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Culture medium for enriching lactobacilli and preparation method of culture medium

A lactobacillus and lactic acid enrichment technology, applied in the field of microbial culture, can solve problems such as inability to achieve rapid detection, and achieve the effects of enrichment, increased yield, and increased growth vigor

Inactive Publication Date: 2017-11-10
QINGDAO AMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing enrichment medium can only enrich 1 or 2 kinds of pathogenic bacteria in a single enrichment, and cannot achieve the purpose of rapid detection. Therefore, in order to realize the rapid enrichment detection of pathogenic bacteria, it is necessary method to improve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A culture medium for enriching Lactobacillus, comprising basal culture medium and additives, in parts by mass, said additives comprising the following substances:

[0026] 8 parts of soybean peptone, 6 parts of sodium chloride, 35 parts of mutton soup, 48 parts of rice fermentation liquid, 8 parts of milk powder, 3 parts of glucose, 6 parts of lactose, 3 parts of phosphorus source, 0.08 parts of pig bile salt, 1.8 parts of sodium pyruvate , 2.6 parts of glycine, 1.6 parts of β-lactam antibiotics, 0.3 parts of iron salt, 0.02 parts of surfactant, 500 parts of distilled water;

[0027] The present embodiment also provides a method for preparing the medium for enriching Lactobacillus, comprising the following steps:

[0028] Preparation of mutton soup: Put the mutton into 3 times the water, then raise the temperature to 100°C at a rate of 4°C / min, then keep it warm for 60 minutes, and cool to room temperature to obtain mutton soup;

[0029] Preparation of rice fermentatio...

Embodiment 2

[0032] A culture medium for enriching Lactobacillus, comprising basal culture medium and additives, in parts by mass, said additives comprising the following substances:

[0033] 5 parts of blood peptone, 5 parts of sodium chloride, 25 parts of mutton soup, 36 parts of corn fermentation liquid, 8 parts of milk powder, 3 parts of glucose, 2 parts of lactose, 2.4 parts of phosphorus source, 0.05 parts of pig bile salt, 1.2 parts of sodium pyruvate , 0.5 parts of L-alanine, 1.2 parts of aminoglycoside antibiotics, 0.1 part of chromium salt, 0.01 part of surfactant, 500 parts of distilled water;

[0034]The present embodiment also provides a method for preparing the medium for enriching Lactobacillus, comprising the following steps:

[0035] Preparation of mutton soup: Put the mutton into twice the amount of water, then raise the temperature to 85°C at a rate of 3°C / min, then keep it warm for 30 minutes, and cool to room temperature to obtain mutton soup;

[0036] Preparation of ...

Embodiment 3

[0039] A culture medium for enriching Lactobacillus, comprising basal culture medium and additives, in parts by mass, said additives comprising the following substances:

[0040] 10 parts of tryptone, 10 parts of sodium chloride, 42 parts of chicken broth, 64 parts of sorghum fermentation liquid, 10 parts of milk powder, 5 parts of glucose, 8 parts of lactose, 3.8 parts of phosphorus source, 0.12 parts of pig bile salt, 2.4 parts of sodium pyruvate , 0.5-3.6 parts of L-glutamine, 1.8 parts of polypeptide antibiotics, 0.4 parts of cobalt salt, 0.03 parts of surfactant, 500 parts of distilled water;

[0041] The present embodiment also provides a method for preparing the medium for enriching Lactobacillus, comprising the following steps:

[0042] Preparation of chicken broth: Put the chicken into 4 times the water, then raise the temperature to 85°C at a rate of 3°C / min, then keep it warm for 30min, and cool down to room temperature to obtain chicken broth;

[0043] Preparation...

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Abstract

The invention relates to the technical field of microbial culture and particularly relates to a culture medium for enriching lactobacilli and a preparation method of the culture medium. The culture medium for enriching lactobacilli comprises a basal culture medium and an additive; and the additive comprises the following substances in parts by mass: 2-12 parts of peptone, 3-12 parts of sodium chloride, 20-50 parts of broth, 20-80 parts of cereal fermentation broth, 5-12 parts of milk powder, 2-8 parts of glucose, 1-10 parts of lactose, 1.2-5.6 parts of phosphorus sources, 0.02-0.15 part of pig bile salt, 0.6-2.9 parts of sodium pyruvate, 0.1-3.8 parts of amino acid, 0.1-2 parts of antibiotics, 0.05-0.5 part of metal salt, 0.01-0.05 part of a surfactant and 500 parts of distilled water. According to the culture medium, the selective growth of lactobacilli is realized by firstly inhibiting the growth of other bacteria by the cereal fermentation broth; abundant nutrient substances are added into the culture medium to enhance the growth vigor of the lactobacilli and promote the growth of the lactobacilli, so that the yield of the lactobacilli is increased, and the enrichment of the lactobacilli is realized; and the culture medium has a wide application prospect in industrial production and detection.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a culture medium for enriching lactobacilli and a preparation method thereof. Background technique [0002] Lactic acid bacteria refers to the general term for a class of non-spore-forming, Gram-positive bacteria whose main product is lactic acid. All bacteria that can produce lactic acid from the fermentation of glucose or lactose are collectively called lactic acid bacteria. This is a rather complex group of bacteria, which can be divided into at least 18 genera and more than 200 species. Except for a few, most of them are essential flora in the human body and have important physiological functions, which widely exist in the intestinal tract of the human body. At present, it has been confirmed by domestic and foreign biologists that intestinal lactic acid bacteria have a very close and direct relationship with health and longevity. At present, the detection of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04C12R1/225
CPCC12N1/20C12Q1/045
Inventor 翟岱青
Owner QINGDAO AMA CO LTD
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