finally, the small intestine is cut into small pieces of about 10cm×15cm along the longitudinal axis with scissors, and t...

Embodiment 2

[0062] A preparation method of a bionic double-layer dressing is prepared according to the following steps:

[0063] Step 1, SIS membrane preparation: Take fresh sheep small intestine, remove the serosal layer and muscular layer by mechanical means; then soak it in a mixture of methanol and chloroform with a volume ratio of 1.5:1 for 15 hours, and degrease it with deionized Rinse with water for 3 times; then place the sheep small intestine after rinsing in a mixed system of 0.08% trypsin and 0.1% ethylenediaminetetraacetic acid to digest at room temperature for 10 hours; wash with purified water for 3 times; then wash with the aforementioned water After the sheep small intestine was shaken in physiological saline containing 0.5% sodium lauryl sulfate for 8 hours, the SIS membrane was obtained; the obtained SIS membrane was washed with physiological saline for 3 times, and stored in an environment at 4°C for later use;

[0064] Step 2, SIS powder preparation: Soak the SIS film ...

Embodiment 3-8

[0069] With reference to Example 1 or Example 2, according to the process parameters in Table 1, a bionic double-layer dressing was prepared;

[0070] Process parameter in table 1 embodiment 3-8

[0071]

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Abstract

The invention provides a bionic double-layer dressing, which comprises a decellularized animal small intestinal submucosa and a gel layer cross-linked on the film layer, and the film layer and the gel layer together form a bionic double-layer dressing with a double-layer structure; The preparation steps include preparation of decellularized small intestinal submucosa, preparation of decellularized small intestinal submucosa powder, preparation of decellularized small intestinal submucosa solution and preparation of bionic double-layer dressing. To the surface of the submucosa of the decellularized small intestine, the bionic double-layer dressing was obtained after freezing and cross-linking. The bionic double-layer dressing prepared by the invention not only has excellent tensile properties, but also has a semi-permeable barrier, which can prevent water evaporation and bacterial penetration. At the same time, the bionic double-layer dressing prepared by the invention is beneficial to the healing of the wound, can significantly increase the healing speed of the wound, and the wound healing rate is obviously accelerated on the 3rd day and the 7th day after the operation.

Description

technical field [0001] The invention belongs to the field of human skin tissue engineering materials, in particular to a bionic double-layer dressing and a preparation method thereof. Background technique [0002] Human skin consists of three layers: epidermis, dermis, and subcutaneous tissue. The epidermis is used to prevent the extravasation of water and electrolytes, the intrusion of pathogenic microorganisms and chemical substances, and at the same time breathe and perspire through the skin appendages; the dermis is composed of fibers, matrix and cells, and the matrix mainly provides physical support for cells and a place for material metabolism; subcutaneous The tissue is located in the lower part of the dermis and is composed of loose connective tissue and fat lobules, which are used to prevent heat dissipation, store energy and resist external mechanical shocks. [0003] Bionic double-layer dressing, which can be called artificial skin or bionic skin, mainly uses tis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/60A61L27/40A61L27/36A61L27/56A61L27/50
CPCA61L27/3629A61L27/50A61L27/56A61L27/60A61L2400/16A61L2430/40
Inventor 王量廖建贵王文平曹川李世荣
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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